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Inactivation of Animal and Human Prions by Hydrogen Peroxide Gas Plasma Sterilization

Published online by Cambridge University Press:  02 January 2015

C. Rogez-Kreuz ,
R. Yousfi ,
C. Soufflet ,
I. Quadrio ,
Z.-X. Yan ,
V. Huyot ,
C. Aubenque ,
P. Destrez ,
K. Roth  and
C. Roberts
...Show all authors
C. Rogez-Kreuz
Affiliation:
Neurovirology Laboratory, SPI-BIO, CEA, Fontenay aux Roses, France
R. Yousfi
Affiliation:
Neurovirology Laboratory, SPI-BIO, CEA, Fontenay aux Roses, France
C. Soufflet
Affiliation:
Advanced Sterilization Products, Division of Ethicon, Johnson & Johnson, Irvine, California
I. Quadrio
Affiliation:
Neurobiology Department, Centre de Biologie et Pathologie Est, Hospices Civils de Lyon, Bron, France
Z.-X. Yan
Affiliation:
Service fur Medizinprodukte, Tübingen, Germany
V. Huyot
Affiliation:
Neurovirology Laboratory, SPI-BIO, CEA, Fontenay aux Roses, France
C. Aubenque
Affiliation:
Neurovirology Laboratory, SPI-BIO, CEA, Fontenay aux Roses, France
P. Destrez
Affiliation:
Advanced Sterilization Products, Division of Ethicon, Johnson & Johnson, Irvine, California
K. Roth
Affiliation:
Service fur Medizinprodukte, Tübingen, Germany
C. Roberts*
Affiliation:
Advanced Sterilization Products, Division of Ethicon, Johnson & Johnson, Irvine, California
M. Favero
Affiliation:
Advanced Sterilization Products, Division of Ethicon, Johnson & Johnson, Irvine, California
P. Clayette
Affiliation:
Neurovirology Laboratory, SPI-BIO, CEA, Fontenay aux Roses, France
*
33 Technology Dr., Irvine, CA 92618 (crobert2@its.jnj.com)
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Abstract

Prions cause various transmissible spongiform encephalopathies. They are highly resistant to the chemical and physical decontamination and sterilization procedures routinely used in healthcare facilities. The decontamination procedures recommended for the inactivation of prions are often incompatible with the materials used in medical devices. In this study, we evaluated the use of low-temperature hydrogen peroxide gas plasma sterilization systems and other instrument-processing procedures for inactivating human and animal prions. We provide new data concerning the efficacy of hydrogen peroxide against prions from in vitro or in vivo tests, focusing on the following: the efficiency of hydrogen peroxide sterilization and possible interactions with enzymatic or alkaline detergents, differences in the efficiency of this treatment against different prion strains, and the influence of contaminating lipids. We found that gaseous hydrogen peroxide decreased the infectivity of prions and/or the level of the protease-resistant form of the prion protein on different surface materials. However, the efficiency of this treatment depended strongly on the concentration of hydrogen peroxide and the delivery system used in medical devices, because these effects were more pronounced for the new generation of Sterrad technology. The Sterrad NX sterilizer is 100% efficient (0% transmission and no protease-resistant form of the prion protein signal detected on the surface of the material for the mouse-adapted bovine spongiform encephalopathy 6PB1 strain and a variant Creutzfeldt-Jakob disease strain). Thus, gaseous or vaporized hydrogen peroxide efficiently inactivates prions on the surfaces of medical devices.

Type
Review Article
Information
Infection Control & Hospital Epidemiology , Volume 30 , Issue 8 , August 2009 , pp. 769 - 777
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2009

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