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The effect of dicyandiamide addition to cattle slurry on soil gross nitrogen transformations at a grassland site in Northern Ireland

  • K. L. McGEOUGH (a1), C. MÜLLER (a2) (a3), R. J. LAUGHLIN (a1), C. J. WATSON (a1), M. ERNFORS (a4) (a5), E. CAHALAN (a2) (a4) and K. G. RICHARDS (a4)...


Many studies have shown the efficacy of the nitrification inhibitor dicyandiamide (DCD) in reducing nitrous oxide (N2O) emissions and nitrate (NO3 ) leaching. However, there is no information on the effect of DCD on gross soil N transformations under field conditions, which is key information if it is to be used as a mitigation strategy to reduce N losses. The current field study was conducted to determine the effect of DCD on ten gross nitrogen (N) transformations in soil following cattle slurry (CS) application to grassland in Northern Ireland on three occasions (June 2010, October 2010 and March 2011).

Ammonium (NH4 +) oxidation (ONH4) was the dominant process in total NO3 production (ONH4+ONrec (oxidation of recalcitrant organic N to NO3 )) following CS application, accounting for 0·894–0·949. Dicyandiamide inhibited total NO3 production from CS by 0·781, 0·696 and 0·807 in June 2010, October 2010 and March 2011, respectively. The lower inhibition level in October 2010 was thought to be due to the higher rainfall and soil moisture content in that month compared to the other application times. As DCD strongly inhibited NH4 + oxidation following CS application, it also decreased the rate of total NO3 consumption, since less NO3 was formed. The rates of mineralization from recalcitrant organic-N (MNrec) were higher than from labile organic-N (MNlab) on all occasions. The DCD significantly increased total mineralization (MNrec+MNlab) following CS application in June 2010 and March 2011, but had no significant effect in October 2010. In contrast, the rate of immobilization of labile organic-N (INH4_Nlab) was higher than from recalcitrant organic-N (INH4_Nrec) on all occasions, accounting for 0·878–0·976 of total NH4 + immobilization from CS. The DCD significantly increased total immobilization (INH4_Nrec+INH4_Nlab) when CS was applied in June 2010, but had no significant effect at other times of the year.

Dicyandiamide was shown to be a highly effective inhibitor of ammonium oxidation at this grassland site. Although there was evidence that it increased both NH4 + mineralization and immobilization following CS application, its effect on these processes was inconsistent. Further work is required to understand the reason for these inconsistent effects: future improvements in 15N tracer models may help.


