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The calibration of a photo-electric absorptiometer for the rapid estimation of counts of spermatozoa in bull semen

Published online by Cambridge University Press:  27 March 2009

C. P. Cox  and
D. R. Melrose
C. P. Cox
Affiliation:
National Institute for Research in Dairying, University of Reading, and Reading Cattle Breeding Centre, Ministry of Agriculture and Fisheries
D. R. Melrose
Affiliation:
National Institute for Research in Dairying, University of Reading, and Reading Cattle Breeding Centre, Ministry of Agriculture and Fisheries
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Extract

1. An EEL mains-operated colorimeter has been calibrated to obtain rapid estimates of the concentration of spermatozoa in bull semen.

2. A general linear calibration relationship has been calculated giving h, the estimated concentration of spermatozoa per ml. of the original semen, as

where d is the dilution factor and Pd is the absorptiometer reading.

3. Under the circumstances of the experiments described, the accuracy of estimates from the above relation was about 1%, and it is considered that the instrument may be effectively used in semen determinations for artificial insemination and in most cases where a rapid determination is required.

4. Attention is drawn to the desirability of using standards and occasional check calibrations, and to the possibility that in routine use results may be slightly less accurate than those given above, which were obtained with laboratory determinations.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 43 , Issue 3 , July 1953 , pp. 375 - 379
Copyright
Copyright © Cambridge University Press 1953

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References

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