Hostname: page-component-77c89778f8-n9wrp Total loading time: 0 Render date: 2024-07-20T22:56:22.878Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of protein fractions isolated from cereal grains on the development and survival of the cowpea weevil, Callosobruchus maculatus

Published online by Cambridge University Press:  19 September 2011

L. B. S. Andrade ,
V. M. Q. Flores ,
M. P. Sales ,
R. A. Morais ,
A. Blanco-Labra  and
J. Xavier-Filho
L. B. S. Andrade
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
V. M. Q. Flores
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
M. P. Sales
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
R. A. Morais
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
A. Blanco-Labra
Affiliation:
Centro de Investigacion y de Estudios Avanzados del I.P.N—Unidad Irapuato, Carretera Irapuato-Leon, Irapuato, Gto, Mexico
J. Xavier-Filho
Affiliation:
Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970 Fortaleza, Ceará, Brazil
Get access

Abstract

The effect of protein fractions isolated from cereal grains on the development and survival of the bean weevil, Callosobruchus maculatus, was investigated using wheat, rice and maize. These cereals were fractionated into albumins, globulins, prolamins, glutelins and a residue. The fractions were incorporated in artificial seeds which were infested by C. maculatus. Survival (as indicated by LD50) and development (as indicated by WD50) of 20-day-larvae of the bruchid were negatively affected by most of the fractions. Rice globulins were the most detrimental and affected both the survival and development of the larvae, while wheat albumins and globulins had a stronger effect on larval development than on survival. A previously isolated maize α-amylase/trypsin inhibitor showed an LD50 of 1.5% to Callosobruchus maculatus.

Résumé

L'effet des fractions protéiniques des semences de blé de riz et de maïs a été étudié sur le développement et la survie du bruchide du niébé, Callosobruchus maculatus. Les céréales ont été fractionnées en albumines, globulines, prolamines, glutélines et en un résidu, avec les fractions incorporées dans des semences artificielles infestées du bruchide, Callosobruchus maculatus. La survie (telle qu'indiquée par la CL50) et le développement (tel qu'indiqué par WD50) des larves a âgées de 20 jours ont été négativement affectés par la plupart des fractions. Les globulines du riz ont été les plus nocives, affectant à la fois la survie et le développement des larves, tandis que l'effet des albumines et de globulines du blé a été plus marqué sur le développement larvaire que sur la survie. Un inhibiteur de la α-amylase/trypsine auparavant isolé du maïs, a révélé une CL50 de 1,5% sur Callosobruchus maculatus.

