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The effect of Rhizobium inoculation on white clover in improved hill soils in the United Kingdom

Published online by Cambridge University Press:  27 March 2009

P. Newbould ,
A. J. Holding ,
G. J. Davies ,
Anne Rangeley ,
G. J. F. Copeman ,
A. Davies ,
J. Frame ,
A. Haystead ,
J. B. D. Herriott  and
J. C. Holmes
...Show all authors
P. Newbould
Affiliation:
Hill Farming Research Organisation, Bush Estate, Penicuik, Midlothian, EH26 OPY.
A. J. Holding
Affiliation:
The Edinburgh School of Agriculture, Microbiology Department, King's Buildings, West Mains Road, Edinburgh, EH9 3JG.
G. J. Davies
Affiliation:
Agricultural Research Council Unit of Statistics, University of Edinburgh, James Clerk Maxwell Building, Mayfield Road, Edinburgh, EHd 3JZ.
Anne Rangeley
Affiliation:
Hill Farming Research Organisation, Bush Estate, Penicuik, Midlothian, EH26 OPY.
G. J. F. Copeman
Affiliation:
North of Scotland College of Agriculture, 581 King Street, Aberdeen, AB9 1 UD.
A. Davies
Affiliation:
Welsh Plant Breeding Station, Plas Gogerddan, Near Aberystwyth, SY23 3EB.
J. Frame
Affiliation:
West of Scotland Agricultural College, Auchincruive, Ayr, KA6 5HW.
A. Haystead
Affiliation:
Hill Farming Research Organisation, Bush Estate, Penicuik, Midlothian, EH26 OPY.
J. B. D. Herriott
Affiliation:
East of Scotland College of Agriculture, King's Buildings, West Mains Road, Edinburgh, EH9 3JG.
J. C. Holmes
Affiliation:
East of Scotland College of Agriculture, King's Buildings, West Mains Road, Edinburgh, EH9 3JG.
J. F. Lowe
Affiliation:
The Edinburgh School of Agriculture, Microbiology Department, King's Buildings, West Mains Road, Edinburgh, EH9 3JG.
J. W. G. Parker
Affiliation:
Ministry of Agriculture, Fisheries and Food, Agricultural Development and Advisory Service, Great House Experimental Husbandry Farm, Helmshore, Rossendale, Lanes, BB4 4AJ.
H. A. Waterson
Affiliation:
West of Scotland Agricultural College, Auchincruive, Ayr, KA6 5HW.
J. Wildig
Affiliation:
Ministry of Agriculture, Fisheries and Food, Agricultural Development and Advisory Service, Pwllpeiran Experimental Husbandry Farm, Cumystwyth, Aberystwyth, SY23 4AB.
J. P. Wray
Affiliation:
Ministry of Agriculture, Fisheries and Food, Agricultural Development and Advisory Service, Redesdale Experimental Hiisbandry Farm, Rochester, Olterburn, Newcastle-upon-Tyne, NE19 ISD.
D. Younie
Affiliation:
North of Scotland College of Agriculture, 581 King Street, Aberdeen, AB9 1 UD.
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Summary

The benefits to establishment and growth of white clover cvs Aberystwyth S.184 and Grasslands Huia of inoculation with three strains of Rhizobium trifolii, using the peat or liquid inoculum techniques, were investigated during 1975–8 on improved hill soils ranging from brown earth through dry and wet peaty podzol to deep peat.

Inoculation induced positive response in either number of seedlings, plant cover or dry-matter production in 18 out of 139 comparisons, had no effect in 118 and produced a negative response in three. Most of the positive responses to inoculation were at sites with wet peaty podzol or deep peat soils but of the five sites where increase in clover D.M. production was found in the first harvest year one was a brown earth. The positive agronomic responses occurred only when the proportion of plants with nodules was high and where a substantial proportion ( > 50%) of the latter contained introduced Rhizobium strains at least in the year of sowing. The three negative responses were in numbers of seedlings on one brown earth and two dry peaty podzol soils and with the Huia cultivar only. Despite lack of statistical significance at individual sites the dominant overall trend was for inoculation to enhance seedling establishment and the early growtli of white clover in all soil types.

