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Effects of age and species on agronomic performance, chemical composition and in vitro gas production of some tropical multi-purpose tree species

  • O. M. ARIGBEDE (a1) (a2), Z. L. TAN (a1), U. Y. ANELE (a2), Z. H. SUN (a1), S. X. TANG (a1), X. F. HAN (a1), C. S. ZHOU (a1) and B. ZENG (a1)...


Five tropical multi-purpose tree species (MPTS), Enterolobium cyclocarpum, Moringa oleifera, Millettia griffoniana, Pterocarpus santalinoides and Treculia Africana, and one exotic species (Leucaena leucocephala), which acted as the control, planted in a randomized complete block design, were selected to evaluate the potentials as feed supplements for herds in dry seasons of south-western Nigeria. Samples of the MPTS leaves were collected in 2005, 2006, 2007 and 2008, weighed, dried and milled for estimation of biomass production, chemical analysis and measurement of in vitro gas production. Results showed significant differences (P < 0·001) in the biomass production of the MPTS. E. cyclocarpum consistently recorded the highest biomass production, height and collar diameter throughout the experimental period. There were also differences (P ⩽ 0·001) in dry matter (DM), crude protein (CP), ether extract (EE) and ash contents of the MPTS across species and years after planting. M. oleifera recorded the highest CP content of 240 g/kg DM. Interactions were observed between species and year for neutral detergent fibre (NDFom), acid detergent fibre (ADFom) and lignin contents of the MPTS. M. griffoniana and M. oleifera had lower tannin contents, whereas E. cyclocarpum, P. santalinoides, T. africana and L. leucocephala had higher tannin contents. The values reported for mineral contents showed that their levels in the MPTS were adequate for ruminants and there were no differences in the contents of most minerals. In vitro gas production results showed that the potential gas production ‘b’ was highest in M. griffoniana and lowest in P. santalinoides and there were species and year interactions (P ⩽ 0·001) for volatile fatty acid profiles of the supernatant after 96 h incubation. The estimated organic matter digestibility (OMD) and metabolizable energy (ME) of the MPTS were generally high. The high biomass production, CP and low fibre contents, as well as in vitro fermentation characteristics found for the MPTS evaluated in the current study suggest that these are better alternatives in comparison with L. leucocephala. It can be concluded that the MPTS evaluated have potential as sources of feed supplements for ruminants in dry seasons, especially in south western Nigeria.


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Akkasaeng, R., Gutteridge, R. C. & Wanapat, M. (1989). Evaluation of trees and shrubs for forage and fuelwood in Northeast Thailand. International Tree Crops Journal 5, 209220.
Anele, U. Y., Arigbede, O. M., Olanite, J. A., Adekunle, I. O., Jolaosho, A. O., Onifade, O. S. & Oni, A. O. (2008). Early growth and seasonal chemical composition of three indigenous multipurpose tree species (MPTS) in Abeokuta, Nigeria. Agroforestry Systems 73, 8998.
AOAC (1990). Official Methods of Analysis, 15th edn. Washington, DC: AOAC.
Apori, S. O., Castro, E. B., Shand, W. J. & Orskov, E. R. (1998). Chemical composition, in-sacco degradation and in vitro gas production of some Ghanian browse plants. Animal Feed Science and Technology 76, 129137.
ARC (1984). Nutrient Requirements of Ruminant Livestock. Supplement No.1. Farnham, Surrey, UK: Commonwealth Agricultural Bureaux.
Arigbede, O. M., Anele, U. Y., Jolaosho, A. O., Olanite, J. A., Onifade, O. S. & Wahab, T. A. (2008). Chemical composition and in vitro gas production of African bread fruit (Treculia africana) var. Decne. Archivos de Zootecnia 57, 113121.
Arigbede, O. M. & Ekpenyon, T. E. (2004). Evaluation of three indigenous multi- purpose tree species for agroforestry purposes on alfisols in South Western Nigeria. Moor Journal of Agricultural Research 5, 108114.
