Skip to main content Accessibility help
×
Home

Comparative in Vitro Activity of Antiseptics and Disinfectants Versus Clinical Isolates of Candida Species

  • Jan Silverman (a1) (a2), Jose A. Vazquez (a2) (a3), Jack D. Sobel (a2) and Marcus J. Zervos (a1) (a2)

Abstract

Objective:

To evaluate the in vitro activity of antiseptics and detergents against Candida.

Design:

One strain each of Candida albicans, Candida tropicalis, Candida lusitaniae, Candida parapsilosis, Candida kefyr, Candida glabrata, and an American Type Culture Collection strain of Escherichia coli (control) were studied. Clinical isolates were obtained from patients in a bone marrow unit of a large tertiary hospital. Antiseptic and disinfectant agents studied were used in the hospital where isolates were identified for cleaning of inanimate surfaces or hand washing. In vitro susceptibility was determined using a broth macrodilution method with exposure times to antiseptic or disinfectant agent of 15 seconds to 4 minutes and concentrations of agents that ranged from undiluted to 1:10,000 dilution.

Setting:

A 900-bed teaching hospital.

Results:

Of disinfectants tested, Vestal and Sparquat inhibited growth of all species at dilutions of ≤1:100 at all contact times for all species. Clorox showed inhibition of growth at 1:100 dilution after 30 seconds of contact time for all isolates. Of antiseptics studied, Hibiclens inhibited growth of all species except C tropicalis at dilutions of ≤1:100 at all contact times and for C tropicalis after 60 seconds. Clinidine inhibited growth of all species at dilutions of ≤1:100 at all contact times for all species with the exception of C glabrata and C tropicalis, which grew at the undiluted concentration. Ultradex failed to demonstrate killing of any species for any dilutions tested.

Conclusions:

The results of this study show varying degrees of in vitro inhibition of growth by a variety of antiseptics and disinfectants against clinical isolates of Candida species from hospitalized patients.

Copyright

Corresponding author

William Beaumont Hospital, 3601 W 13 Mile Rd, Royal Oak, MI 48073

References

Hide All
1. Banerjee, SN, Emori, TG, Culver, DA, Gaynes, RP, Jarvis, WR, Horan, T, et al. Secular trends in nosocomial primary bloodstream infections in the United States. National Nosocomial Infectious Surveillance System. Am J Med 1991;91(suppl 3B):865895.
2. Komshian, SV, Uwaydah, AK, Sobel, JD, Crane, L. Fungemia caused by Candida sp and Torulopsis glabrata in hospitalized patients frequency, characteristics and evaluation of factors influencing outcome. Rev Infect Dis 1989;3:379390.
3. Wey, SB, Motomi, M, Pfaller, MA, Woolsen, RF, Wenzel, RR Hospital acquired candidemia: the attributable mortality and excess length of stay. Arch Intern Med 1988;148:26422647.
4. Vazquez, JA, Sanchez, V, Dmuchowski, C, Dembry, LM, Sobel, JD, Zervos, MJ. Nosocomial acquisition of Candida albicans: an epidemiologic study. J Infect Dis 1993;168:195201.
5. Sanchez, V, Vazquez, J, Jones, DB, Dembry, L, Sobel, JD, Zervos, MJ. Nosocomial acquisition of Candida parapsilosis: an epidemiologic study. Am J Med 1993;94:577582.
6. Sanchez, V, Vazquez, JA, Barth-Jones, D, Dembry, L, Sobel, JD, Zervos, MJ. Epidemiology of nosocomial acquisition of Candida lusitaniae . J Clin Microbiol 1993;30:30053008.
7. Vazquez, JA, Dembry, LM, Sanchez, V, Vazquez, MA, Sobel, JD, Dmuchowski, C, Zervos, MJ. Nosocomial Candida glabrata colonization: an epidemiologic study. J Clin Microbiol 1998;36:421426.
8. Isenberg, HD, Tucci, V, Clintron, F, Sincer, C, Weinstein, GS, Tyras, D. Single source outbreak of Candida tropicalis complicating coronary bypass surgery. J Clin Microbiol 1989;27:24262428.
9. Jarvis, WR Epidemiology of nosocomial fungal infections, with emphasis on Candida species. Clin Infect Dis 1995;20:15261530.
10. Vaudry, WL, Tierney, AJ, Weinman. Investigating of cluster of systemic Candida albicans infections in a neonatal intensive care unit. J Infect Dis 1988;158:13751379.
11. Weems, JJ, Chamberland, ME, Ward, J, Willy, M, Payne, AA, Solomon, SL. Candida parapsilosis fungemia associated with parenteral nutrition and contaminated blood pressure transducers. J Clin Microbiol 1987;1925:10291032.
12. Burnie, JP, Odds, FC, Lee, W, Webster, C, Williams, JD. Outbreak of systemic Candida albicans in an intensive care unit caused by cross infection. BMJ 1985;290:746748.
13. Berkelman, RL, Holland, BW, Anderson, R. Increased bactericidal activity of dilute preparations of Clinidine solutions. J Clin Microbiol 1982;15:635639.
14. Haley, CE, Mailing-Casin, M, Smith, J, Luby, J, Mackowiak, E Bactericidal activity of antiseptics against methicillin resistant Staphylococcus aureus . J Clin Microbiol 1985;21:991992.
15. Russell, AD, Ahonkhai, I, Rogers, DT. Microbiological applications of the inactivation of antibiotics and other antimicrobial agents. J Appi Bacteriol 1979;46:207245.
16. Phelps, M, Ayliffe, GAJ, Babb, JR An outbreak of candidiasis in a special care unit the use of resitogram typing method. J Hosp Infect 1986;7:1320.
17. Maki, DG, Alvarado, CJ, Hassemr, CA, Zilz, MA. Relation of the inanimate hospital environment to endemic nosocomial infection. N Engl J Med 1982;307:15621566.
18. Nicoletti, G, Boghossian, V, Gurevitch, F, Borland, R, Morganroth, P. The antimicrobial activity in vitro of Chlorhexidine, a mixture of isothiazoli-nones (‘Kathon’ CG) and cetyl trimethyl ammonium bromide (CTAB). J Hosp Infect 1993;23:87111.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed