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Apparent digestibility, rumen fermentation and nitrogen balance in Tibetan and fine-wool sheep offered forage-concentrate diets differing in nitrogen concentration

  • J. W. ZHOU (a1) (a2), J. D. MI (a2) (a3), A. A. DEGEN (a4), X. S. GUO (a2) (a3), H. C. WANG (a1) (a2), L. M. DING (a2) (a3), Q. QIU (a3) and R. J. LONG (a1) (a2) (a3)...

Summary

A comparative study of the effect of dietary nitrogen (N) content [Low: 11·0; Medium-Low (MLow): 16·7; Medium-High (Mhigh): 23·1; High: 29·2 N g/kg dry matter (DM)] on apparent digestibilities, rumen fermentation and N balance was conducted in coarse wool Tibetan sheep and Gansu Alpine fine-wool sheep at Wushaoling in the northeast of the Qinghai-Tibetan Plateau. It was hypothesized that responses would differ between breeds and that responses would favour Tibetan over fine-wool sheep at low N intakes. Eight wethers [four Tibetan sheep and four fine-wool sheep, 20–24 months old; body weight ± standard deviation was 52 ± 3·2 kg] were used in two concurrent 4 × 4 Latin square designs. Dry matter, organic matter, neutral detergent fibre and acid detergent fibre digestibilities were higher in Tibetan than fine-wool sheep when fed the Low, MLow and High N diets while N retention was higher when the animals were fed the Low and MLow N diets. Tibetan sheep had a higher rumen pH than fine-wool sheep; however, total volatile fatty acids were similar between breeds. Molar proportions of acetate were higher but propionate and butyrate lower in Tibetan than fine-wool sheep. In addition, Tibetan sheep had higher concentrations of ruminal free amino acid-N and soluble protein-N than fine-wool sheep. Plasma and saliva urea-N concentrations were higher in Tibetan than fine-wool sheep when supplied with the Low N diet. It was concluded that Tibetan sheep were better able to cope with low N feed than fine-wool sheep because of the higher N retention and higher DM and fibre digestibilities with Low and MLow diets.

