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Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects

  • G. C. Waghorn (a1), I. D. Shelton (a1), W. C. McNabb (a2) and S. N. McCutcheon (a2)


Fourteen young wether sheep were fed freshly cut Lotus pedunculatus as a sole diet to examine the effects of condensed tannins (CT; 55 g/kg lotus DM) on nitrogenous aspects of digestion. The experiment was carried out indoors at Palmerston North, New Zealand over 32 days with one group of sheep receiving an intraruminal infusion of polyethylene glycol (PEG; 100 g/day) to preferentially bind CT (PEG group) so that the lotus was essentially ‘CT-free'. The other sheep, not given PEG, were termed the ‘Tannin’ group.

The principal effects of CT were to increase the flow of feed nitrogen (N) to the abomasum despite a 12% reduction in DM intake of the Tannin sheep. Rumen microbial N turnover rate was slower in Tannin animals than in those receiving PEG (1·86 v. 2·63/day) but microbial N flux to the abomasum was similar in both treatments. The proportion of N intake disappearing from the rumen was lower in Tannin (0·13) than in PEG sheep (0·26) and the N digestibility was 0·67 and 0·81 for the respective treatments (P < 0·001).

The beneficial effects of CT in reducing rumen degradation of feed protein were negated in part by a reduction in fractional absorption of amino acids (AA) from the small intestine. Fractional absorption of essential AA was 0·66 in Tannin and 0·79 in PEG sheep; values for non-essential AA were 0'59 in Tannin and 0·73 in PEG groups. Amino acid concentrations in blood were similar for both groups, but Tannin sheep had lower plasma urea concentrations, a more rapid plasma urea turnover rate and a higher irreversible loss than those receiving PEG. Growth hormone concentrations in plasma were similar for both treatments.



