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Effect of morpho-physiological traits on grain yield of sorghum grown under stress at different growth stages, and stability analysis

  • R. SANKARAPANDIAN (a1), S. AUDILAKSHMI (a2), V. SHARMA (a3), K. GANESAMURTHY (a4), H. S. TALWAR (a2) and J. V. PATIL (a2)...


Recent trends in climate change resulting in global warming and extreme dry spells during rainy seasons are having a negative impact on grain and fodder production in rain-fed crops in India. Understanding the mechanisms of drought tolerance at various growth stages will help in developing tolerant genotypes. Crosses were made between elite and drought-tolerant sorghums, and F2 and F3 progenies were evaluated for drought tolerance in multiple locations. Twenty-five F4/F5 derivatives along with drought-tolerant check plants (two high-yielding genotypes showing moderate drought tolerance: C43 (male parent of the commercial hybrid CSH 16, tolerant to drought) and CSV 17, (a pure line commercial cultivar released for drought-prone areas) were screened for drought tolerance under a factorial randomized block design with three replications during the rain-free months of April–June in 2007 and 2008 at Tamil Nadu Agricultural University, Kovilpatti, India. In each generation/year, four trials were conducted and water stress at different phases of crop growth, viz. vegetative, flowering and post-flowering (maturity), was imposed by withholding irrigation. Observations were recorded on grain and straw yields, plant height, number of roots, root length, leaf relative water content (LRWC), chlorophyll content and stomatal conductance under all treatments. The traits, grain yield, plant height, average root length and stomatal conductance showed significant mean sums of squares (SSs) for genotype × environment (G × E), suggesting that genotypes had significant differential response to the changing environments. Significant mean SSs due to G × E (linear) were obtained for straw yield, LRWC and chlorophyll content, indicating that the variability is partly genetic and partly influenced by environment. Grain yield was correlated with chlorophyll content (r = 0·43) at the vegetative stage, with number of roots (r = 0·49), LRWC (r = 0·51), chlorophyll content (r = 0·46) and stomatal conductance (r = −0·51) at the pre-flowering stage, and with LRWC (r = 0·50) and stomatal conductance (r = −0·40) at the post-flowering stage, under water stress. Partial least square (PLS) analysis showed that different traits were important for grain yield under water stress at different growth stages. Pyramiding the genes for the traits responsible for high grain yield under stress will help in developing stable genotypes at different stages of plant growth.


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Black, C. A. (1965). Methods of Soil Analysis, Vol. I. Madison, WI: American Society of Agronomy.
Borrell, A. K. & Hammer, G. L. (2000). Nitrogen dynamics and the physiological basis of stay-green in sorghum. Crop Science 40, 12951307.
Borrell, A. K., Hammer, G. L. & Douglas, A. C. L. (2000). Does maintaining green leaf area in sorghum improve yield under drought? Leaf growth and senescence. Crop Science 40, 10261037.
Blum, A. (2005). Drought resistance, water-use efficiency, and yield potential – are they compatible, dissonant, or mutually exclusive? Australian Journal of Agricultural Research 56, 11591168.
Davies, W. J. & Zhang, J. (1991). Root signals and the regulation of growth and development of plants in drying soil. Annual Review of Plant Physiology and Plant Molecular Biology 42, 5576.
Eastin, J. D. (1972). Photosynthesis and translocation in relation to plant development. In Sorghum in Seventies (Eds Rao, N. G. P. & House, L. R.), pp. 214246. New Delhi, India: Oxford and IBH Publishing Co.
Eberhart, S. A. & Russell, S. A. (1966). Stability parameters for comparing varieties. Crop Science 6, 3640.
Eitzinger, J., Orlandini, S., Stefanski, R. & Naylor, R. E. L. (2010). Climate change and agriculture: introductory editorial. Journal of Agricultural Science, Cambridge 148, 499500.
