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On the localization of a factor responsible for host-controlled restriction in Escherichia coli K(P1)

Published online by Cambridge University Press:  14 April 2009

J. Schell
Affiliation:
Laboratory for Microbiology, State University Ghent, Belgium
S. W. Glover
Affiliation:
M.R.C. Microbial Genetics Research Unit Hammersmith Hospital, Ducane Road, London, W.12
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The restriction of phage λ.C by K(P1) cells is reduced when the cells are subjected to an EDTA cold-wash treatment which has been shown to remove surface-localized enzymes. We conclude that a surface-localized enzyme plays an essential role in host-controlled restriction.

Type
Short Papers
Copyright
Copyright © Cambridge University Press 1966

References

REFERENCES

Adams, M. H. (1950). Methods for the study of bacterial viruses. In Methods in Medical Research (Conrose, J. H., ed.), vol. 2, pp. 173. Chicago: The Year Book Publishers, Inc.Google Scholar
Arber, W. (1958). Transduction des caractères Gal par le bacteriophage μ. Archs. Sci., Geneve, 11, 259330.Google Scholar
Arber, W. (1960). Polylysogeny for bacteriophage lambda. Virology, 11, 250272.CrossRefGoogle Scholar
Arber, W. & Dussoix, D. (1962). Host specificity of DNA produced by Escherichia coli I. Host controlled modification of bacteriophage μ. J. molec. Biol. 5, 1836.CrossRefGoogle Scholar
Cordonnter, C. & Bernardi, G. (1965). Localisation of E. coli endonuclease I. Biochem. biophys. Res. Commun. 20, 555559.CrossRefGoogle Scholar
Fukasawa, T. (1964). The course of infection with abnormal bacteriophage T4 containing non-glucosylated DNA on Escherichia coli strains. J. molec. Biol. 9, 525536.CrossRefGoogle ScholarPubMed
Molholt, B. & Fraser, D. (1965). Reversal of the restriction for host modified T2 and T4 DNA upon conversion of non-permissive Escherichia coli to spheroplasts. Biochem. biophys. Res. Commun. 19, 571575.CrossRefGoogle ScholarPubMed
Neu, H. C. & Heppel, L. A. (1964 a). The release of ribonuclease into the medium when Escherichia coli cells are converted to spheroplasts. J. biol. Chem. 239, 3893.CrossRefGoogle Scholar
Neu, H. C. & Heppel, L. A. (1964 b). On the surface localisation of enzymes in Escherichia coli. Biochem. biophys. Res. Commun. 17, 215219.CrossRefGoogle Scholar
Schell, J. & Glover, S. W. (1966 a). The effect of heat on host-controlled restriction in Escherichia coli K(P1). J. gen. Microbiol. (in press).Google Scholar
Schell, J. & Glover, S. W. (1966 b). The effect of various physiological conditions on host-controlled restriction in Escherichia coli K(P1). Genet. Res. 7, 273276.CrossRefGoogle ScholarPubMed