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Bremner, J. M. & Mulvaney, C. S. (1982). Nitrogen – total. In Methods of Soil Analysis, Part 2: Chemical and Microbiological Properties (Eds Page, A. L., Miller, R. H. & Keeney, D. R.), pp. 595624. Madison, WI: American Society of Agronomy.
Briggs, G. G. (1975). Behaviour of nitrification inhibitor ‘N-Serve’ in broadcast and incorporated applications to soil. Journal of the Science of Food and Agriculture 26, 10831092.
Cameron, K. C., Di, H. J. & Moir, J. L. (2013). Nitrogen losses from the soil/plant system: a review. Annals of Applied Biology 162, 145173.
Cooper, J. E. (1975). Nitrification in soils incubated with pig slurry. Soil Biology and Biochemistry 7, 119124.
Dale, A. J., Ferris, C. P., Frost, J. P., Mayne, C. S. & Kilpatrick, D. J. (2013). The effect of applying cattle slurry using the trailing-shoe technique on dairy cow and sward performance in a rotational grazing system. Grass and Forage Science 68, 138150.
DEFRA (2010). Fertiliser Manual (RB209). Norwich, UK: The Stationery Office.
Dennis, S. J., Cameron, K. C., Di, H. J., Moir, J. L., Staples, V., Sills, P. & Richards, K. G. (2012). Reducing nitrate losses from simulated grazing on grassland lysimeters in Ireland using a nitrification inhibitor (dicyandiamide). Biology and Environment: Proceedings of the Royal Irish Academy 112B, 7989.
Di, H. J. & Cameron, K. C. (2002). Nitrate leaching in temperate agroecosystems: sources, factors and mitigating strategies. Nutrient Cycling in Agroecosystems 64, 237256.
Di, H. J. & Cameron, K. C. (2003). Mitigation of nitrous oxide emissions in spray-irrigated grazed grassland by treating the soil with dicyandiamide, a nitrification inhibitor. Soil Use and Management 19, 284290.
Di, H. J. & Cameron, K. C. (2004). Effects of temperature and application rate of a nitrification inhibitor, dicyandiamide (DCD), on nitrification rate and microbial biomass in a grazed pasture soil. Australian Journal of Soil Research 42, 927932.
Di, H. J. & Cameron, K. C. (2005). Reducing environmental impacts of agriculture by using a fine particle suspension nitrification inhibitor to decrease nitrate leaching from grazed pastures. Agriculture, Ecosystems and the Environment 109, 202212.
Di, H. J. & Cameron, K. C. (2006). Nitrous oxide emissions from two dairy pasture soils as affected by different rates of a fine particle suspension nitrification inhibitor, dicyandiamide. Biology and Fertility of Soils 42, 472480.
Flowers, T. H. & O'Callaghan, J. R. (1983). Nitrification in soils incubated with pig slurry or ammonium sulphate. Soil Biology and Biochemistry 15, 337342.
Hendrickson, L. L. & Keeney, D. R. (1979). Effect of some physical and chemical factors on the rate of hydrolysis of nitrapyrin (n-serve). Soil Biology and Biochemistry 11, 4750.
Inselsbacher, E., Wanek, W., Strauss, J., Zechmeister-Boltenstern, S. & Müller, C. (2013). A novel 15N tracer model reveals: plant nitrate uptake governs nitrogen transformation rates in agricultural soils. Soil Biology and Biochemistry 57, 301310.
Juma, N. G. & Paul, E. A. (1983). Effect of a nitrification inhibitor on N immobilization and release of 15N from non-exchangeable ammonium and microbial biomass. Canadian Journal of Soil Science 63, 167175.
Keeney, D. R. (1986). Inhibition of nitrification in soils. In Nitrification (Ed. Prosser, J. I.), pp. 99115. Oxford, UK: IRL Press.
Kelliher, F. M., Clough, T. J., Clark, H., Rys, G. & Sedcole, J. R. (2008). The temperature dependence of dicyandiamide (DCD) degradation in soils: a data synthesis. Soil Biology and Biochemistry 40, 18781882.
Laughlin, R. J., Stevens, R. J. & Zhuo, S. (1997). Determining nitrogen-15 in ammonium by producing nitrous oxide. Soil Science Society of America Journal 61, 462465.
Monaghan, R. M., Smith, L. C. & Ledgard, S. F. (2009). The effectiveness of a granular formulation of dicyandiamide (DCD) in limiting nitrate leaching from a grazed dairy pasture. New Zealand Journal of Agricultural Research 52, 145149.
Müller, C., Stevens, R. J. & Laughlin, R. J. (2003). Evidence of carbon stimulated N transformations in grassland soil after slurry application. Soil Biology and Biochemistry 35, 285293.
Müller, C., Rütting, T., Kattge, J., Laughlin, R. J. & Stevens, R. J. (2007). Estimation of parameters in complex 15N tracing models by Monte Carlo sampling. Soil Biology and Biochemistry 39, 715726.