Farquhar, G. D. & Richards, R. A. (1984). Isotopic composition of plant carbon correlates with water-use efficiency of wheat genotypes. Australian Journal of Plant Physiology 11, 539552.
Finlay, K. W. & Wilkinson, G. N. (1963) Analysis of adaptation in plant breeding programme. Australian Journal of Agricultural Research 14, 742754.
González, A., Bermejo, V. & Gimeno, B. S. (2010). Effect of different physiological traits on grain yield in barley grown under irrigated and terminal water deficit conditions. Journal of Agricultural Science, Cambridge 148, 319328.
Jurs, P. C. (1990). Chemometrics and multivariate analysis in analytical chemistry. In Reviews in Computational Chemistry Vol. 1 (Eds Lipkowitz, K. B. & Boyd, D. B.), pp. 169212. New York: VCH Publishers.
Ludlow, M. M. (1980). Adaptive significance of stomatal responses to water stress. In Adaptation of Plants to Water and High Temperature Stress (Eds Turner, N. C. & Kramer, P. J.), pp. 123138. New York, USA: John Wiley.
Ludlow, M. M., Santamaria, J. M. & Fukai, S. (1990). Contribution of osmotic adjustment to grain yield in Sorghum bicolor (L.) Moench under water-limited conditions. II. Water stress after anthesis. Australian Journal of Agricultural Research 41, 6778.
Maiti, R. K. (1996). Root development and growth. In Sorghum Science (Ed. Maiti, R. K.), pp. 183212. New Delhi: Oxford and IBH Publishing Company Private Ltd.
Markhart, A. H. (1985). Comparative water relations of Phaseolus vulgaris L. and Phaseolus acutifolius Gray. Plant Physiology 77, 113117.
Mitra, J. (2001). Genetics and genetic improvement of drought resistance in crop plants. Current Science 80, 758763.
Mohammadi, R., Amri, A., Haghparast, R., Sadeghzadeh, D., Armion, M. & Ahmadi, M. M. (2009). Pattern analysis of genotype-by-environment interaction for grain yield in durum wheat. Journal of Agricultural Science, Cambridge 147, 537545.
Mutava, R. N., Prasad, P. V. V., Tuinstra, M. R., Kofoid, K. D. & Yu, J. (2011). Characterization of sorghum genotypes for traits related to drought tolerance. Field Crop Research 123, 1018.
Mutisya, J., Sitieney, J. K. & Gichuki, S. T. (2010). Phenotypic and physiological aspects related to drought tolerance in sorghum. African Crop Science Journal 18, 175182.
Patil, R. R. & Biradar, B. D. (2005). Heterosis studies for root and productivity traits in rabi sorghum [Sorghum bicolor (L.) Moench.]. Indian Journal of Genetics and Plant Breeding 65, 213214.
Rao, S. S., Seetharama, N., Kiran Kumar, K. A. & Vanderlip, R. L. (2004). Characterization of Sorghum Growth Stages. NRCS Bulletin Series No. 14. Rajendranagar, Hyderabad, India: National Research Centre for Sorghum.
Reddy, B. V. S., Rao, P., Deb, U. K., Stenhouse, J. W., Ramaiah, B. & Ortiz, R. (2004). Global sorghum genetic enhancement processes at ICRISAT. In Sorghum Genetic Enhancement: Research Process, Dissemination and Impacts (Eds Bantilian, M. C. S., Deb, U. K., Gowda, C. L. L., Reddy, B. V. S., Obilana, A. B. & Evenson, R. E.), pp. 65102. Patancheru, India: International Crops Research Institute for Semi-arid Tropics.
Rosenow, D. T., Ejeta, G., Clark, L. E., Gilbert, M. L., Henzell, R. G., Borrell, A. K. & Muchow, R. C. (1997). Breeding for pre- and post-flowering drought stress resistance in sorghum. In Proceedings of the International Conference on Genetic Improvement of Sorghum and Pearl Millet, 22–27 September 1996 (Ed. ICRISAT), pp. 400411. Lubbock, Texas: INTSRMIL and ICRISAT.