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Corresponding author

*To whom all correspondence should be addressed. Email: longrj@lzu.edu.cn

References

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Agle, M., Hristov, A. N., Zaman, S., Schneider, C., Ndegwa, P. M. & Vaddella, V. K. (2010). Effect of dietary concentrate on rumen fermentation, digestibility and nitrogen losses in dairy cows. Journal of Dairy Science 93, 42114222.
AOAC (1990). Official Methods of Analysis. 15th edn.Arlington, VA: Association of Official Analytical Chemists.
Bach, A., Yoon, I. K., Stern, M. D., Jung, H. G. & Chester-Jones, H. (1999). Effects of type of carbohydrate supplementation to lush pasture on microbial fermentation in continuous culture. Journal of Dairy Science 82, 153160.
Broderick, G. A. (2003). Effects of varying dietary protein and energy levels on the production of lactating dairy cows. Journal of Dairy Science 86, 13701381.
Cantalapiedra-Hijar, G., Yáñez-Ruiz, D. R., Martín-García, A. I. & Molina-Alcaide, E. (2009). Effects of forage: concentrate ratio and forage type on apparent digestibility, ruminal fermentation, and microbial growth in goats. Journal of Animal Science 87, 622631.
Cao, Y., Takahashi, T. & Horiguchi, K. (2009). Effects of addition of food by-products on the fermentation quality of a total mixed ration with whole crop rice and its digestibility, preference, and rumen fermentation in sheep. Animal Feed Science and Technology 151, 111.
Carro, M. D., Cantalapiedra-Hijar, G., Ranilla, M. J. & Molina-Alcaide, E. (2012). Urinary excretion of purine derivatives, microbial protein synthesis, nitrogen use, and ruminal fermentation in sheep and goats fed diets of different quality. Journal of Animal Science 90, 39633972.
Cheng, P. (1984). Livestock Breeds of China. Animal Production and Health Paper 46 (E, F, S,). Rome: FAO.
Dan, R. F., Long, R. J., Zhang, H. T., Zhang, X. & Ding, X. Z. (2009). Analysis of rumen bacterial flora of Tibetan sheep with seasonal shift. Chinese Journal of Animal Nutrition 21, 798802.
De Barbieri, I., Hegarty, R. S., Oddy, V. H., Barnett, M. C., LI, L. & Nolan, J. V. (2014). Sheep of divergent genetic merit for wool growth do not differ in digesta kinetics while on restricted intakes. Animal Production Science 54, 12431247.
Erwin, E. S., Marco, G. J. & Emery, E. (1961). Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. Journal of Dairy Science 44, 17681774.
Felix, T. L., Murphy, T. A. & Loerch, S. C. (2012). Effects of dietary inclusion and NaOH treatment of dried distillers grain with solubles on ruminal metabolism of feedlot cattle. Journal of Animal Science 90, 49514961.
Fimbres, H., Kawas, J. R., Hernández-Vidal, G., Picón-Rubio, J. F. & Lu, C. D. (2002). Nutrient intake, digestibility, mastication and ruminal fermentation of lambs fed finishing ration with various forage levels. Small Ruminant Research 43, 275281.
Fitzsimons, C., Kenny, D. A., Deighton, M. H., Fahey, A. G. & McGee, M. (2013). Methane emissions, body composition, and rumen fermentation traits of beef heifers differing in residual feed intake. Journal of Animal Science 91, 57895800.
Fu, C. J., Felton, E. E., Lehmkuhler, J. W. & Kerley, M. S. (2001). Ruminal peptide concentration required to optimize microbial growth and efficiency. Journal of Animal Science 79, 13051312.
Grant, R. J. & Mertens, D. R. (1992). Influence of buffer pH and raw corn starch addition on in vitro fibre digestion kinetics. Journal of Dairy Science 75, 27622768.
Hervás, G., Ranilla, M. J., Mantecón, A. R., Tejido, M. L. & Frutos, P. (2005). Comparison of sheep and red deer rumen fluids for assessing nutritive value of ruminant feedstuffs. Journal of the Science of Food and Agriculture 85, 24952502.
Hoffman, P. C., Esser, N. M., Bauman, L. M., Denzine, S. L., Engstrom, M. & Chester-Jones, H. (2001). Short communication: effect of dietary protein on growth and nitrogen balance of Holstein heifers. Journal of Dairy Science 84, 843847.
Hristov, A. & Broderick, G. A. (1994). In vitro determination of ruminal protein degradability using [15N] ammonia to correct for microbial nitrogen uptake. Journal of Animal Science 72, 13441354.
Hristov, A. N., Ivan, M., Rode, L. M. & McAllister, T. A. (2001). Fermentation characteristics and ruminal ciliate protozoal populations in cattle fed medium- or high-concentrate barley-based diets. Journal of Animal Science 79, 515524.
Huyen, N. T., Wanapat, M. & Navanukraw, C. (2012). Effect of Mulberry leaf pellet (MUP) supplementation on rumen fermentation and nutrient digestibility in beef cattle fed on rice straw-based diets. Animal Feed Science and Technology 175, 815.
Ipharraguerre, I. R., Clark, J. H. & Freeman, D. E. (2005). Varying protein and starch in the diet of dairy cows. 1. Effect on ruminal fermentation and intestinal supply of nutrients. Journal of Dairy Science 88, 25372555.
Kayouli, C., Jouany, J. P., Demeyer, D. I., Ali-Ali, Taoueb H. & Dardillat, C. (1993). Comparative studies on the degradation and mean retention time of solid and liquid phases in the forestomachs of dromedaries and sheep fed on low-quality roughages from Tunisia. Animal Feed Science and Technology 40, 343355.
Keady, T. W. J. & Mayne, C. S. (2001). The effects of concentrate energy source on feed intake and rumen fermentation parameters of dairy cows offered a range of grass silages. Animal Feed Science and Technology 90, 117129.
Kennedy, P. M. & Milligan, L. P. (1978). Transfer of urea from the blood to the rumen of sheep. British Journal of Nutrition 40, 149154.
Kennedy, P. M. & Milligan, L. P. (1980). The degradation and utilization of endogenous urea in the gastrointestinal tract of ruminants: a review. Canadian Journal of Animal Science 60, 205221.
Leng, R. A. & Nolan, J. V. (1984). Nitrogen metabolism in the rumen. Journal of Dairy Science 67, 10721089.
