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The effects of three total mixed rations with different concentrate to maize silage ratios and different levels of microalgae Chlorella vulgaris on in vitro total gas, methane and carbon dioxide production

Published online by Cambridge University Press:  02 November 2016

A. E. KHOLIF ,
M. M. Y. ELGHANDOUR ,
A. Z. M. SALEM ,
A. BARBABOSA ,
O. MÁRQUEZ  and
N. E. ODONGO
A. E. KHOLIF
Affiliation:
Dairy Science Department, National Research Centre, 33 Bohouth St. Dokki, Giza, Egypt
M. M. Y. ELGHANDOUR
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, México
A. Z. M. SALEM*
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, México
A. BARBABOSA
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, México
O. MÁRQUEZ
Affiliation:
Centro Universitario Amecameca, Universidad Autónoma del Estado de México, Amecameca, México
N. E. ODONGO
Affiliation:
Department of Animal Sciences, School of Agriculture, Pwani University, P.O. Box 195-80108, Kilifi, Kenya
*
*To whom all correspondence should be addressed. Email: asalem70@yahoo.com
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Summary

The aim of the current study was to assess the effects of adding Chlorella vulgaris algae at different levels on in vitro gas production (GP) of three total mixed rations (TMR) with different concentrate (C): maize silage (S) ratios (25C : 75S, 50C : 50S, 75C : 25S). Chlorella vulgaris was added at 0, 20, 40 and 80 mg/g dry matter (DM) of the TMR and total gas, methane (CH4) and carbon dioxide (CO2) production were recorded after 2, 4, 6, 8, 10, 12, 24 and 48 h of incubation in three runs. Increasing concentrate portion in the TMR linearly increased the asymptotic GP and decreased the rate of GP without affecting the lag time. Addition of C. vulgaris at 20 mg/g DM to the 25C : 75S TMR increased the asymptotic GP, CH4, CO2 and GP at 48 h. Addition of C. vulgaris to the 50C : 50S TMR decreased the asymptotic GP and GP at 48 h. Higher CH4 production was observed at 48 h of incubation when C. vulgaris was included at (per g DM): 20 mg for the 25C : 75S ration, 40 mg for the 50C : 50S ration and 80 mg for the 75C : 25S ration. Inclusion of C. vulgaris linearly increased CH4 production for the 50C : 50S ration and increased CO2 production at 10 and 12 h of incubation for the 50C : 50S ration, whereas 20 and 40 mg C. vulgaris/g DM of the 75C : 25S TMR decreased CO2 production. The 25C : 75S TMR had the highest in vitro DM disappearance with C. vulgaris addition. Chlorella vulgaris addition was more effective with rations high in fibre content than those high in concentrates. It can be concluded that the optimal level of C. vulgaris addition was 20 mg/g DM for improved ruminal fermentation of the 25C : 75S TMR.

Type
Animal Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 3 , April 2017 , pp. 494 - 507
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Copyright
Copyright © Cambridge University Press 2016 

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