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Strain variation in spermatozoal glycosidases in inbred mice

Published online by Cambridge University Press:  14 April 2009

Steven J. Self ,
Bryan G. Winchester  and
James R. Archer
Steven J. Self
Affiliation:
Department of Biochemistry, Queen Elizabeth College (University of London), Campden Hill Road, London W8 7AH G. D. Searle & Co. Ltd., Research Division, P.O. Box 53, Lane End Road, High Wycombe, Bucks. HP12 4HL
Bryan G. Winchester
Affiliation:
Department of Biochemistry, Queen Elizabeth College (University of London), Campden Hill Road, London W8 7AH
James R. Archer
Affiliation:
G. D. Searle & Co. Ltd., Research Division, P.O. Box 53, Lane End Road, High Wycombe, Bucks. HP12 4HL

Summary

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Ten glycosidases were measured in suspensions of spermatozoa from the vasa deferentia of two inbred mouse strains and their intercrosses. Eight of these glycosidases were associated with the sperm cells and all of these showed genetical variation between the strains except α-l-fucosidase with optimal activity at pH 5·4. In contrast liver enzyme activities showed no significant variation except α-l-fucosidase. Genetic studies indicated that the variation of spermatozoal β-d-hexosaminidase, α-d-mannosidase, α-l-fucosidase and β-d-galactosidase are inherited at autosomal loci and α-d-galactosidase variation shows X-linked inheritance. We propose a new provisional gene symbol (Afuc-2) for a spermatozoal variant of α-l-fucosidase.

Type
Research Article
Information
Genetics Research , Volume 32 , Issue 2 , October 1978 , pp. 183 - 193
Copyright
Copyright © Cambridge University Press 1978

References

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