Li, W. (2011). Industry conditions, development direction and countermeasures of Gansu Alpine fine-wool sheep. Journal of Domestic Animal Ecology 32, 8083.
Li, L., Oddy, V. H. & Nolan, J. V. (2008). Whole-body protein metabolism and energy expenditure in sheep selected for divergent wool production when fed above or below maintenance. Australian Journal of Australian Agriculture 48, 657665.
Long, R. J. (2007). Functions of ecosystem in the Tibetan grassland. Chinese Science & Technology Review 25, 2628.
Long, R. J. & Ma, Y. S. (1996). Qinghai's yak production system. In Conservation and Management of Yak Genetic Diversity: Proceedings of a Workshop in ICIMOD, 29–31 October 1996, Kathmandu, Nepal (Eds Miller, D. J., Craig, S. R. & Rana, G. M.), pp. 105115. Kathmandu, Nepal: ICIMOD.
Long, R. J., Dong, S. K., Hu, Z. Z., Shi, J. J., Dong, Q. M. & Han, X. T. (2004). Digestibility, nutrient balance and urinary purine derivative excretion in dry yak cows fed oat hay at different levels of intake. Livestock Production Science 88, 2732.
Long, R. J., Dong, S. K., Wei, X. H. & Pu, X. P. (2005). The effect of supplementary feeds on the bodyweight of yaks in cold season. Livestock Production Science 93, 197204.
Long, R. J., Ding, L. M., Shang, Z. H. & Guo, X. H. (2008). The yak grazing system on the Qinghai-Tibetan Plateau and its status. Rangeland Journal 30, 241246.
MacRae, J. C., Milne, J. A., Wilson, S. & Spence, A. M. (1979). Nitrogen digestion in sheep given poor-quality indigenous hill herbages. British Journal of Nutrition 42, 525534.
Marini, J. C. & Van Amburgh, M. E. (2003). Nitrogen metabolism and recycling in Holstein heifers. Journal of Animal Science 81, 545552.
Mehrez, A. Z., Ørskov, E. R. & McDonald, I. (1977). Rates of rumen fermentation in relation to ammonia concentration. British Journal of Nutrition 38, 437443.
Molina-Alcaide, E., Martín García, A. I. & Aguilera, J. F. (2000). A comparative study of nutrient digestibility, kinetics of degradation and passage and rumen fermentation pattern in goats and sheep offered good-quality diets. Livestock Production Science 64, 215223.
Norton, B. W., Murray, R. M., Entwistle, K. W., Nolan, J. V., Ball, F. M. & Leng, R. A. (1978). The nitrogen metabolism of sheep consuming Flinders grass (Iseilema spp.), Mitchell grass (Astrebla spp.) and mixed native pasture. Australian Journal of Agricultural Research 29, 595603.
NRC (1985). Nutrient Requirements of Sheep, revised6th edn, Washington, D.C.: National Academy Press.
Oddy, V. H. (1999). Protein metabolism and nutrition in farm animals: an overview. In Proceedings of the 8th International Symposium on Protein Metabolism and Nutrition (Eds Lobley, G. E., White, A. & Macrae, J. C.), pp. 723. EAAP Publication No. 96. Wageningen, The Netherlands: Wageningen Academic Publishers.
Oosta, G. M., Mathewson, N. S. & Catravas, G. N. (1978). Optimization of Folin-Ciocalteu reagent concentration in an automated Lowry protein assay. Analytical Biochemistry 89, 3134.
Perdok, H. & Leng, R. A. (1989). Rumen ammonia requirements for efficient digestion and intake of straw by cattle. In The Roles of Protozoa and Fungi in Ruminant Digestion (Eds Nolan, J. V., Leng, R. A. & Demeyer, D. J.), pp. 291293. Armidale, Australia: Penambul Books.
Preston, T. R. & Leng, R. A. (1987). Matching Ruminant Production Systems with Available Resources in the Tropics and Sub-tropics. Armidale, Australia: Penambul Books.
Reynolds, C. K. & Kristensen, N. B. (2008). Nitrogen recycling through the gut and the nitrogen economy of ruminants: an asynchronous symbiosis. Journal of Animal Science 86 (Supplement), E293E305.
Satter, L. D. & Slyter, L. L. (1974). Effect of ammonia concentration on rumen microbial protein production in vitro. British Journal of Nutrition 32, 199208.
Silanikove, N., Tagari, H. & Shkolnik, A. (1993). Comparison of rate of passage, fermentation rate and efficiency of digestion of high fiber diet in desert Bedouin goats compared to Swiss Saanan goats. Small Ruminant Research 12, 4560.
Van Soest, P. J. (1994). Nutritional Ecology of the Rumen, 2nd edn.Ithaca, NY: Cornell University Press.
Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833597.
Varady, J., Tashenov, K. T., Boda, K., Fejes, J. & Kosta, K. (1979). Endogenous urea secretion into the sheep gastrointestinal tract. Physiologia Bohemoslovaca 28, 551559.
Wanapat, M. & Pimpa, O. (1999). Effect of ruminal NH3-N levels on ruminal fermentation, purine derivative, digestibility and rice straw intake in swamp buffaloes. Asian-Australasian Journal of Animal Science 12, 904907.
Wang, T. X. (2012). Breeding status and perspective of Gansu Alpine Merino. Journal of Animal Science and Veterinary Medicine 31, 4650.
Wiener, G., Han, J. L. & Long, R. J. (2003). The Yak. 2nd edn.Bangkok, Thailand: RAP Publication.
Xin, G. S., Long, R. J., Guo, X. S., Irvine, J., Ding, L. M., Ding, L. L. & Shang, Z. H. (2011). Blood mineral status of grazing Tibetan sheep in Northeast of the Qinghai-Tibetan Plateau. Livestock Science 136, 102107.
Xiong, B. H., Pang, Z. H. & Luo, Q. Y. (2009). Introduction of tables of feed composition and nutritive values in China (20th edition). Chinese Feed 21, 3035.
Zanton, G. I. & Heinrichs, A. J. (2009). Digestion and nitrogen utilization in dairy heifers limit-fed a low or high forage ration at four levels of nitrogen intake. Journal of Dairy Science 92, 20782094.
Zhang, Y., Zhou, J. W., Guo, X. S., Cui, G. X., Ding, L. M., Wang, H. C., Li, L. W. & Long, R. J. (2012). Influences of dietary nitrogen and non-fibre carbohydrate levels on apparent digestibility, rumen fermentation and nitrogen utilization in growing yaks fed low quality forage based-diet. Livestock Science 147, 139147.
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