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Developmental changes in the biochemical composition of muscle from ‘splayleg’ piglets with special reference to DNA synthesis

Published online by Cambridge University Press:  27 March 2009

Linda J. Farmer ,
W. S. Mackie  and
P. J. Ritchie
Linda J. Farmer
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
W. S. Mackie
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
P. J. Ritchie
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
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Summary

DNA, RNA and protein concentrations, cathepsin D activity and tritiated thymidine incorporation into DNA were measured in selected muscles from newborn to 7-day-old ‘splayleg’ piglets. The results indicated that the pattern of development differed considerably from that observed in muscle from normal piglets, and the range of results was greater both within and between affected animals. The most striking difference between muscle from normal and ‘splayleg’ piglets was in the incorporation of tritiated thymidine, which was lower in the latter from 3 to 6 days of age, indicating that the number of cells undergoing mitosis may have been reduced. It is suggested that this could have a severe effect on future muscle growth especially if satellite cells are affected.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 97 , Issue 3 , December 1981 , pp. 569 - 579
Copyright
Copyright © Cambridge University Press 1981

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