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Bacillus Species Pseudomeningitis

  • Ludwig A. Lettau (a1), Dean Benjamin (a2), H. Furman Cantrell (a2), David W. Potts (a1) and J. Michael Boggs (a1)...

Abstract

A cluster of cerebrospinal fluid Gram's stains showing gram-positive bacilli and of cerebrospinal fluid cultures growing Bacillus species in a large community teaching hospital prompted an epidemiologic and microbiologic investigation. Pseudomeningitis was suspected and confirmed when cultures of uninoculated commercial trypticase soy broth with 5% Fildes enrichment grew Bacillus species. Secondary contamination of the pipettes used for inoculation accounted for the positive cerebrospinal fluid Gram's stains. The costs of this pseudo-outbreak included unnecessary antibiotic therapy, lumbar punctures, and hospitalization. Such adverse effects can be minimized by increased physician awareness of pseudoinfections and by prompt investigation of such occurrences.

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Greenville Memorial Medical Center, 701 Grove Road, Greenville, South Carolina 29605

References

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1.Weinstein, RA. Stamm, WE: Pseudoepidemics in hospital. Lancet 1977; 2:862864.
2.Maki, DG: Through a glass darkly: Nosocomial pseudoepidemics and pseudobacteremias. Arch Intern Med 1980; 140:2028.
3.Musher, DM, Schell, RF: False-positive gram stains of cerebrospinal fluid. Ann Intern Med 1973; 79:603604.
4.Weinstein, RA, Bauer, FW, Hoffman, RD, et al: Factitious meningitis: Diagnostic error due to nonviable bacteria in commercial lumbar puncture trays. JAMA 1975; 233:878879.
5.Ericsson, CD, Carmichael, M, Pickering, LK, et al: Erroneous diagnosis of meningitis due to false-positive gram stains. South Med J 1978; 71:15241525.
6.Jovner, RW, Idriss, ZH, Wilfert, CM: Misinterpretation of cerebrospinal fluid gram stain. Pediatrics 1974; 51:300362.
7.Hoke, CH. Batt, JM. Mirrett, S. et al: False-positive gram-stained smears. JAMA 1979; 241:478480.
8.Centers for Disease Control: False-positive gram stain due to nonviable organisms in sterile commercial transport medium. MMWR 1978: 27:23.
9.Walsh, DM. Eberiel, DT: Pseudomonas cepacia isolated from crystal violet solution in a hospital laboratory. J Clin Microbiol 1986; 23:962.
10.Harris, AA. Pottage, JC. Fliegelman, R, et al: A pseudoepidemic due to Salmonella typhimurium. Diagn Microbiol Infect Dis 1983; 1:335337.
11.Graham, DR, Anderson, RL, Ariel, FE., et al: Epidemic nosocomial meningitis due to Citrobacter diversus in neonates. J Infect Dis 1981: 144:203209.
12.Goossens, H. Henocque, G, Kremp, L, et al: Nosocomial outbreak of Campylobacter jejuni meningitis in newborn infants. Lancet 1980; 2:146149.
13.Crowley, MM, Shannon, R. Spivack, M, et al: Pseudobacteremia due to intrinsic contamination of blood culture media Bacillus species (abstract). Am J Infect Control 1983; 11:150.
14.Noble, RC. Reeves, SA: Bacillus species pseudosepsis caused by contaminated commercial blood culture media. JAMA 1971; 230:10021004.
15.MacDonald, N: Investigation of an outbreak of pseudobacteremia attributed to Bacillus species in a general hospital. Abstracts, 82nd Annual Meeting of the American Society for Microbiology, Atlanta, March 7-12, 1982, p 83.
16.Berger, SA: Pseudobacteremia due to contaminated alcohol swabs. J Clin Microbiol 1983; 18:974975.
17.Gurevich, I. Tafuro, P. Krystofiak, S. et al: Three clusters of Bacillus pseudobacteremia related to a radiometric blood culture analyzer. Infect Control 1984; 5:7174.
18.Goldstein, B, Abrutyn, E: Pseudo-outbreak of Bacillus species: Related to fibreoptic bronchoscope. J Hosp Infect 1985; 6:194200.
19.Tuazon, CU, Murray, HW. Levy, C, et al: Serious infectious from Bacillus sp. JAMA 1979; 241:11371140.
20.Cotton, DJ, Gill, VJ, Marshall, DJ, et al: Clinical features and therapeutic interventions in 17 cases of Bacillus bacteremia in an immunosuppressed patient population. J Clin Microbiol 1987; 25:672674.

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Bacillus Species Pseudomeningitis

  • Ludwig A. Lettau (a1), Dean Benjamin (a2), H. Furman Cantrell (a2), David W. Potts (a1) and J. Michael Boggs (a1)...

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