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Influence of indoor microclimate and diet on survival of Anopheles gambiae s.s. (Diptera: Culicidae) in village house conditions in western Kenya

  • Bernard A. Okech (a1) (a2) (a3), Louis C. Gouagna (a1), Bart G.J. Knols (a1), Ephantus W. Kabiru (a1), Gerry F. Killeen (a1), John C. Beier (a4), Guiyun Yan (a5) and John I. Githure (a1)...


The survival of female Anopheles gambiae s.s. mosquitoes inside two village house types (grass-thatched and iron-roofed) was studied in relation to diet and ambient indoor microclimatic conditions. Two batches of 20–30, 1-day-old laboratory-bred mosquitoes were maintained inside cages in the grass-thatched (n=2) and iron-roofed (n=2) houses and fed daily, one group on 10% glucose and the other on human blood. Throughout the experiments, indoor temperature and relative humidity of the houses were recorded, and mortality of mosquitoes monitored daily until all had died. The experiments were replicated thrice. There was no significant variation in the overall mean temperature (P=0.93) or relative humidity profiles (P=0.099) between the two house types, although the iron-roofed houses recorded higher temperature peaks. A Kaplan–Meier survival analysis showed that the mean survival times of mosquitoes were 8 and 10 days in the two grass-thatched huts and 7 and 10 days in the two iron-roof houses for mosquitoes feeding on blood and sugar meals, respectively. The mean survival times of mosquitoes maintained inside similar house types differed only due to diet. In the proportionality of hazards model (Cox regression), the dietary regimes significantly influenced the probability of survival (P=0.0001), with mosquitoes surviving longer on sugar meals than on blood. Microclimatic factors inside houses also significantly influenced mosquito survival. Although higher peak temperatures were recorded in corrugated iron-roofed houses, the survival of the mosquitoes resting in them did not differ significantly from that in grass-thatched houses. However, the impact of these temperatures on the development of malaria parasites inside the vector needs to be investigated.


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Beier, J. C. (1996) Frequent blood-feeding and restrictive sugar-feeding behavior enhance the malaria vector potential of Anopheles gambiae s.l. and An. funestus (Diptera: Culicidae) in western Kenya. J. Med. Entomol. 33, 613618.
Benedict, M. Q. (1997) In Molecular Biology of Insect Disease Vectors: A Methods Manual (Edited by Crampton, J. M., Beard, C. B. and Louis, C.) Chapman and Hall.
Briegel, H., Horler, E. (1993) Multiple blood meals as a reproductive strategy in Anopheles (Diptera: Culicidae). J. Med. Entomol. 30, 975985.
Clements, A. N. (1963) The Physiology of Mosquitoes. Macmillan, New York.
Coluzzi, M. (1984) Heterogeneities of malaria vectorial system in the tropical Africa and their significance in malaria epidemiology and control. Bull. W.H.O. 62, 107113.
Copeland, R. S. (1994) Anopheles mosquitoes: parasite vector interactions, host vector interactions and population management. In Proceedings of the 3rd International Conference on Tropical Entomology (Edited by Saini, R. K.). Nairobi, Kenya.
Craig, M. H., Snow, R. W., le Sueur, D. (1999) A climate-based distribution model of malaria transmission in sub-Saharan Africa. Parasitol. Today 15, 105111.
Gamage-Mendis, A. C., Carter, R., Mendis, C. De, Zoysa, A. P., Herath, P. R., Mendis, K. N. (1991) Clustering of malaria infections within an endemic population: risk of malaria associated with the type of housing construction. Am. J. Trop. Med. Hyg. 45, 7785.
Githeko, A. K., Service, M. W., Mbogo, C. M., Atieli, F. K., Juma, F. O. (1994) Origin of blood meals in indoor and outdoor resting malaria vectors in western Kenya. Acta Trop. 58, 307316.
Lindsay, S. W., Snow, R. W. (1988) The trouble with eaves; house entry by vectors of malaria. Trans. R. Soc. Trop. Med. Hyg. 82, 645646.
Minakawa, N., Githure, J. I., Beier, J. C., Yan, G. (2001) Anopheline mosquito survival strategies during the dry period in western Kenya. J. Med. Entomol. 38, 388392.
Minakawa, N., Mutero, C. M., Githure, J. I., Beier, J. C., Yan, G. (1999) Spatial distribution and habitat characterization of anopheline mosquito larvae in Western Kenya. Am. J. Trop. Med. Hyg. 61, 10101016.
Minakawa, N., Seda, P., Yan, G. (2002) Influence of host and larval habitat distribution on the abundance of African malaria vectors in western Kenya. Am. J. Trop. Med. Hyg. 67, 3238.
Mutero, C. M., Ouma, J. H., Agak, B. K., Wanderi, J. A., Copeland, R. S. (1998) Malaria prevalence and use of self-protection measures against mosquitoes in Suba District, Kenya. East Afr. Med. J. 75, 1115.
Okech, B. A., Gouagna, L. C., Killeen, G. F., Knols, B. G., Kabiru, E. W., Beier, J. C., Yan, G., Githure, J. I. (2003) Influence of sugar availability and indoor microclimate on survival of Anopheles gambiae (Diptera: Culicidae) under semifield conditions in western Kenya. J. Med. Entomol. 40, 657663.
Omer, S. M., Cloudsley-Thompson, J. L. (1970) Survival of female Anopheles gambiae Giles through nine month dry season in Sudan. Bull. WHO 42, 319330.
Ribeiro, J. M. C., Seulu, F., Abose, T., Kidane, G., Teklehaimanot, A. (1996) Temporal and spatial distribution of anopheline mosquitoes in an Ethiopian village: implications for malaria control strategies. Bull. WHO 74, 299305.
Service, M. W. (1973) Identification of predators of Anopheles gambiae resting in huts, by the precipitin test. Trans. R. Soc. Trop. Med. Hyg. 67, 3344.
Shililu, J., Mbogo, C., Mutero, C., Gunter, J., Swalm, C., Regens, J., Keating, J., Yan, G., Githure, J., Beier, J. (2003) Spatial distribution of Anopheles gambiae and Anopheles funestus and malaria transmission in Suba District, western Kenya. Insect Sci. Applic. 23, 187196.
Smith, T., Charlwood, J. D., Takken, W., Tanner, M., Spiegelhalter, D. J. (1995) Mapping densities of malaria vectors within a single village. Acta Trop. 59, 118.
White, G. B. (1974) Anopheles gambiae complex and disease transmission in Africa. Trans. R. Soc. Trop. Med. Hyg. 68, 278301.
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International Journal of Tropical Insect Science
  • ISSN: 1742-7584
  • EISSN: 1742-7592
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