Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-23T12:55:05.341Z Has data issue: false hasContentIssue false
  • English
  • Français

A third gene affecting GABA transaminase levels in Aspergillans nidulus

Published online by Cambridge University Press:  14 April 2009

Christopher R. Bailey ,
Herbert N. Arst Jr  and
Hugh A. Penfold
Christopher R. Bailey
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, England
Herbert N. Arst Jr
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, England Department of Genetics, Ridley Building, The University, Newcastle upon Tyne NE1 7RU, England
Hugh A. Penfold
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, England
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mutations in the gatB gene as well as mutations in the putative structural gene gatA and the positive acting regulatory gene intA can affect γ-amino-n-butyrate (GABA) transaminase (EC 2.6.1.19) levels in the ascomycete fungus Aspergillus nidulans. Partial or complete loss of function mutations in gatA, gatB and ssuA, which specifies succinic semialdehyde dehydrogenase, can lead to accumulation of ω-amino acids resulting in pseudo-constitutivity and elevated expression of (retained) activities under intA control. These regulatory effects underlie selective methods for gatB−, ssuA− and leaky gatA− mutations. However, all three gatB− alleles which have been selected lead only to partial loss of GABA transaminase activity as judged by both in vivo and in vitro criteria. It has not been established whether the leakiness of these three gatB− mutations is an allele-specific or a locus-specific effect and whether or not the GABA transaminase present in gatB− strains differs from the wild type enzyme. Thus the role of the gatB product remains to be elucidated. The gatB gene is not closely linked to any other gene involved in ω-amino metabolism or related pathways.

Type
Research Article
Information
Genetics Research , Volume 36 , Issue 2 , October 1980 , pp. 167 - 180
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Alderson, T. & Hartley, M. J. (1969). Specificity for spontaneous and induced forward mutation at several gene loci in Aspregillus nidulans. Mutation Research 8, 255–264.CrossRefGoogle Scholar
Arst, H. N. Jr (1976). Integrator gene in Aspergillus nidulans. Nature 262, 231–234.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr (1977). Some genetical aspects of ornithine metabolism in Aspergillus nidulans. Molecular and General Genetics 151, 105–110.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr (1978). GABA transaminase provides an alternative route of β-alanine synthesis in Aspergillus nidulans. Molecular and General Genetics 163, 23–27.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr & Bailey, C. R. (1977). The regulation of carbon metabolism in Aspergillus nidulans. In Genetics and Physiology of Aspergillus (ed. Smith, J. E. and Pateman, J. A.), pp. 131–146. London: Academic Press.Google ScholarPubMed
Arst, H. N. JrBailey, C. R. & Penfold, H. A. (1980). A possible rôle for acid phosphatase in γ-amino-n-butyrate uptake in Aspergillus nidulans. Archives of Microbiology 125, 153–158.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr & Cove, D. J. (1969). Methylammonium resistance in Aspergillus nidulans. Journal of Bacteriology 98, 1284–1293.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr & Cove, D. J. (1973). Nitrogen metabolite repression in Aspergillus nidulans. Molecular and General Genetics 126, 111–141.CrossRefGoogle ScholarPubMed
Arst, H. N. JrPenfold, H. A. & Bailey, C. R. (1978). Lactam utilization in Aspergillus nidulans: evidence for a fourth gene under the control of the integrator gene intA. Molecular and General Genetics 166, 321–327.CrossRefGoogle ScholarPubMed
Arst, H. N. JrRand, K. N. & Bailey, C. R. (1979). Do the tightly linked structural genes for nitrate and nitrite reductases in Aspergillus nidulans form an operon? Evidence from an insertional translocation which separates them. Molecular and General Genetics 174, 89–100.CrossRefGoogle Scholar
Bailey, C. & Arst, H. N. Jr (1975). Carbon catabolite repression in Aspergillus nidulans. European Journal of Biochemistry 51, 573–577.CrossRefGoogle Scholar
Bailey, C. R., Penfold, H. A. & Arst, H. N. Jr (1979). Cis-dominant regulatory mutations affecting the expression of GABA permease in Aspergillus nidulans. Molecular and General Genetics 169, 79–83.CrossRefGoogle ScholarPubMed
Britten, R. J. & Davidson, E. H. (1969). Gene regulation for higher cells: a theory. Science 165, 349–357.CrossRefGoogle ScholarPubMed
Clutterbuck, A. J. (1974). Aspergillus nidulans. In Handbook of Genetics, vol. I (ed. King, R. C.), pp. 447–510. New York: Plenum Press.Google Scholar
Clutterbuck, A. J. & Cove, D. J. The genetic loci of Aspergillus nidulans. In GRC Handbook of Microbiology, vol. II, 2nd ed. (ed. Laskin, A. I. and Lechevalier, H.). West Palm Beach, Florida: Chemical Rubber Co. (In the press.)Google Scholar
Cove, D. J. (1966). The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et Biophysica Acta 113, 51–56.CrossRefGoogle ScholarPubMed
Gunatilleke, I. A. U. N., Arst, H. N. Jr & Scazzocchio, C. (1975). Three genes determine the carboxin sensitivity of mitochondrial succinate oxidation in Aspergillus nidulans. Genetical Research 26, 297–305.CrossRefGoogle ScholarPubMed
Hynes, M. J. (1973 a). Pleiotropic mutants affecting the control of nitrogen metabolism in Aspergillus nidulans. Molecular and General Genetics 125, 99–107.CrossRefGoogle ScholarPubMed
Hynes, M. J. (1973 b). Alterations in the control of glutamate uptake in mutants of Aspergillus nidulans. Biochemical and Biophysical Research Communications 54, 685–689.CrossRefGoogle ScholarPubMed
Hynes, M. J. (1975). Studies on the role of the areA gene in the regulation of nitrogen catabolism in Aspergillus nidulans. Australian Journal of Biological Sciences 28, 301–313.CrossRefGoogle ScholarPubMed
Hynes, M. J. (1978). Multiple independent control mechanisms affecting the acetamidase of Aspergillus nidulans. Molecular and General Genetics 161, 59–65.CrossRefGoogle ScholarPubMed
Hynes, M. J. (1979). Fine-structure mapping of the acetamidase structural gene and its controlling region in Aspergillus nidulans. Genetics 91, 381–392.CrossRefGoogle ScholarPubMed
Hynes, M. J. & Pateman, J. A. J. (1970). The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. I. Mutants able to utilize acrylamide. Molecular and General Genetics 108, 97–106.CrossRefGoogle ScholarPubMed
McCully, K. S. & Forbes, E. (1965). The use of p-fluorophenylalanine with ‘master strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6, 352–359,CrossRefGoogle ScholarPubMed
Penfold, H. A. (1978). Omega-amino acid catabolism in Aspergillus nidulans. Ph.D. thesis. University of Cambridge.Google Scholar
Pontecorvo, G., Roper, J. A., Hemmons, L. M., MacDonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141–238.CrossRefGoogle ScholarPubMed
Rand, K. N. (1978). Aspects of the control of nitrogen metabolism in Aspergillus nidulans. Ph.D. thesis, University of Cambridge.Google Scholar
Rand, K. N. & Arst, H. N. Jr (1977). A mutation in Aspergillus nidulans which affects the regulation of nitrite reductase and is tightly linked to its structural gene. Molecular and General Genetics 155, 67–75.CrossRefGoogle ScholarPubMed