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Molecular Nosocomial Epidemiology: High Speed Typing of Microbial Pathogens by Arbitrary Primed Polymerase Chain Reaction Assays

Published online by Cambridge University Press:  02 January 2015

Alex van Belkum ,
Willem van Leeuwen ,
Jan Kluytmans  and
Henri Verbrugh
Alex van Belkum*
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
Willem van Leeuwen
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
Jan Kluytmans
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
Henri Verbrugh
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
*
Dr. Molewaterplein 40, Department of Bacteriology, University Hospital Rotterdam, 3015 GD Rotterdam, The Netherlands
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Abstract

Arbitrary primed polymerase chain reaction (AP PCR) assays are suited for the discrimination of isolates of all clinically relevant bacterial species. In a hospital setting, this type of DNA amplification test can be used for the timely detection of ongoing nosocomial outbreaks. For rapid screening of isolates of many medically important bacterial species, including Acinetobacter baumannii, Staphylococcus aureus, Serratia marcescens, Xanthomonas maltophilia and others, a single AP PCR assay can be used as a primary typing screen for genetic relatedness. In combination with epidemiological data, AP PCR testing is particularly useful for identifying true outbreaks caused by a single strain.

Type
Molecular Hospital Epidemiology
Information
Infection Control & Hospital Epidemiology , Volume 16 , Issue 11 , November 1995 , pp. 658 - 666
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1995

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References

1.Maslow, JN, Mulligan, ME, Arbeit, RD. Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin Infect Dis 1993;17:153–164.Google Scholar
2.Jarvis, WR. Usefulness of molecular epidemiology for outbreak investigations. Infect Control Hosp Epidemiol 1994;15:500–503.Google Scholar
3.Bingen, E. Applications of molecular methods to epidemiologic investigations of nosocomial infections in a pediatric hospital. Infect Control Hosp Epidemiol 1994;15:488–493.Google Scholar
4.van Belkum, A. DNA fingerprinting of medically important microorganisms by use of PCR. Clin Microbiol Rev 1994;7:174–184.Google Scholar
5.Jeffreys, AJ, Macleod, A, Tamaki, K, Neil, DC, Monckton, DG. Minisatellite repeat coding as a digital approach to DNA typing. Nature (London) 1991;354:204–209.Google Scholar
6.Hayes, LJ, Bailey, RL, Mabey, DC, et al.Genotyping of Chlamydia trachomatis in a trachoma endemic village in the Gambia by a nested polymerase chain reaction: identification of strain variants. J Infect Dis 1992;166:1173–1177.Google Scholar
7.Tompkins, LS. The use of molecular methods in infectious diseases. N Engl J Med 1992;327:1290–1297.Google Scholar
8.Woods, CR, Versalovic, J, Koeuth, T, Lupski, JR. Whole-cell repetitive element sequence based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. J Clin Microbiol 1993;31:1927–1931.Google Scholar
9.Boom, R, Sol, CJA, Salimans, MMM, Jansen, CL, Wertheim van Dillen, PME, van der Noordaa, J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990;28:495–503.Google Scholar
10.Versalovic, J, Koeuth, T, Lupski, JR. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991;19:6823–6831.Google Scholar
11.Blumberg, HM, Rimland, D, Kiehlbauch, JA, Terry, PM, Wachsmuth, IK. Epidemiologic typing of Staphylococcus aureus by DNA restriction fragment-length polymorphisms of rRNA genes: elucidation of the clonal nature of a group of bacteriophage nontypeable, ciprofloxacin-resistant, methicillin-susceptible S aureus isolates. J Clin Microbiol 1992;30:362–369.Google Scholar
12.van Belkum, A, Bax, R, Peerbooms, P, Goessens, WHF, van Leeuwen, N, Quint, WGV. Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 1993;31:798–803.Google Scholar
13.van Leeuwen, WJ, Rost, JA. Additional set of phages of S aureus strains nontypeable with the international basic set of phages. Zentralblatt Fur Bakteriologie 1976;H49(suppl 5):1013–1019. Abstract I.Google Scholar
14.Kropp, H, Gerckens, L, Sundelof, JG, Kahan, FM. Antibacterial activity of imipenem: the first thienamycin antibiotic. Rev Infect Dis 1985;7:S389–S410.Google Scholar
15.Struelens, MJ, Carlier, E, Maes, N, Serruys, E, Quint, WGV, van Belkum, A. Nosocomial colonization and infection with multi-resistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR fingerprinting. J Hosp Infect 1993;25:15–32.Google Scholar
16.Chetchotsisakd, P, Phelps, CL, Hartstein, AI. Assessment of bacterial cross-transmission as a cause of infections in patients in intensive care units. J Infect Dis 1994;18:929–937.Google Scholar
17.Veringa, E, van Belkum, A, Schellekens, H. Iatrogenic meningitis by Streptococcus salivarius following lumbar punction. J Hosp Infect 1995;44:316–318.Google Scholar
18.Tompkins, LS, Tenover, F, Arvin, A. New technology in the clinical microbiology laboratory: what you always wanted to know but were afraid to ask. J Infect Dis 1994;170:1068–1074.Google Scholar
19.Goering, RV. Molecular epidemiology of nosocomial infection: analysis of chromosomal restriction fragment patterns by pulsed-field gel electrophoresis. Infect Control Hosp Epidemiol 1993;14:595–600.Google Scholar
20.Goering, RV, Molecular Epidemiology Study Group. Guidelines for evaluating pulsed-field restriction fragment patterns in the epidemiological analysis of nosocomial infection. Presented at the third International Meeting on Bacterial Epidemiological Markers 1994. Abstract F19.Google Scholar
21.Struelens, MJ, Gerner Smidt, P, Rosdahl, V, et al. Guidelines for appropriate use, evaluation, and interpretation of microbial epidemiological typing systems. Eur J Clin Microbiol Infect Dis. Submitted for publication.Google Scholar
22.Bassam, BJ, Caetano-Anolles, G, Gresshoff, PM. DNA amplification fingerprinting of bacteria. Appl Microbiol Biotechnol 1992;38:70–76.Google Scholar
23.Caetano-Anolles, G, Bassam, BJ, Gresshoff, PM. Primer-template interactions during DNA amplification fingerprinting with single arbitrary oligonucleotides. Mol Gen Genet 1992;235:157–165.Google Scholar
24.van Belkum, A, Kluytmans, J, van Leeuwen, W, et al.Multicenter evaluation of arbitrary primed polymerase chain reaction for typing of Staphylococcus aureus strains. J Clin Microbiol 1995;33:1537–1547.Google Scholar
25.Tenover, FC, Arbeit, RD, Archer, G, et al.Comparison of traditional and molecular methods of typing of Staphylococcus aureus. J Clin Microbiol 1994;32:407–415.Google Scholar