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Characterization of antigens from extracts of fed ticks using sera from rabbits immunized with extracted tick antigen and by successive tick infestation

Published online by Cambridge University Press:  19 September 2011

A. O. Mongi ,
S. Z. Shapiro ,
J. J. Doyle  and
M. P. Cunningham
A. O. Mongi
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
S. Z. Shapiro
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
J. J. Doyle
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
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Abstract

Immune resistance to infestation by an ixodid tick, Rhipicephalus appendiculatus, the vector of the African cattle disease, East Coast Fever, was induced in rabbits by either repeated tick feeding or immunization with tick extracts. In addition to resistance to tick infestation, tick extract immunization led to a reduction in the viability of eggs laid by ticks feeding on the immunized host. Resistance to infestation by ixodid ticks has previously been reported by others to have a humoral immune component. Therefore, antibodies from resistant host animals were used to detect the tick antigens they recognized as an approach to identification of the target antigen(s) for the observed immune responses on feeding ticks. In crossed immune electrophoresis two antigens were detected using sera from animals made resistant by multiple tick infestations. Sera from extract immunized animals detected these antigens and nine others. The tick antigens detected by both sets of sera in crossed immune electrophoresis were radiolabelled with [35S]amino acids. No labelled antigens were detected by Staphylococcus aureus mediated immune precipitation with sera from hosts made resistant by multiple infestations. Antibodies from extract-immunized animals identified nine protein antigens by S. aureus immunoprecipitation. The molecular weights of these antigens as assessed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis were 180,000; 140,000; 130,000; 98,000; 92,000; 88,000; 85,000 and 82,000. The rate of synthesis of these antigens appeared to vary in relation to the tick feeding cycle.

Résumé

La resistance immunitaire à l'infestation par les tiques Rhipicephalus appendiculatus vecteur de la fièvre costale est-africaine chez le bétail, est induite chez le lapin aussi bien par piqûre répétée des tiques sur l'animal que par immunisation avec des extraits des tiques. En plus de la resistance à l'infestation par les tiques, l'extrait d'immunisation provoque une reduction de la viabilité des oeufs produits par des tiques nourries sur des animaux immunisés. La resistance à l'infestation par des tiques (ixodides) a déjà été rapporté par d'autres comme etant un composé humoral immunitaire. Aussi les anticorps provenant des animaux resistants furent utilisés pour détecter les antigènes des tiques qui'ls reconnaissent comme approche pour l'identification des antigènes cibles pour l'observation des réponses immunitaires sur des tiques s'alimentant. Par immunoélectrophorèse croisée on a détecté des antigènes en utilisant des sera provenant des animaux rendus résistants par des multiples infestations par des tiques.

Les sera provenant des extraits d'animaux immunisés détectent ces antigènes en plus de neuf autres. Les antigenes des tiques détectés par les deux lots de sera en immunoélectrophorèse croisée ont été marqués avec les [35S]acides aminés. Aucun marquage d'antigènes n'a été détecté par Staphylococcus aureus dirigeant une précipitation immunologigue avec les sera provenant des animaux hotes rendus resistants par des multiples infestations. Les anticorps provenant des animaux immunisés par des extraits des tiques parviennent à identifier neuf antigènes par immunoprecipitation avec S. aureus Les poids moléculaires de ces antigènes révélés par electrophorèse en SDS en gel de plyacrylamide sont respectivement de 180.000; 140.000; 130.000; 98.000; 92.000; 88.000; 85.000 and 82.000. Le taux de synthèse de ces antigènes semblent varier en relation avec le cycle de piqûre des tiques.

Keywords

Rhipicephalus appendiculatusradiolabelled antigensimmunizationimmunodiffusioncrossed immunoelectrophoresisimmunoprecipitationStaphylococcus aureusRhipicephalus appendiculatusantigènes marquésimmunisationimmunoddifusionimmunoélectrophorèse croiséeimmunoprécipitationStaphylococcus aureus
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 7 , Issue 4 , August 1986 , pp. 479 - 487
Copyright
Copyright © ICIPE 1986

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