Hostname: page-component-84b7d79bbc-5lx2p Total loading time: 0 Render date: 2024-07-30T14:33:16.218Z Has data issue: false hasContentIssue false

Changes in the biochemical composition of herbage upon freezing and thawing

Published online by Cambridge University Press:  27 March 2009

J. C. Macrae
Affiliation:
Hill Farming Research Organization, Bush Estate, Penicuik, Midlothian EH26 OPH
D. R. Campbell
Affiliation:
Hill Farming Research Organization, Bush Estate, Penicuik, Midlothian EH26 OPH
J. Eadie
Affiliation:
Hill Farming Research Organization, Bush Estate, Penicuik, Midlothian EH26 OPH

Summary

Six herbage samples (ranging from poor quality hill herbages to high quality ryegrass and clover) were analysed for soluble carbohydrate, total and soluble nitrogen (N), cell-wall carbohydrates and in vitro digestibility. Changes during freezing and thawing processes were measured. Invertase activity occurred in all frozen and thawed samples. Although the total N content of all samples remained constant throughout, the soluble-N contents of the four better quality herbages were considerably reduced on freezing and thawing. Cell-wall carbohydrates and in vitro digestibility measurements were unaffected by freezing and thawing.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alexander, R. H. & McGowan, M. (1961). A filtration procedure for the in vitro determination of digestibility of herbage. Journal of the British Grassland Society 16, 276–6.CrossRefGoogle Scholar
Bailey, R. W. (1958). The reaction of pentoses with anthrone. Biochemical Journal 68, 669–72.Google Scholar
Bailey, R. W. (1964). Pasture quality and ruminant nutrition. I. Carbohydrate composition of ryegrass varieties grown as sheep pastures. New Zealand Journal of Agricultural Research 7, 496507.CrossRefGoogle Scholar
Beever, D. E., Cammell, S. B. & Wallace, A. S. (1974). The digestion of fresh, frozen and dried perennial ryegrass. Proceedings of the Nutrition Society 33, 73A.Google Scholar
Bryant, A. M. (1971). In Research in the New Zealand Department of Agriculture, Annual Report of Research Divisions (1970–71), p. 50.Google Scholar
Crook, E. M. (1946). The extraction of nitrogenous materials from green leaves. Biochemical Journal 40, 197209.CrossRefGoogle ScholarPubMed
Ekern, A., Blaxter, K. B. & Sawers, D. (1965). The effect of artificial drying and of freezing on the energy value of pasture herbage. In Energy Metabolism (ed. Blaxter, K. L.), pp. 217–24. London: Academic Press.Google Scholar
Gutteridge, J. M. C. & Wright, E. B. (1968). A simple automated guaiacum glucose-oxidase method. Journal of Medical Laboratory Technology 25, 385–6.Google ScholarPubMed
King, J. & Nicholson, I. A. (1964). Grasslands of the forest and sub-alpine zones. In The Vegetation of Scotland (ed. Burnett, J. H.), ch. 6. Edinburgh: Oliver & Boyd.Google Scholar
Lyttleton, J. W. (1956). Protein of pasture plants. Cytoplasmic protein of White Clover and Italian Ryegrass. Biochemical Journal 64, 7080.CrossRefGoogle ScholarPubMed
MacRae, J. C. (1970). Changes in chemical composition of freeze-stored herbage. New Zealand Journal of Agricultural Research 13, 4550.CrossRefGoogle Scholar
MacRae, J. C. & Ulyatt, M. J. (1974). Quantitative digestion of fresh herbage by sheep. II. The sites of digestion of some nitrogenous constituents. Journal of Agricultural Science, Cambridge 82, 309–19.CrossRefGoogle Scholar
Raymond, W. F., Harris, C. E. & Harkbr, V. G. (1953). Studies on the digestibility of herbage. II. Effect of freezing and cold storage of herbage on its digestibility by sheep. Journal of the British Grassland Society 8, 315–20.CrossRefGoogle Scholar
Smith, D. & Grotelueschen, R. D. (1966). Carbohydrates in grasses. I. Sugar and fructosan composition of the stem bases of several northern-adapted grasses at seed maturity. Crop Science 6, 263–6.CrossRefGoogle Scholar
Somogyi, M. (1945). Determination of blood sugar. Journal of Biological Chemistry 160, 6973.CrossRefGoogle Scholar
Van Soest, P. J. (1965). Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fibre and lignin. Journal of Association of Official Agricultural Chemists 46, 829–35.Google Scholar
Van Soest, P. J. & Wine, R. H. (1967). Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents. Journal of the Association of Official Agricultural Chemists 50, 50–5.Google Scholar