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Effects of maternal nutrition and plasma progesterone concentrations on survival and growth of the sheep embryo in early gestation

Published online by Cambridge University Press:  27 March 2009

R. A. Parr ,
I. A. Cumming  and
I. J. Clarke
R. A. Parr
Affiliation:
Victorian Department of Agriculture, Animal Research Institute, Werribee, Victoria, Australia 3030
I. A. Cumming
Affiliation:
Victorian Department of Agriculture, Animal Research Institute, Werribee, Victoria, Australia 3030
I. J. Clarke
Affiliation:
Medical Research Centre, Prince Henry's Hospital, Melbourne, Victoria, Australia 3000
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Summary

Mature Merino ewes (n = 320) were fed either maintenance (100% M) or 25% maintenance (25% M) rations from the day after mating until embryos were collected at either day 11 or 21 after mating. On the 4th day after mating 223 ewes were ovariectomized and given 5, 10, 15, 20 or 25 mg progesterone/day (intramuscular) until embryo collection. A further group of sham operated ewes (n = 54) acted as controls. Despite live-weight losses of 10·7 and 15·8% from mating to days 11 and 21 after mating respectively, ewes on 25% M rations showed no evidence that embryonic survival was influenced by undernutrition. The embryos from ewes fed 25 % M rations were significantly (P < 0·05) smaller than those fed 100% M (crown-rump measurements were 5·93 ± 0·139 mm and 6·39 ± 0·116 mm at day 21 after mating in the 25 % M and 100% M groups respectively). Development, assessed by formation of the first pharangeal lobe and the fore- and hind-limb buds, was significantly retarded (P < 0·05) in the 21-day-old embryos from the undernourished ewes. Embryo survival in ovariectomized ewes receiving 5 or 10 mg progesterone/day was significantly (P < 0·05) lower than in ovariectomized ewes receiving 20 and 25 mg progesterone/ day at day 11 after mating. Embryo survival at both days 11 and 21 after mating increased with increasing doses of progesterone. Ewes fed 25% M rations had elevated (P< 0·001) concentrations of peripheral progesterone compared with those fed 100% M. This occurred when the progesterone was derived from either exogenous or endogenous (ovarian) sources, dismissing the possibility that changes in secretion rates could account for these observed differences. Differences in embryo growth and development were not related to these variations in the plasma progesterone concentration. Undernutrition restricted both growth and development to 21 days of pregnancy.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 98 , Issue 1 , February 1982 , pp. 39 - 46
Copyright
Copyright © Cambridge University Press 1982