Camacho, L. M., Rojo, R., Salem, A. Z. M., Provenza, F. D., Mendoza, G. D., Aviles, F. & Montanez-Valdez, O. D. (2010). Effect of season on chemical composition and in situ degradability in cows and in adapted and unadapted goats of three Mexican browse species. Animal Feed Science and Technology 155, 206212.
D'Mello, J. P. F. (1992). Chemical constraints to the use of tropical legumes in animal nutrition. Animal Feed Science and Technology 38, 237361.
Dalzell, S. A., Stewart, J. L., Tolera, A. & McNeill, D. M. (1998). Chemical composition of Leucaena and implications for forage quality. In ACIAR Proceedings of the Workshop Leucaena – Adaptation, Quality and Farming Systems, Hanoi, Vietnam, 9–14 February 1998 (Eds Shelton, H. M., Gutteridge, R. C., Mullen, B. F. & Bray, R. A.), pp. 227246. Canberra, Australia: ACIAR.
Jabbar, M. A., Larbi, A. & Reynolds, L. (1996). Alley Farming for Improving Small Ruminant Productivity in West Africa: ILRI's Experience. Socioeconomics & Policy Research Working Paper No. 20. Addis Ababa: International Livestock Research Institute.
Kang, B. T., Salako, F. K., Akobundu, I. O., Pleysier, J. L. & Chianu, J. N. (1997). Amelioration of a degraded Oxic Paleustalf by leguminous and natural fallows. Soil Use and Management 13, 130136.
Khanal, R. C. & Subba, D. B. (2001). Nutritional evaluation of leaves from some major fodder trees cultivated in the hills of Nepal. Animal Feed Science and Technology 92, 1732.
Lanyasunya, T. P., Wang, H. R., Kariuki, S. T., Kuria, D. M., Chek, A. L. & Mukisira, E. A. (2007). Effect of maturity on the mineral content of hairy vetch (Vicia villosa). Tropical and Subtropical Agroecosystems 7, 5358.
Larbi, A., Smith, J. W., Adekunle, I. O. & Kurdi, I. O. (1996). Studies on multipurpose fodder trees and shrubs in West Africa: variation in determinants of forage quality in Albizia and Paraserianthes species. Agroforestry Systems 33, 2939.
Ly, J., Samkol, P. & Preston, T. R. (2001). Nutritional evaluation of tropical leaves for pigs: pepsin/pancreatin digestibility of thirteen plant species. Livestock Research for Rural Development 13(5). Available online at (verified 29 November 2011).
Mahipala, M. B. P. K., Krebs, G. L., McCafferty, P., Dods, K. & Suriyagoda, B. M. L. D. B. (2009). Faecal indices predict organic matter digestibility, short chain fatty acid production and metabolizable energy content of browse-containing sheep diets. Animal Feed Science and Technology 154, 6875.
Makkar, H. P. S. (2003). Quantification of Tannins in Tree and Shrub Foliage. A Laboratory Manual. Dordrecht: Kluwer Academic Publishers.
Makkar, H. P. S., Blümmel, M., Borrowy, N. K. & Becker, K. (1993). Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Journal of the Science of Food and Agriculture 61, 161165.
Makkar, H. P. S., Singh, B. & Negi, S. S. (1989). Relationship of rumen degradability with microbial colonization, cell wall constituents and tannin levels in some tree leaves. Animal Production 49, 299303.
Malcolmson, G. H. (1988). Leucaena Establishment and Extension. Project Report Q0088007. Brisbane, Australia: Queensland Department of Primary Industries.
Mbugua, D. M., Kiruiro, E. M. & Pell, A. N. (2008). In vitro fermentation of intact and fractionated tropical herbaceous and tree legumes containing tannins and alkaloids. Animal Feed Science and Technology 146, 120.
McDowell, L. R. (1997). Minerals for Grazing Ruminants in Tropical Regions. Florida, USA: University of Florida.
McNabb, W. C., Waghorn, G. C., Barry, T. N. & Shelton, I. D. (1993). The effect of condensed tannins in Lotus pedunculatus on the digestion and metabolism of methionine, cystine and inorganic sulphur in sheep. British Journal of Nutrition 70, 647661.