On one brown earth and one dry peaty podzol soil there was some evidence that spraying the Rhizobium on to emerging white clover seedlings was more beneficial, atleast in microbiological terms, than the customary peat inoculum procedure.

The incorporation of even a small amount of nitrogen (30 kg/ha) into the seed bed at the time of sowing adversely affected germination, establishment and growth of white clover in some soils. Sometimes the effects of this nitrogen persisted into the first harvest year.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 99 , Issue 3 , December 1982 , pp. 591 - 610
Copyright
Copyright © Cambridge University Press 1982

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References

Agricultural Research Council (1975). The inoculation of white clover seeds with effective strains of Rhizobium trifolii for sowing in hill soils. Agricultural Research Council Annual Report, 1973–74, p. 12.Google Scholar
Agricultural Research Council (1978). Establishment of white clover in the hills. Agricultural Research Council Annual Report, 1977–78, pp. 14–15.Google Scholar
Allen, S. E. (ed.) (1974). Chemical Analysis of Ecological Materials. Oxford: Blackwell.Google Scholar
Bastiman, B. (1969). Response to clover in upland swards. Experimental Husbandry 18, 38–43.Google Scholar
Brockwell, J. (1963). Accuracy of a plant infection technique for counting populations of Rhizobium trifolii. Applied Microbiology 11, 377.CrossRefGoogle ScholarPubMed
East of Scotland College of Agriculture (1980). Hill improvement. East of Scotland College of Agriculture, Edinburgh, Bulletin 26.Google Scholar
Frame, J. (1973). Land improvement techniques. Proceedings of Colloquia, No. 3, Potassium Institute, pp. 172–174.Google Scholar
Franklin, M. F. & Mann, A. D. (1980). DSIGNX, Inter University Research Councils Series, Report 48. Edinburgh University, Program Library Unit.Google Scholar
Gibson, A. H. (1978). Recovery and compensation by nodulated legumes to environmental stress. In Plant Relations in Pastures (ed. Wilson, J. R.), pp. 385–403. Australia: C.S.I.R.O.Google Scholar
Haystead, A. & Marriott, C. A. (1978). The fixation and transfer of nitrogen in a clover grass sward under hill conditions. Annals of Applied Biology 88, 453–457.CrossRefGoogle Scholar
Hill Farming Research Organisation (1979). Science and Hill Farming, p. 26. Penicuik: Hill Farming Research Organisation.Google Scholar
Holding, A. J. & King, J. (1963). The effectiveness of indigenous populations of Rhizobium trifolii in relation to soil factors. Plant and Soil 18, 191–198.CrossRefGoogle Scholar
International Biological Programme (1973). World Catalogue of Rhizobium Collections (ed. Skinner, F. A.), pp. 164–165. London: International Biological Programme.Google Scholar
Jones, D. G. (1963). Symbiotic variation in Rhizobium trifolii with S100 Nomark white clover (T. repens L.). Journal of the Science of Food and Agriculture 14, 740–743.CrossRefGoogle Scholar
Jones, D. G. (1966). The contribution of white clover to a mixed upland sward. II. Factors affecting the density and effectiveness of Rhizobium trifolii. Plant and Soil 24, 250–260.CrossRefGoogle Scholar
Jones, D. G., Druce, R. G. & Williams, G. (1967). Comparative trials and seed pelleting, inoculation and the use of high lime dressings in upland reclamation. Journal of Applied Bacteriology 30, 511–517.CrossRefGoogle Scholar
Jones, D. G., Munro, J. M. M., Hughes, R. & Davies, W. E. (1964). The contribution of white clover to a mixed sward. I. The effect of Rhizobium inoculation on the early development of white clover. Plant and Soil 21, 63–69.CrossRefGoogle Scholar