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Barry, T. N. & Duncan, S. J. (1984). The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. 1. Voluntary intake. British Journal of Nutrition 51, 485491.
Barry, T. N. & Forss, D. A. (1983). The condensed tannins content of vegetative Lotus pedunculatus, its regulation by fertiliser application, and effect upon protein solubility. Journal of the Science of Food and Agriculture 34, 10471056.
Barry, T. N. & Manley, T. R. (1984). The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. 2. Quantitative digestion of carbohydrates and proteins. British Journal of Nutrition 51, 493504.
Blytt, H. J., Guscar, T. K. & Butler, L. G. (1988). Antinutritional effects and ecological significance of dietary condensed tannins may not be due to binding and inhibiting digestive enzymes. Journal of Chemical Ecology 14, 14551465.
Cocimano, M. R. & Leng, R. A. (1967). Metabolism of urea in sheep. British Journal of Nutrition 21, 353371.
Cotta, M. A. & Hespell, R. B. (1986). Proteolytic activity of the ruminal bacterium Butyrivibrio fibrisolvens. Applied and Environmental Microbiology 52, 5158.
Egan, A. R. & Ulyatt, M. J. (1980). Quantitative digestion of fresh herbage by sheep. VI. Utilization of nitrogen in five herbages. Journal of Agricultural Science, Cambridge 94, 4756.
Egan, A. R., Boda, K. & Varady, J. (1986). Regulation of nitrogen metabolism and recycling. In Control of Digestion and Metabolism in Ruminants (Eds Milligan, L. P., Grovum, W. L. & Dobson, A.), pp. 386402. Englewood Cliffs: Prentice-Hall.
Flux, D. S., MacKenzie, D. D. S. & Wilson, G. F. (1984). Plasma metabolite and hormone concentrations in Friesian cows of differing genetic merit measured at two feeding levels. Animal Production 38, 377384.
Horigome, T., Kumar, R. & Okamoto, K. (1988). Effects of condensed tannins prepared from leaves of fodder plants on digestive enzymes in vitro and in the intestine of rats. British Journal of Nutrition 60, 275285.
Jones, W. T. & Mangan, J. L. (1977). Complexes of the condensed tannins of sainfoin (Onobrychis viciifolia Scop.) with Fraction 1 leaf protein and with submaxillary mucoprotein, and their reversal by polyethylene glycol and pH. Journal of the Science of Food and Agriculture 28, 126136.
Kumar, R. & Vaithiyanathan, S. (1990). Occurrence, nutritional significance and effect on animal productivity of tannins in tree leaves. Animal Feed Science and Technology 30, 2138.
Mangan, J. L. (1988). Nutritional effects of tannins in animal feeds. Nutrition Research Reviews 1, 209231.
Marsh, W. H., Fingerhut, B. & Miller, H. (1965). Automated and manual direct methods for the determination of blood urea. Clinical Chemistry 11, 624627.
McNabb, W. C., Waghorn, G. C., Barry, T. N. & Shelton, I. D. (1993). The effect of condensed tannins in Lotus pedunculatus upon the digestion and metabolism of methionine, cystine and inorganic sulphur in sheep. British Journal of Nutrition 70, 647661.
McWilliam, J. R. (1973). Plant factors, environment and bloat. In Bloat (Eds Leng, R. A. & McWilliam, J. R.), pp. 3338. Armidale: University of New England Publishing Unit.
Niezen, J. H., Waghorn, T. S., Waghorn, G. C. & Charleston, W. A. G. (1993). Internal parasites and lamb production – a role for plants containing condensed tannins? Proceedings of the New Zealand Society of Animal Production 53, 235238.
Nolan, J. V. (1975). Quantitative models of nitrogen metabolism in sheep. In Digestion and Metabolism in the Ruminant (Eds McDonald, I. W. & Warner, A. C. I.), pp. 416471. Armidale: University of New England Publishing Unit.
Nolan, J. V. & Leng, R. A. (1972). Dynamic aspects of ammonia and urea metabolism in sheep. British Journal of Nutrition 27, 177194.
Ørskov, E. R. (Ed.) (1982). Protein Nutrition in Ruminants. London: Academic Press.
Pritchard, D. A., Stocks, D. C., O'sullivan, B. M., Martin, P. R., Hurwood, I. S. & O'Rourke, P. K. (1988). The effect of polyethylene glycol (PEG) on wool growth and liveweight of sheep consuming a mulga (Acacia aneura) diet. Proceedings of the Australian Society of Animal Production 17, 290293.
Purchas, R. W. & Keogh, R. G. (1984). Fatness of lambs grazed on ‘Grasslands Maku’ lotus and ‘Grasslands Huia’ white clover. Proceedings of the New Zealand Society of Animal Production 44, 219221.
Reid, C. S. W., Ulyatt, M. J. & Wilson, J. M. (1974). Plant tannins, bloat and nutritive value. Proceedings of the New Zealand Society of Animal Production 34, 8293.
Reis, P. J. (1979). Effects of amino acids on the growth and properties of wool. In Physiological and Environmental Limitations to Wool Growth (Eds Black, J. L. & Reis, P. J.), pp. 223242. Armidale: University of New England Publishing Unit.
Sarkar, S. K., Howarth, R. E. & Goplen, B. P. (1976). Condensed tannins in herbaceous legumes. Crop Science 16, 543546.
Satter, L. D. & Slyter, L. L. (1974). Effect of ammonia concentration on rumen microbial protein production in vitro. British Journal of Nutrition 32, 199208.
Shipley, R. A. & Clark, R. E. (Eds) (1972). Tracer Methods for in vivo Kinetics: Theory and Applications. New York: Academic Press.
Tagari, H. & Bergman, E. N. (1978). Intestinal disappearance and portal blood appearance of amino acids in sheep. Journal of Nutrition 108, 790803.
Terrill, T. H., Windham, W. R., Hoveland, C. S. & Amos, H. E. (1989). Forage preservation method influences on tannin concentration, intake and digestibility of sericea lespedeza by sheep. Agronomy Journal 81, 435439.
Terrill, T. H., Rowan, A. M., Douglas, G. B. & Barry, T. N. (1992 a). Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture 58, 321329.
Terrill, T. H., Douglas, G. B., Foote, A. G., Purchas, R. W., Wilson, G. F. & Barry, T. N. (1992 b). Effect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum coronarium) and perennial pasture. Journal of Agricultural Science, Cambridge 119, 265273.
Thomson, D. J., Beever, D. E., Harrison, D. G., Hill, I. W. & Osbourn, D. F. (1971). The digestion of dried lucerne (Medicago sativa L.) and dried sainfoin (Onobrychis viciifolia Scop.) by sheep. Proceedings of the Nutrition Society 30, 14A.
Ulyatt, M. J., Lancashire, J. A. & Jones, W. T. (1976). The nutritive value of legumes. Proceedings of the New Zealand Grassland Association 38, 107118.
Waghorn, G. C. & Jones, W. T. (1989). Bloat in cattle. 46. Potential of dock (Rumex obtusifolius) as an antibloat agent for cattle. New Zealand Journal of Agricultural Research 32, 227235.
Waghorn, G. C., John, A., Jones, W. T. & Shelton, I. D. (1987 a). Nutritive value of Lotus corniculatusL. containing low and medium concentrations of condensed tannins for sheep. Proceedings of the New Zealand Society of Animal Production 47, 2530.
Waghorn, G. C., Ulyatt, M. J., John, A. & Fisher, M. T. (1987 b). The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus L. British Journal of Nutrition 57, 115126.
Waghorn, G. C., Jones, W. T., Shelton, I. D. & McNabb, W. C. (1990). Condensed tannins and the nutritive value of herbage. Proceedings of the New Zealand Grasslands Association 51, 171176.
Waghorn, G. C., Shelton, I. D. & McNabb, W. C. (1994). Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep. 1. Non-nitrogenous aspects. Journal of Agricultural Science, Cambridge 123, 99107.
Wallace, R. J. & Cotta, M. A. (1988). Metabolism of nitrogen containing compounds. In The Rumen Microbial Ecosystem (Ed. Hobson, P. N.), pp. 217249. London: Elsevier Applied Science.
Windham, W. R., Petersen, J. C. & Terrill, T. H. (1990). Tannins as anti-quality factors in forage. In Microbial and Plant Opportunities to Improve Lignocellulose Utilization by Ruminants (Eds Akin, D. E., Ljungdahl, L. G., Wilson, J. R. & Harris, P. J.), pp. 127135. New York: Elsevier.


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