Menke, K. H. & Steingass, H. (1988). Estimation of the energetic feed value from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28, 755.
Monforte-Briceno, G. E., Sandoval-Castro, C. A., Ramırez-Aviles, L. & Capetillo Leal, C. M. (2005). Defaunating capacity of tropical fodder trees: effects of polyethylene glycol and its relationship to in vitro gas production. Animal Feed Science and Technology 123, 313327.
Murro, J. K., Muhikambele, V. R. M. & Sarwatt, S. V. (2003). Moringa oleifera leaf meal can replace cottonseed cake in the concentrate mix fed with Rhodes grass (Chloris gayana) hay for growing sheep. Livestock Research for Rural Development 15(11). Available online at (verified 29 November 2011).
Odenyo, A. A., Osuji, P. O., Reed, J. D., Smith, A. H., Mackie, R. I., McSweeney, C. S. & Hanson, J. (2003). Acacia angustissima: its anti-nutrients constituents, toxicity and possible mechanisms to alleviate the toxicity – a short review. Agroforestry Systems 59, 141147.
Oni, A. O., Onwuka, C. F. I., Oduguwa, O. O., Onifade, O. S. & Arigbede, O. M. (2008). Utilization of citrus pulp based diets and Enterolobium cyclocarpum (JACQ.GRISEB) foliage by West African dwarf goats. Livestock Science 117, 184191.
Rajaguru, A. S. B. (1990). Shrubs and tree fodder used for livestock feeding in Sri Lanka. In Proceedings of the 2nd Regional Workshop on Multipurpose Tree Species (Ed. H. Gunasena, P. M.), pp. 8691. Bangkok, Thailand: Winrock International (F/FRED).
Sallam, S. M. A. (2005). Nutritive value assessment of the alternative feed resources by gas production and rumen fermentation in vitro . Research Journal of Agriculture and Biological Sciences 1, 200209.
Salunkhe, D. K., Chavan, J. K. & Kadam, S. S. (1990). Dietary Tannins: Consequences and Remedies. Boca Raton, FL: CRC Press Inc.
SAS (2002). User's Guide: Statistics. Version 9·1. Cary, NC: SAS Institute, Inc.
Satjapradja, O. & Sukandi, T. (1981). Agroforestry with red Calliandra (Calliandra calothyrsus). Indonesian Agriculture Research Development Journal 3, 8588.
Shelton, H. M., Lowry, J. B., Gutteridge, R. C., Bray, R. A. & Wildin, J. H. (1991). Sustaining productive pastures in the tropics. 7. Tree and shrub legumes in improved pastures. Tropical Grasslands 25, 119128.
Soliva, C. R., Zeleke, A. B., Clement, C., Hess, H. D., Fievez, V. & Kreuzer, M. (2008). In vitro screening of various tropical foliages, seeds, fruits and medicinal plants for low methane and high ammonia generating potentials in the rumen. Animal Feed Science and Technology 147, 5371.
Sun, Z. H., Liu, S. M., Tayo, G. O., Tang, S. X., Tan, Z. L., Lin, B., He, Z. X., Hang, X. F., Zhou, Z. S. & Wang, M. (2009). Effects of cellulase or lactic acid bacteria on silage fermentation and in vitro gas production of several morphological fractions of maize stover. Animal Feed Science and Technology 152, 219231.
Ty, H. X. (1996). Adaptability trials with Calliandra calothyrsus in Vietnam. In: Proceedings of an International Workshop on the Genus Calliandra, January 23–27, 1996, Bogor, Indonesia. Forest, Farm, and Community Tree Research Reports Special Issue, 1996 (Ed. Evans, D. O.), pp. 120126. Arkansas, USA: Winrock International, Morrilton.
Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833597.
Waghorn, G. C. (1990). Effect of condensed tannin on protein digestion and nutritive value of fresh herbage. Proceedings of Australian Society of Animal Production. 18, 412415.


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