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Mutants of Aspergillus nidulans unable to use choline-0-sulphate

Published online by Cambridge University Press:  14 April 2009

Herbert N. Arst Jr
Affiliation:
Department of Genetics, Milton Road, Cambridge, CB 41 XH, England

Summary

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Mutants of Aspergillus nidulans unable to use either the choline moiety or the sulphate moiety of exogenous choline-0-sulphate have been selected. Choline-0-sulphate non-utilizing (csu) mutations have no other apparent pleiotropic effects, but it has not yet been established whether they lead to loss of choline sulphatase (and thus of the ability to utilize endogenously produced choline-0-sulphate) or to loss of a specific transport system for choline-0-sulphate or to loss of both.

Type
Short Papers
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Adelberg, E. A., Mandel, M. & Chen, G. C. C. (1965). Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18, 788795.CrossRefGoogle Scholar
Arst, H. N. Jr., (1968). Genetic analysis of the first steps of sulphate metabolism in Aspergillus nidulans. Nature 219, 268270.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr., MacDonald, D. W. & Cove, D. J. (1970). Molybdate metabolism in Aspergillus nidulans I. Mutations affecting nitrate reductase and/or xanthine dehydrogenase. Molecular and General Genetics 108, 129145.CrossRefGoogle ScholarPubMed
Bellenger, N., Nissen, P., Wood, T. C. & Segel, I. H. (1968). Specificity and control of choline-0-sulfate transport in filamentous fungi. Journal of Bacteriology 96, 15741585.CrossRefGoogle ScholarPubMed
Cove, D. J. (1966). The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et Biophysica Acta 113, 5156.CrossRefGoogle ScholarPubMed
Darlington, A. J. & Scazzocchio, C. (1967). The use of analogues and the substrate-sensitivity of mutants in analysis of purine uptake and breakdown in Aspergillus nidulans. Journal of Bacteriology 93, 937940.CrossRefGoogle ScholarPubMed
De Flines, J. (1955). The occurrence of a sulfuric acid ester of choline in the mycelium of a strain of Penicillium chrysogenum. Journal of the American Chemical Society 77, 16761677.CrossRefGoogle Scholar
Dorn, G. L. (1967). A revised map of the eight linkage groups of Aspergillus nidulans. Genetics 56, 619631.CrossRefGoogle ScholarPubMed
Gravel, R. A., Käfer, E., Niklewicz-Borkenhagen, A. & Zambryski, P. (1970). Genetic and accumulation studies in sulfite-requiring mutants of Aspergillus nidulans. Canadian Journal of Genetics and Cytology 12, 831840.Google Scholar
Harada, T. & Spencer, B. (1960). Choline sulphate in fungi. Journal of General Microbiology 22, 520527.CrossRefGoogle ScholarPubMed
Hussey, C., Orsi, B. A., Scott, J. & Spencer, B. (1965). Mechanism of choline sulphate utilization in fungi. Nature 207, 632633.CrossRefGoogle ScholarPubMed
Hussey, C. & Spencer, B. (1967). Regulation of arylsulphatase synthesis in Aspergillus nidulans. Biochemical Journal 103, 56P57P.Google Scholar
Itahashi, M. (1961). Comparative biochemistry of choline sulfate metabolism. Journal of Biochemistry (Tokyo) 50, 5261.CrossRefGoogle ScholarPubMed
Lindberg, B. (1955 a). Studies on the chemistry of lichens. VIII. Investigation of a Dermatocarpon and some Roccella species. Acta chemica scandinavica 9, 917919.CrossRefGoogle Scholar
Lindberg, B. (1955 b). Methylated taurines and choline sulphate in red algae. Acta chemica scandinavica 9, 13231326.CrossRefGoogle Scholar
McCully, K. S. & Forbes, E. (1965). The use of p–fluorophenylalanine with ‘master strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6, 352359.CrossRefGoogle ScholarPubMed
Mackintosh, M. E. & Pritchard, R. A. (1963). The production and replica plating of micro-colonies of Aspergillus nidulans. Genetical Research 4, 320322.CrossRefGoogle Scholar
Marzluff, G. A. (1970 a). Genetic and biochemical studies of distinct sulfate permease species in different developmental stages of Neurospora crassa. Archives of Biochemistry and Biophysics 138, 254263.CrossRefGoogle Scholar
Marzluff, G. A. (1970 b). Genetic and metabolic controls for sulfate metabolism in Neurospora crassa: isolation and study of chromate-resistant and sulfate transport-negative mutants. Journal of Bacteriology 102, 716721.CrossRefGoogle Scholar
Marzluff, G. A. & Metzenberg, R. L. (1968). Positive control by the cys-3 locus in regulation of sulfur metabolism in Neurospora. Journal of Molecular Biology 33, 423437.CrossRefGoogle Scholar
Metzenberg, R. L. & Parson, J. W. (1966). Altered repression of some enzymes of sulfur utilization in a temperature-conditional lethal mutant of Neurospora. Proceedings of the National Academy of Sciences of the United States of America 55, 629635.CrossRefGoogle Scholar
Murray, N. E. (1965). Cysteine mutant strains of Neurospora. Genetics 52, 801808.CrossRefGoogle ScholarPubMed
Nissen, P. & Benson, A. A. (1961). Choline sulfate in higher plants. Science 134, 1759.Google Scholar
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141238.Google Scholar
Scott, J. M. & Spencer, B. (1968). Regulation of choline sulphatase synthesis and activity in Aspergillus nidulans. Biochemical Journal 106, 471477.CrossRefGoogle ScholarPubMed
Siddiqi, O., Apte, B. N. & Pitale, M. P. (1966). Genetic regulation of aryl sulphatases in Aspergillus nidulans. Cold Spring Harbor Symposia on Quantitative Biology 31, 381382.CrossRefGoogle Scholar
Spencer, B. & Harada, T. (1960). The role of choline sulphate in sulphur metabolism of fungi. Biochemical Journal 77, 305315.CrossRefGoogle ScholarPubMed
Spencer, B., Hussey, E. C., Orsi, B. A. & Scott, J. M. (1968). Mechanism of choline-0-sulphate utilization in fungi. Biochemical Journal 106, 461469.CrossRefGoogle ScholarPubMed
Stevens, C. M. & Vohra, P. (1955). Occurrence of choline sulfate in Penicillium chrysogenum. Journal of the American Chemical Society 77, 49354936.Google Scholar
Takebe, I. (1960). Choline sulfate as a major soluble sulfur component of conidiospores of Aspergillus niger. Journal of General and Applied Microbiology 6, 8389.Google Scholar
Woolley, D. W. & Peterson, W. H. (1937). The chemistry of mold tissue. XIV. Isolation of cyclic choline sulfate from Aspergillus sydowi. Journal of Biological Chemistry 122, 213218.CrossRefGoogle Scholar