Masterson, C. L. (1973). Recent developments in clover nitrogen fixation. Farm and Food Research 4, 94–96.Google Scholar
Masterson, C. L. & Murphy, P. M. (1976). Application of the acetylene reduction technique to the study of nitrogen fixation by white clover in the field. In Symbiotic Nitrogen Fixation in Plants, IBP 7 (ed. Nutman, P. S.), pp. 299–316. Cambridge University Press.Google Scholar
Masterson, C. L. & Sherwood, M. T. (1974). Selection of Rhizobium trifolii strains by white and subterranean clovers. Irish Journal of Agricultural Research 13, 91–99.Google Scholar
Mytton, L. R. (1975). White clover inoculation and hill land improvement. ARC Research Review 1, 5–8.Google Scholar
Mytton, L. R. & Felice, J. (1977). The effect of mixtures of Rhizobium strains on the dry matter production of white clover grown in agar. Annals of Applied Biology 87, 83–93.CrossRefGoogle Scholar
Newbould, P. (1980). The limitations and potential for pasture production in the hills and uplands. In Forestry and Farming in Upland Britain, pp. 29–75, Forestry Commission Occasional paper 6.Google Scholar
Newbould, P. & Haystead, A. (1978). Trifolium repens(white clover) — its role, establishment and maintenance in hill pastures. Report 7, Hill Farming Research Organisation, 1974–77, 49–68.Google Scholar
North of Scotland College of Agriculture,(1972). The improvement of hill pastures. North of Scotland College of Agriculture, Leaflet 9.Google Scholar
Poulter, A. A. (1933). Deficiency of clover nodule organism. Deficiency of some Welsh soils. The Welsh Journal of Agriculture 9, 145–149.Google Scholar
Reiners, W. A. (1981). Nitrogen cycling in relation to ecosystem succession. In Ecological Bulletin, Stockholm (ed. Clarke, F. E. and Rosswall, T.) 33, 507–528.Google Scholar
Russell, P. E. & Jones, D. G. (1975). Variation in the selection of Rhizobium trifolii by varieties of red and white clover. Soil Biology and Biochemistry 7, 15–18.CrossRefGoogle Scholar
Scottish Agricultural Colleges (1980). Inoculation of white clover seed with rhizobia. The Scottish Agricultural Colleges, Technical Note 35.Google Scholar
Singer, M., Holding, A. J. & King, J. (1964). The response of Trifolium repens to inocula containing varying proportions of effective and ineffective Rhizobium. Proceedings of Sth International Congress SoilSciencelll, 1021–1025.Google Scholar
Sprent, J. I. (1979). The Biology of Nitrogen-fixing Organisms. London: McGraw Hill.Google Scholar
Sprent, J. I. & Bradford, A. M. (1977). Nitrogen fixation in field beans (Vicia faba) as affected by population density, shading and its relationship with soil moisture. Journal of Agricultural Science, Cambridge 88, 303–310.CrossRefGoogle Scholar
Thornton, H. G. (1954). The nodule bacteria and their host legumes. Some problems that they still present. Science Progress 166, 185–204.Google Scholar
Tottingham, W. E. (1914). A quantitative, chemical and physiological study of nutrient solutions for plant culture. (Described solution of Crone, , der, G. Van) Physiological Research 1, 133.Google Scholar
Vallis, I. (1978). Nitrogen relationships in grass/legume mixtures. In Plant Relations in Pastures (ed. Wilson, J. R.), pp. 190–201. Australia: C.S.I.R.O.Google Scholar
Vincent, J. M. (1970). A manual for the practical study of root-nodule bacteria. IBP Handbook 15. Oxford: Blackwell.Google Scholar
Young, D. J. B. (1958). A study of the influence of nitrogen on the root weight and nodulation of white clover in a mixed sward. Journal of the British Grassland Society 13, 106–114.CrossRefGoogle Scholar