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Effects of egg-yolk-citrate and egg-yolk-glycine dilutors on the morphology of bovine spermatozoa

Published online by Cambridge University Press:  27 March 2009

D. P. Mukherjee  and
H. M. Dott
D. P. Mukherjee
Affiliation:
A.R.C. Unit of Reproductive Physiology and Biochemistry, Animal Research Station, Huntingdon Road, Cambridge
H. M. Dott
Affiliation:
A.R.C. Unit of Reproductive Physiology and Biochemistry, Animal Research Station, Huntingdon Road, Cambridge
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Extract

Twenty semen samples were collected by means of the artificial vagina from three Friesian and two Dexter bulls. In each sample, the head length and head breadth of spermatozoa which did not take up the nigrosin-eosin stain, and their percentage before and after 72 hr. of preservation in egg-yolk-citrate and egg-yolk-glycine, were studied. The following results were obtained from the study:

1. No significant variation was found in the two measurements of sperm head before and after preservation in E.Y.C.

2. Both the head length and head breadth of spermatozoa preserved in E.Y.G. were significantly less than those before and after preservation in E.Y.C..

3. The change in size of the spermatozoa head in E.Y.G.. dilutor may be due to an alteration in the acrosome.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 55 , Issue 2 , October 1960 , pp. 225 - 228
Copyright
Copyright © Cambridge University Press 1960

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References

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