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Biochemical evidence for the feeding sites of the leafhopper Nephotettix virescens Within susceptible and resistant rice plants

Published online by Cambridge University Press:  19 September 2011

J. L. Auclair ,
E. Baldos  and
E. A. Heinrichs
J. L. Auclair*
Affiliation:
International Rice Research Institute, P.O. Box 933, Manila, the Philippines
E. Baldos
Affiliation:
International Rice Research Institute, P.O. Box 933, Manila, the Philippines
E. A. Heinrichs
Affiliation:
International Rice Research Institute, P.O. Box 933, Manila, the Philippines
*
*To whom correspondence should be addressed at: Département de Sciences Biologiques, Université de Montréal, Suce. A. C.P. 6128. Montréal, P.Q. Canada H3C 3J7.
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Abstract

Semi-quantitative biochemical analyses by paper chromatography–densitometry and pH determinations of honeydew excreted by Nephotettix virescens (Distant) feeding on rice plants revealed higher free amino compound and sugar concentrations in honeydew excreted on the susceptible variety TN1 than on resistant ASD7 or P203. Furthermore, sap exudates from these three varieties, presumed to be mainly from xylem, as well as honeydew excreted on the resistant varieties were acidic, whereas honeydew from TN1 was predominantly basic. Since phloem sap in herbaceous plants usually gives an alkaline reaction whereas xylem sap is acidic, and because the former contains much higher concentrations of sugars and free amino acids than the latter, the biochemical evidence strongly suggests that Nephotettix virescens is primarily a xylem feeder on the resistant plants tested, but feeds predominantly on phloem sap in susceptible TNI.

Résumé

Des analyses semi-quantitatives par chromatographie sur papier et densitométrie et des déterminations du pH ont été effectuées sur le miellat excrété par Nephotettix virescens (Distant) se nourrissant sur des plantes de riz. Les résultats ont indiqué une teneur beaucoup plus élevée en acides aminés libres et en sucre dans le miellat excrété sur la variété sensible de riz TN1 que sur les variétés résistantes ASD7 ou P203. De plus, des exsudats de sève brute provenant des vaisseaux du bois (xylème) de ces trois variétés, de même que le miellat excrété sur les variétés résistantes étaient de réaction acide, tandis que le miellat excrété sur la variété sensible était généralement alcalin. Puisque la sève élaborée du phloème chez les plantes herbacées est généralement de réaction alcaline, tandis que celle provenant des vaisseaux du bois est acide, et que la sève élaborée contient généralement une teneur beaucoup plus élevée en acides aminés et en sucres, les résultats d'analyse biochimique suggèrent fortement que Nephotettix virescens se nourrit principalement de la sève brute sur les deux variétés résistantes, mais surtout de sève élaborée chez la variété sensible TN1.

Keywords

Nephotettix virescensfeeding sitesrice plantsaphoneydewNephotettix virescenssites de prise de nourritureriz
Type
Articles
Information
International Journal of Tropical Insect Science , Volume 3 , Issue 1 , March 1982 , pp. 29 - 34
Copyright
Copyright © ICIPE 1982

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