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References

REFERENCES

Bennett, D., Axelsen, A. & Chapman, H. W. (1964). The effect of nutritional restriction during early pregnancy on numbers of lambs born. Proceedings of the Australian Society of Animal Production 5, 70–71.Google Scholar
Bennett, D., Nadin, J. B. & Axelsen, A. (1970). The effect of undernutrition during early pregnancy in Merino ewes. Proceedings of the Australian Society of Animal Production 8, 362–365.Google Scholar
Bensadoun, A. & Reid, J. T. (1962). Estimation of rate of portal blood flow in ruminants; effect of feeding, fasting and anaesthesia. Journal of Dairy Science 45, 540–543.CrossRefGoogle Scholar
Blockey, M. A. de B., Cumming, I. A. & Baxter, R. W. (1974). The effect of short-term fasting in ewes on early embryonic survival. Proceedings of the Australian Society of Animal Production 10, 265–269.Google Scholar
Burger, H. G., Lee, V. W. K. & Rennie, G. D. (1972). A generalized computer program for the treatment of data from competitive protein binding assays including radioimmunoassay. Journal of Laboratory and Clinical Medicine 80, 302–312.Google Scholar
Cumming, I. A., Blockey, M. A. de B., Winfield, C. G., Parr, R. A. & Williams, A. H. (1975). A study of relationships of breed, time of mating, level of nutrition, live weight, body condition, and face cover to embryo survival in ewes. Journal of Agricultural Science, Cambridge 84, 559–565.Google Scholar
Cumming, I. A., Mole, B. J., Obst, J., Blockey, M. A. de B., Winpield, C. G., & Goding, J. R. (1971). Increase in plasma progesterone caused by undernutrition during early pregnancy in the ewe. Journal of Reproduction and Fertility 24, 146–147.CrossRefGoogle ScholarPubMed
Draper, N. R. & Smith, H. (1966). Applied Regression Analysis. New York: Wiley.Google Scholar
Edey, T. N. (1966). Nutritional stress and preimplantation embryonic mortality in Merino sheep. Journal of Agricultural Science, Cambridge 67, 287–293.CrossRefGoogle Scholar
Edey, T. N. (1970 a). Nutritional stress and preimplantation embryonic mortality in Merino sheep, 1965. Journal of Agricultural Science, Cambridge 74, 181–186.CrossRefGoogle Scholar
Edey, T. N. (1970 b). Nutritional stress and preimplantation embryonic mortality in Merino sheep,1966. Journal of Agricultural Science, Cambridge 74, 187–192.Google Scholar
Edey, T. N. (1970 C). Nutritional stress and preimplantation embryonic mortality in Merino sheep,1967. Journal of Agricultural Science, Cambridge 74, 193–198.Google Scholar
Edey, T. N. (1976 a). Nutrition and embryo survival in the ewe. Proceedings of the New Zealand Society of Animal Production 36, 231–239.Google Scholar
Edey, T. N. (1976 b). Embryo mortality. In ‘Sheep Breeding’. Proceedings of the 1976 International Congress, Muresk (ed. Tomes, G. J., Robertson, D. E. and Lightfoot, R. J.), pp. 400–410. Western Australian Institute of Technology.Google Scholar
Eveeitt, G. C. (1967). Residual effects of prenatal nutrition on the postnatal performance of Merino sheep. Proceedings of the New Zealand Society of Animal Production 27, 52–68.Google Scholar
Goding, J. R., Baird, D. T., Cumming, I. A. & Mccracken, J. A. (1971). Functional assessment of autotransplanted endocrine organs. Karolinska Symposia on Research Methods in Reproductive Endocrinology. 4th Symposium on Perfusion Techniques (ed. Dicsfalusy, E.), pp. 169–191.Google Scholar
Green, W. W. & Winters, L. M. (1945). Prenatal development of the shoop. Technical Bulletin 169. University of Minnesota, Agricultural Experiment Station.Google Scholar
Gunn, R. G. & Doney, J. M. (1973). The effects of nutrition and rainfall at the time of mating on the reproductive performance of ewes. Journal of Reproduction and Fertility. Supplement 19, 253–258.Google ScholarPubMed
Harvey, W. R. (1960). Least Squares Analysis of Data with Unequal Subclass Numbers, pp. 20–28. Agricultural Research Service. USDA.Google Scholar
Hossain, M. I., Lee, C. S., Clarke, I. J. & O'Shea, J. D. (1979). Ovarian and luteal blood flow and peripheral plasma progesterone lovels in cycling guinea pigs. Journal of Reproduction and Fertility 57’ 167–174.Google Scholar
Jefferies, B. C. (1961). Body condition scoring and its use in management. Tasmanian Journal of Agriculture 32, 19–21.Google Scholar
Killeen, I. D. (1974). Effects of liveweight of Border Leicester x Merino ewes and breed of ram on fertilization in flock-mated sheep. Journal of Reproduction and Fertility 36, 464–465.CrossRefGoogle Scholar
Moore, R. M. & Rowson, L. E. A. (1966). The corpus luteum of the sheep: functional relationship between the embryos and corpus luteum. Journal of Endocrinology 34, 233–239.CrossRefGoogle Scholar
Moule, G. R. (1954). Observations on mortality amongst lambs in Queensland. Australian Veterinary Journal 30, 153–171.Google Scholar
Radford, H. M., Watson, R. H. & Wood, G. F. (1960). A crayon and associated harness for detection of mating under field conditions. Australian Veterinary Journal 36, 57–66.CrossRefGoogle Scholar
Schinkel, P. G. & Short, B. V. (1961). The influence of nutritional lovol during pro-natal life on adult fleece and body characters. Australian Journal of Agricultural Research 12, 176–202.CrossRefGoogle Scholar
SOkal, R. R. & Rohlf, F. J. (1969). Biometry. San Francisco: W. H. Freeman and CoGoogle Scholar
Van Niekerk, C. H., Belonje, P. C. & Hunter, G. L. (1968). Early embryonic mortality and resorption in Merino ewes due to malnutrition. Proceedings of the 6th International Congress of Animal Reproduction and Artificial Insemination, Paris 1, 455–458.Google Scholar
Wilson, J. B. (1976). Teddyboar Statistical Programme. Technical Report, edition 2.Dunedin, N.Z.: Otago University.Google Scholar