Keywords

cowpea weevilCallosobruchus maculatuswheatricemaizecereal grainsprotein fractionsinsecticidal proteinsbruchide du niébéCallosobruchus maculatusblérizmaïssemences de céréalesfractions protéiniquesprotéines à action insecticide
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 16 , Issue 2 , June 1995 , pp. 171 - 176
Copyright
Copyright © ICIPE 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Blanco-Labra, A. and Iturbe-Chinas, F. A. (1981) Purification and characterization of an α-amylase inhibitor from maize seeds (Zea mays). J. Food Biochem. 5, 1–17.CrossRefGoogle Scholar
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.CrossRefGoogle ScholarPubMed
Bright, S. W. S. and Shewry, P. R. (1983) Improvement of protein quality in cereals. CRC Critical Rev. Plant Science 1, 49–93.CrossRefGoogle Scholar
Bunce, N. A. C., White, R. P. and Shewry, P. R. (1985) Variation in estimates of molecular weights of cereal prolamins by SDS-PAGE. J. Cereal Chem. 3, 131–142.CrossRefGoogle Scholar
Daoust, R. A., Roberts, D. A. and Neves, B. P. (1985) Distribution, biology and control of cowpea pests in Latin America. In Cowpea Research, Production and Utilization (Edited by Singh, S. R. and Rachie, K. O.), pp. 249–264. John Wiley, London.Google Scholar
Das, G. P. (1986) Effect of different concentrations of neem oil on the adult mortality and oviposition of Callosobruchus chinensis Linn. (Bruchidae: Coleoptera). Indian J. Agric. Sci. 56, 743–744.Google Scholar
Firmino, F., Fernandes, K. V. S., Sales, M. P., Gomes, V. M., Alcantara, M. R. M., Domingues, S. J. S. and Xavier-Filho, J. (1996) Cowpea (Vigna unguiculata) vicilins associate with putative chitinous structures in midgut and feces of the bruchid beetles Callosobruchus maculatus and Zabrotes subfasciatus. Braz. J. Med. Biol. Res. 29, 749–756.Google Scholar
Hilder, V. A., Gatehouse, A. M. R., Sheerman, S. E., Barker, R. F. and Boulter, D. (1987) A novel mechanism of insect resistance engineered into tobacco. Nature 330, 160–163.CrossRefGoogle Scholar
Jackai, L. E. N. and Daoust, R. A. (1986) Insect pests of cowpeas. Annu. Rev. Entomol. 31, 95–119.CrossRefGoogle Scholar
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Latunde-Dada, A. O. (1990) Genetic manipulation of the cowpea (Vigna unguiculata [L.] Walp.) for enhanced resistance to fungal pathogens and insect pests. Adv. Agronomy 44, 133–154.CrossRefGoogle Scholar
Liang, C., Brookhart, G., Feng, G. H., Reeck, G. R. and Kramer, K. J. (1991) Inhibition of digestive proteinases of stored grain Coleoptera by oryzacystatin, a cysteine proteinase inhibitor from rice seed. FEBS Letters 278, 139–142.CrossRefGoogle ScholarPubMed
Macedo, M. L. R., Andrade, L. B. S., Moraes, R. A. and Xavier-Filho, J. (1993) Vicilin variants and the resistance of cowpea (Vigna unguiculata) seeds to the cowpea weevil (Callosobruchus maculatus). Comp. Biochem. Physiol., 105C, 89–94.Google Scholar
MacGaughey, W. H. and Whalon, M. E. (1992) Managing insect resistance to Bacillus thuringiensis toxins. Science 258, 1451–1455.CrossRefGoogle Scholar
Marques, M. R., Albuquerque, L. M. B. and Xavier-Filho, J. (1992) Antimicrobial and insecticidal activities of cashew tree gum exudate. Ann. Appl. Biol. 121, 371–377.CrossRefGoogle Scholar
Matos, D. G. G., Lima, C. S., Macedo, M. L. R. and Xavier-Filho, J. (1993) Effects of papain and trypsin inhibitors of seeds of Vigna unguiculata on development and survival of the cowpea weevil (Callosobruchus maculatus). XXII Meeting of the Brazilian Biochemical and Molecular Biology Society SBBq, Caxambu, MG, Brazil. Abstract Book pg 45.Google Scholar
Murdock, L. L., Huesing, J. E., Nielsen, S. S., Pratt, R. C. and Shade, R. E. (1990) Biological effects of plant lectins on the cowpea weevil. Phytochemistry 29, 85–89.CrossRefGoogle Scholar
Sales, M. P., Gomes, V. M., Fernandes, K. V. S. and Xavier-Filho, J. (1996) Chitin binding proteins from cowpea and other legume seeds. Braz. J. Med. Biol. Res. 29, 319–326.Google Scholar
Schmutterer, H. (1990) Properties and potential of natural pesticides from the neem tree, Azadirachta indica. Annu. Rev. Entomol. 35, 271–297.CrossRefGoogle ScholarPubMed
Silva, C. P. and Xavier-Filho, J. (1991) Comparison between the levels of aspartic and cysteine proteinases of the larval midguts of Callosobruchus maculatus (F.) and Zabrotes subfasciatus (Boh.) (Coleoptera: Bruchidae). Comp. Biochem. Physiol. 99B, 529–533.Google Scholar
Singh, B. B., Singh, S. R. and Adjadi, O. (1985) Bruchid resistance in cowpea. Crop Science 25, 736–739.CrossRefGoogle Scholar
Singh, S. R., Luse, R. A., Leuschner, K. and Nangju, D. (1979) Groundnut oil for control of cowpea weevil. J. Stored Prod. Res. 14, 77–80.CrossRefGoogle Scholar
Xavier-Filho, J., Campos, F. A. P., Ary, M. B., Silva, C. P., Carvalho, M. M. M., Macedo, M. L. R., Lemos, F. J. A. and Grant, G. (1989) Poor correlation between the levels of proteinase inhibitors found in seeds of different cultivars of cowpea (Vigna unguiculata) and the resistance/susceptibility to predation by Callosobruchus maculatus. J. Agric. Food Chem. 37, 1139–1143.CrossRefGoogle Scholar