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Comparison of Broth and Filtration Methods for Culturing of Intravenous Fluids

Published online by Cambridge University Press:  02 January 2015

Jenice N. Longfield ,
Patricia Charache ,
Earl L. Diamond  and
Timothy R. Townsend
Jenice N. Longfield*
Affiliation:
Hospital Epidemiology Unit and the Microbiology Division of Laboratory Medicine, and the Department of Epidemiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Patricia Charache
Affiliation:
Hospital Epidemiology Unit and the Microbiology Division of Laboratory Medicine, and the Department of Epidemiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Earl L. Diamond
Affiliation:
Hospital Epidemiology Unit and the Microbiology Division of Laboratory Medicine, and the Department of Epidemiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Timothy R. Townsend
Affiliation:
Hospital Epidemiology Unit and the Microbiology Division of Laboratory Medicine, and the Department of Epidemiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
*
Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20014
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Abstract

Ninety-six specimens of intravenous fluid solutions (D5/025 NS) were inoculated with S. aureus, E. coli, P. aeruginosa, K. pneumoniae, E. agglomerans, or C. albicans in concentrations of .1, 1, 10, or 102 organisms/ml. They were cultured in tubes containing 5 ml of double enriched broth and after passage through a .45 μ pore membrane filter. After 24 hours of incubation, broth cultures were 68% as sensitive as the filter cultures (p<.001). At the lowest concentration (.1 organism/ml) broth cultures were only 45% as sensitive as the membrane filter technique after 24 hours of growth (p<.001). Membrane filters provide a rapid method to accurately detect and quantitate the presence of microbial contamination even at very low levels of concentration. The simplicity and accuracy of the filtration method offers the clinician a valuable adjunct in managing suspected cases of intravenous fluid related sepsis.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 3 , Issue 5 , October 1982 , pp. 397 - 400
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1982

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