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A comparison of sample preparation and calibration techniques for the estimation of nitrogen, oil and glucosinolate content of rapeseed by near infrared spectroscopy

Published online by Cambridge University Press:  27 March 2009

Carol Starr
Affiliation:
Plant Breeding Institute, Maria Lane, Trumpington, Cambridge, 0B2 2LQ
Janet Suttle
Affiliation:
Plant Breeding Institute, Maria Lane, Trumpington, Cambridge, 0B2 2LQ
A. G. Morgan
Affiliation:
Plant Breeding Institute, Maria Lane, Trumpington, Cambridge, 0B2 2LQ
D. B. Smith
Affiliation:
Plant Breeding Institute, Maria Lane, Trumpington, Cambridge, 0B2 2LQ

Summary

Predictions of nitrogen, oil and glucosinolate concentration in rapeseed samples were made by near infrared reflectance analysis after various grinding treatments. Also examined were the effects of normalizing reflectance data and the possible advantage of using all combinations of two and three wavelengths in the calibration regression analysis over forward stepwise regression. The main conclusion was that drying the samples prior to a controlled grinding treatment gave the best results, although acceptable results for selection purposes could be obtained using whole seeds to predict nitrogen and oil. None of the treatments of the seed or reflectance data allowed acceptable prediction of glucosinolate content.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1985

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