Müller, C., Laughlin, R. J., Christie, P. & Watson, C. J. (2011). Effects of repeated fertilizer and cattle slurry applications over 38 years on N dynamics in a temperate grassland soil. Soil Biology and Biochemistry 43, 13621371.
Norman, R. J. & Wells, B. R. (1989). Effect of dicyandiamide on the form and recovery of 15N-labeled urea in the delayed flood soil system. Communications in Soil Science and Plant Analysis 20, 20792089.
Oenema, O., Wrage, N., Velthof, G. L., van Groenigen, J. W., Dolfing, J. & Kuikman, P. J. (2005). Trends in global nitrous oxide emissions from animal production systems. Nutrient Cycling in Agroecosystems 72, 5165.
Oenema, O., Oudendag, D. & Velthof, G. L. (2007). Nutrient losses from manure management in the European Union. Livestock Science 112, 261272.
Pasda, G., Hähndel, R. & Zerulla, W. (2001). Effect of fertilizers with the new nitrification inhibitor DMPP (3,4-dimethylpyrazole phosphate) on yield and quality of agricultural and horticultural crops. Biology and Fertility of Soils 34, 8597.
Puttanna, K., Nanje Gowda, N. M. & Prakasa Rao, E. V. S. (1999). Effect of concentration, temperature, moisture, liming and organic matter on the efficacy of the nitrification inhibitors benzotriazole, o-nitrophenol, m-nitroaniline and dicyandiamide. Nutrient Cycling in Agroecosystems 54, 251257.
Salsac, L., Chaillou, S., Morot-Gaudry, J. F., Lesaint, C. & Jolivet, E. (1987). Nitrate and ammonium nutrition in plants. Plant Physiology and Biochemistry 25, 805812.
Shepherd, M., Wyatt, J. & Welten, B. (2012). Effect of soil type and rainfall on dicyandiamde concentrations in drainage from lysimeters. Australian journal of Soil Research 50, 6775.
Singh, J., Saggar, S., Giltrap, D. L. & Bolan, N. S. (2008). Decompostion of dicyandiamide (DCD) in three contrasting soils and its effect on nitrous oxide emission, soil respiratory activity, and microbial biomass – an incubation study. Australian Journal of Soil Research 46, 517525.
Smith, L. C., de Klein, C. A. M. & Catto, W. D. (2008). Effect of dicyandiamide applied in a granular form on nitrous oxide emissions from a grazed dairy pasture in Southland, New Zealand. New Zealand Journal of Agricultural Research 51, 387396.
Sørensen, P. (1998). Carbon mineralization, nitrogen immobilization and pH change in soil after adding volatile fatty acids. European Journal of Soil Science 49, 457462.
Stevens, R. J. & Laughlin, R. J. (1994). Determining Nitrogen-15 in nitrite or nitrate by producing nitrous oxide. Soil Science Society of America Journal 58, 11081116.
Stevens, R. J. & Laughlin, R. L. (1997). The impact of cattle slurries and their management on ammonia and nitrous oxide emissions from grassland. In Gaseous Nitrogen Emissions from Grassland (Eds Jarvis, S. C. & Pain, B. F.), pp. 233256. New York: CAB International.
Stevens, R. J. & Laughlin, R. L. (2001). Cattle slurry affects nitrous oxide and dinitrogen emissions from fertilizer nitrate. Soil Science Society of America Journal 65, 13071314.
Stevens, R. J., Laughlin, R. J., Burns, L. C., Arah, J. R. M. & Hood, R. C. (1997). Measuring the contributions of nitrification and denitrification to the flux of nitrous oxide from soil. Soil Biology and Biochemistry 29, 139151.
Subbarao, G. V., Sahrawat, K. L., Nakahara, K., Ishikawa, T., Kishii, M., Rao, I. M., Hash, C. T., George, T. S., Srinivasa Rao, P., Nardi, P., Bonnett, D., Berry, W., Suenaga, K. & Lata, J. C. (2012). Biological nitrification inhibition – a novel strategy to regulate nitrification in agricultural systems. Advances in Agronomy 114, 249302.
Thomson, C. J., Marschner, H. & Römheld, V. (1993). Effect of nitrogen fertilizer form on pH of the bulk soil and rhizosphere, and on the growth, phosphorus, and micronutrient uptake of bean. Journal of Plant Nutrition 16, 493506.
Williamson, J. C., Taylor, M. D., Torrens, R. S. & Vojvodic-Vukovic, M. (1998). Reducing nitrogen leaching from dairy farm effluent-irrigated pasture using dicyandiamide: a lysimeter study. Agriculture, Ecosystems and Environment 69, 8188.
Zerulla, W., Barth, T., Dressel, J., Erhardt, K., von Locquenghien, K. H., Pasda, G., Rädle, M. & Wissemeier, A. H. (2001). 3,4-Dimethylpyrazole phosphate (DMPP) – a new nitrification inhibitor for agriculture and horticulture. Biology and Fertility of Soils 34, 7984.
Zhang, H. J., Wu, Z. J. & Zhou, Q. X. (2004). Dicyandiamide sorption-desorption behaviour on soils and peat humus. Pedosphere 14, 395399.


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