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The Limit of Tolerance to Heat Pulses During Embryogenesis in Ceratitis capitata Wiedemann (Diptera; Tephritidae)

Published online by Cambridge University Press:  19 September 2011

Jorge L. Cladera
Affiliation:
Departamento de Genètica, INTA, C.C. 25, 1712 Castelar, Argentina
Fanny C. Manso
Affiliation:
Departamento de Genètica, INTA, C.C. 25, 1712 Castelar, Argentina
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Abstract

The limit of tolerance to temperature treatments that a developing embryo of Ceratitis capitata is able to withstand without suffering a reduction in hatchability or an increase in late abortions was investigated. Major events during embryogenesis were timed and the observation of the chitinous mouth hook inside unhatched eggs was chosen as a marker of late embryo death. This late abortion production was investigated in three different embryo stages and using two pulse lengths. It was found that an exposure of embryos aged 16 hr after egg laying (right before the beginning of the head involution) to 35°C for a period of 15 hr is close enough but below the limit of tolerance for the strain T (Y-nig+)5038 used in this investigation. Consequently, this exposure is proposed as the discriminating treatment in a screening protocol designed for the isolation of a temperature-sensitive embryonic-lethal mutation.

Résumé

Nous présentons ici, les résultats de notre travail pour connaitre la limite de tolerance des traitements thermiques chez l'embryon de Ceratitis capitata, sans produire ni la réduction des naissances ni l'augmentation d'avortements tardifs. Pendant l'embryogenèse quelques événements importants ont été chronométrés, en choisissant d'entre eux l'observation du crochet bucai, dedans l'oeuf, comme sympthome de mort embryonnaire tardive. Les avortements chez l'embryon ont été observés à trois stades et deux expositions de different durée. On a trouvé que les embryons, âgés 16 hs, exposés pendant 15hs à 35°C, s'approchaient au limite de tolerance pour la ligne T (Y-nig+)5038, utilisée a cette recherche. Par conséquent, on propose ce traitement discriminant qui a été dessiné afin d'isoler une mutation létal embryonnaire susceptible à la temperature élevée.

Type
Research Articles
Copyright
Copyright © ICIPE 1988

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References

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