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Lethal Activity of Nonthermal Plasma Sterilization Against Microorganisms

  • Richard A. Venezia (a1), Michael Orrico (a2), Edward Houston (a2), Shu-Min Yin (a2) and Yelena Y. Naumova (a2)...



To determine the range and the mode of germicidal activity of sterilants generated by a nonthermal plasma sterilization system for microorganisms.


Representative bacteria, spores, viruses, bacteriophages, and fungi were exposed to the plasma cycle and the residual viability was measured in vitro. To assess the mode of lethal injury, Escherichia coli, Staphylococcus aureus, Bacillus atrophaeus, and bacteriophages were exposed to the plasma cycle, and the effects of the plasma-generated sterilants on the biological parameters were determined.


There were at least 4-6 log reductions in viability for all microorganisms after 10 minutes of exposure to the plasma cycle. Electron micrographs and studies of the inhibition of bacteriophage infectivity suggested that the primary injury is to the organisms' cell envelopes. The plasma cycle also denatured isolated bacterial proteins and inactivated bacteriophages, but it had no effect on isolated DNA and bacterial proteins within exposed bacteria.


Nonthermal plasma, which is produced at atmospheric temperature and pressure, generates sterilants that kill high concentrations of microorganisms and inactivate viruses during a 10-minute exposure. The primary injury appears to be at the surface structures of the organisms. This suggests that nonthermal plasma has utility for sterilization of heat-sensitive medical materials and devices.


Corresponding author

Department of Pathology, University of Maryland School of Medicine, 22 South Greene Street, Baltimore, Maryland 21201, (


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1.Rutala, WA, Gergen, MF, Weber, DJ. Comparative evaluation of the sporicidal activity of new low-temperature sterilization technologies: ethylene oxide, 2 plasma sterilization systems, and liquid peracetic acid. Am J Infect Control 1998;26:393398.
2.Rutala, WA, Weber, DJ. Infection control: the role of disinfection and sterilization. J Hosp Infect 1999;43(Suppl):S4355.
3.Rutala, WA, Weber, DJ. Clinical effectiveness of low-temperature sterilization technologies. Infect Control Hosp Epidemiol 1998;19:798804.
4.Griffiths, N. Low-temperature sterilization using gas plasmas. Med Device Technol 1993;4:3740.
5.Stoffels, E, Rieft, IE, Sladek, RE, van der Laan, EP, Slaaf, DW. Gas plasma treatment: a new approach to surgery? Crif Rev Biomed Eng 2004;32:427460.
6.Alfa, MJ. Changes in hospital practice. Biomed Instrum Technol 1996;30:400404.
7.Carlo, A. New surgical instruments and sets bring sterilization challenges. Mater Manag Health Care 2006;15:64.
8.Becker, KH, Kogelschatz, U, Schoenbach, KH, Barker, RJ. Non-equilibrium Air Plasmas at Atmospheric Pressure. London: Taylor & Francis; 2004.
9.Moisan, M, Barbeau, J, Moreau, S, Pelletier, J, Tabrizian, M, Yahia, LH. Low-temperature sterilization using gas plasmas: a review of the experiments and an analysis of the inactivation mechanisms. Int J Pharm 2001;226:121.
10.Laroussi, M, Mendis, D, Rosenberg, M. Plasma interaction with microbes. New J Phys 2003;5:41.
11.Laroussi, M, Leipold, F. Evaluation of the roles of reactive species, heat, and UV radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure. Int J Mass Spectrom 2004;233:8186.
12.Kong, M. A complementary sterilisation strategy using cold atmospheric plasmas. Med Device Technol 2006;17:2628.
13.Krebs, MC, Becasse, P, Verjat, D, Darbord, JC. Gas-plasma sterilization: relative efficacy of the hydrogen peroxide phase compared with that of the plasma phase. Int J Pharm 1998;160:7581.
14.Lerouge, S, Wertheimer, MR, Yahia, LH. Plasma sterilization: a review of parameters, mechanisms, and limitations. Plasmas Polym 2001;6:175188.
15.Soloshenko, A, Tsiolko, W, Khomich, VA, et al.Features of sterilization using low-pressure DC-discharge hydrogen peroxide plasma. IEEE Trans Plasma Sci 2002;30:14401444.
16.Menashi, WP. Treatment of surfaces. United States Patent No. 3,383,163. Patented May 14, 1968.
17.Babko-Malyi, S. Biocidal properties of the ambient air plasma: symposium on environmental, biological, and medical applications of microplasmas. In: Program of 2nd International Workshop on Microplasmas; October 6-8, 2004; Stevens Institute of Technology, Hoboken, NJ.
18. ISO 14937:2000 Sterilization of health care products— General requirements for characterization of a sterilizing agent and the development, validation and routine control of a sterilization process for medical devices, 2nd ed. Doc. 198 N762.
19. ISO 14161:2000 Sterilization of healthcare products— Biological indicators— Guidance for the selection, use, and interpretation of results.
20.Gaunt, L, Beggs, C, Georghiou, G. Bactericidal action of the reactive species produced by gas-discharge nonthermal plasma at atmospheric pressure: a review. IEEE Trans Plasma Sci 2006;34:12571269.
21.Driks, A. Bacillus subtilis spore coat Microbiol Mol Biol Rev 1999;63:120.
22.Sharma, A, Pruden, A, Yu, Z, Collins, G. Bacterial inactivation in open air by afterglow plume emitted from a grounded hollow slot electrode. Environ Sci Technol 2005;39:339344.

Lethal Activity of Nonthermal Plasma Sterilization Against Microorganisms

  • Richard A. Venezia (a1), Michael Orrico (a2), Edward Houston (a2), Shu-Min Yin (a2) and Yelena Y. Naumova (a2)...


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