Sankarapandian, R. & Bangarusamy, U. (1995). Stability of sorghum genotypes for certain physiological characters and yield under water stress conditions. Crop Improvement 23, 6165.
Sankarapandian, R., Krishnadoss, N., Muppidathi, N. & Chidambaram, N. (1993). Variability studies in grain sorghum for certain physiological characters under water stress conditions. Crop Improvement 20, 4550.
Santamaria, J. M., Ludlow, M. M. & Fukai, S. (1990). Contribution of osmotic adjustment to grain yield in Sorghum bicolor (L.) Moench under water limited conditions. I. Water stress before anthesis. Australian Journal of Agricultural Research 41, 5165.
Sener, O., Arslan, M., Soysal, Y. & Erayman, M. (2009). Estimates of relative yield potential and genetic improvement of wheat cultivars in the Mediterranean region. Journal of Agricultural Science, Cambridge 147, 323332.
Sritharan, N. & Vijayalakshmi, C. (2008). Chlorophyll pigments, gas exchange parameters, membrane integrity and yield potential of rice genotypes under aerobic condition. Advances in Plant Science 21, 545548.
Subbarao, G. V., Johansen, C., Slinkard, A. E., Nageswara Rao, R. C., Saxena, N. P. & Chauhan, Y. S. (1995). Strategies for improving drought resistance in grain legumes. Critical Reviews in Plant Sciences 14, 469523.
Subudhi, P. K., Rosenow, D. T. & Nguyen, H. T. (2000). Quantitative trait loci for the stay green trait in sorghum (Sorghum bicolor (L.) Moench): consistency across genetic backgrounds and environments. Theoretical and Applied Genetics 101, 733741.
Talwar, H. S., Surwenshi, A. & Seetharama, N. (2009). Use of SPAD chlorophyll meter to screen sorghum (Sorghum bicolor L.) lines for postflowering drought tolerance. Indian Journal of Agricultural Sciences 79, 3539.
Tangpremsri, T., Fukai, S., Fischer, K. S. & Henzell, R. G. (1991). Genotypic variation in osmotic adjustment in grain sorghum. II. Relation with some growth attributes. Australian Journal of Agricultural Research 42, 759767.
Tuinstra, M. R., Grote, E. M., Goldsbrough, P. B. & Ejeta, G. (1997). Genetic analysis of post-flowering drought tolerance and components of grain development in Sorghum bicolor (L.) Moench. Molecular Breeding 3, 439448.
Vanderlip, R. L. & Reeves, H. E. (1972). Growth stages of sorghum. Agronomy Journal 64, 1316.
Weatherley, P. E. (1950). Studies in water relation of the cotton plant. I. The field measurement of water deficits in leaves. New Phytologist 49, 8197.
Wenkert, W. (1983). Water transport and balance within the plant: an overview. In Limitations to Efficient Water Use in Crop Plants (Eds Taylor, H. M., Jordan, W. R. & Sinclair, T. R.), pp. 137172. Madison, WI: American Society of Agronomy.
Wold, S. (1994). PLS for multivariate linear modeling. In QSAR: Chemometric Methods in Molecular Design: Methods and Principles in Medicinal Chemistry (Ed. van de Waterbeemd, H.), pp. 195218. Weinheim, Germany: Verlag-Chemie.
Xin, Z., Aiken, R. & Burke, J. (2009). Genetic diversity of transpiration efficiency in sorghum. Field Crops Research 111, 7480.
Xu, W., Rosenow, D. T. & Nguyen, H. T. (2000). Stay green trait in grain sorghum: relationship between visual rating and leaf chlorophyll concentration. Plant Breeding 119, 365367.
Zadoks, J. C., Chang, T. T. & Konzak, C. F. (1974). A decimal code for the growth stages of cereals. Weed Research 14, 415421.


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