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Bioassay of insecticidal Bacillus thuringiensis Cry IA delta-endotoxins against the spotted bollworm, Earias vittella Fabricius

Published online by Cambridge University Press:  19 September 2011

Gagana Kumar Mahapatro ,
Gorakh Prasad Gupta  and
P. Ananda Kumar
Gagana Kumar Mahapatro
Affiliation:
Division of Entomology
Gorakh Prasad Gupta
Affiliation:
Division of Entomology
P. Ananda Kumar
Affiliation:
National Research Centre (NRC) on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India
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Abstract

Insecticidal δ-endotoxins of Bacillus thuringiensis, Cry IA (a, b and c) were prepared from over-expressing cells of Escherichia coli and bioassayed against the third instars (6-day-old) of the spotted bollworm, Earias vittella Fabricius (Lepidoptera: Noctuidae), using artificial diet. The median lethal concentration (LC50) values were calculated for 6 days of exposure to δ-endotoxins. Cry IA(a) was found to be the most toxic (LC50 141.2 ng/cm2) followed by Cry IA(a) and Cry IA(b) with LC50 values of 224.5 and 300.5 ng/cm2 respectively. A highly significant reduction in larval weight took place in the surviving larvae treated with sub-lethal doses of the insecticidal crystal proteins.

Résumé

Les delta-endotoxines insecticides de Bacillus thuringiensis, Cry IA (a, b et c) ont été obtenues à partir de cellules d'Escherichia coli ayant un taux d'expression élevé et testées sur des chenilles du 3ème stade (âgées de 6 jours) du ver de la capsule Earias vittella Fabricius (Lepidoptera: Noctuidae), élevées sur milieu artificiel. Les valeurs de la dose létale moyenne (DL50) ont été calculées pour 6 jours d'exposition aux delta-endotoxines. Cry IA(c) est la plus toxique (DL50, 141,2 ng/cm2), suivie de Cry IA(a) et Cry IA(b) avec une DL50 de 224,5 et 300,5 ng/cm2 respectivement. Une réduction significative de poids a été constatée chez les chenilles survivantes traitées avec des doses sublétales des protéines cristallines insecticides.

Keywords

Bacillus thuringiensisinsecticidal crystal proteinsCry IAδ-endotoxinsspotted bollwormEarias vittellaBacillus thuringiensisprotéines cristallines insecticidesCry IAδ-endotoxinesver de la capsuleEarias vittella
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 21 , Issue 3 , September 2001 , pp. 225 - 228
Copyright
Copyright © ICIPE 2001

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