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Use of the entomopathogenic fungi Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea to control Diaphorina citri (Hemiptera: Psyllidae) in Persian lime under field conditions

Published online by Cambridge University Press:  12 March 2012

Roberto Lezama-Gutiérrez ,
Jaime Molina-Ochoa ,
Omar Chávez-Flores ,
César Andrés Ángel-Sahagún ,
Steven R. Skoda ,
Gerardo Reyes-Martínez ,
Marisela Barba-Reynoso ,
Oscar Rebolledo-Domínguez ,
Graciela M.L. Ruíz-Aguilar  and
John E. Foster
Roberto Lezama-Gutiérrez
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Apartado Postal 36, Km. 40 autopista Colima-Manzanillo, Tecomán, Colima28930, Mexico
Jaime Molina-Ochoa*
Affiliation:
División Ciencias de la Salud e Ingenierías, Departamento Agroindustrial, Universidad de Guanajuato, Campus Celaya-Salvatierra, Salvatierra, Guanajuato38900, Mexico Department of Entomology, Insect Genetics Laboratory, University of Nebraska-Lincoln, 3B Entomology Hall, Lincoln, NE68583-0816, USA
Omar Chávez-Flores
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Apartado Postal 36, Km. 40 autopista Colima-Manzanillo, Tecomán, Colima28930, Mexico
César Andrés Ángel-Sahagún
Affiliation:
División de Ciencias de la Vida, Departamento de Agronomía, Campus Irapuato-Salamanca, Universidad de Guanajuato, exhacienda El Copal, Km. 7, Carretera Irapuato-Silao, Apartado Postal 311, Irapuato, Guanajuato, CP 36500, Mexico
Steven R. Skoda
Affiliation:
USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX78028, USA
Gerardo Reyes-Martínez
Affiliation:
Consejo Estatal de Productores de Limón (COEPLIM), Abasolo 475, Colonia Centro, Tecomán, Colima, CP 28100, Mexico
Marisela Barba-Reynoso
Affiliation:
Consejo Estatal de Productores de Limón (COEPLIM), Abasolo 475, Colonia Centro, Tecomán, Colima, CP 28100, Mexico
Oscar Rebolledo-Domínguez
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Apartado Postal 36, Km. 40 autopista Colima-Manzanillo, Tecomán, Colima28930, Mexico
Graciela M.L. Ruíz-Aguilar
Affiliation:
División Ciencias de la Salud e Ingenierías, Departamento Agroindustrial, Universidad de Guanajuato, Campus Celaya-Salvatierra, Salvatierra, Guanajuato38900, Mexico
John E. Foster
Affiliation:
Department of Entomology, Insect Genetics Laboratory, University of Nebraska-Lincoln, 3B Entomology Hall, Lincoln, NE68583-0816, USA
*
*E-mail: jmolina18@hotmail.com; jmolinao@ugto.mx
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Abstract

The Asian citrus psyllid Diaphorina citri Kuwayama is a destructive insect pest in citriculture, because it is an efficient vector of the proteobacteria ‘Candidatus Liberibacter asiaticus’ (Las), ‘Ca. L. africanus’ (Laf) and ‘Ca. L. americanus’ (Lam). These bacteria cause the ‘huanglongbing’ disease or ‘greening’ or ‘yellow dragon’ disease. The disease kills the plant and reduces fruit production. This insect pest is susceptible to entomopathogenic fungi, and we report the use of different strains of Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea against the nymphs and adults of D. citri under field conditions. The fungi were applied four times using a concentration of 2 × 1013 conidia/ha with a time interval of 15 days between applications. The percentage of control of Cb 108, Ma 65, Ma 14 and Ifr 4 was 60, 50, 40 and 35% in nymphs, and 50, 50, 42 and 22% in adults, respectively. Metarhizium anisopliae, C. bassiana and I. fumosorosea applied on Persian lime groves are more effective in reducing higher density of nymphs than adults of D. citri.

Keywords

Diaphorina citrihuanglongbingPersian limeMexican limebiological control
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 32 , Issue 1 , March 2012 , pp. 39 - 44
Copyright
Copyright © ICIPE 2012

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