Hostname: page-component-7479d7b7d-q6k6v Total loading time: 0 Render date: 2024-07-10T14:34:41.415Z Has data issue: false hasContentIssue false
  • English
  • Français

Mutagenesis with light and proflavine in phage T4 II. Properties of the mutants

Published online by Cambridge University Press:  14 April 2009

D. A. Ritchie
D. A. Ritchie
Affiliation:
MRC Microbial Genetics Research Unit, Hammersmith Hospital, London, W.12

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In summary, rII mutations induced by exposure of T4 phage particles to either visible light or proflavine are acridine revertible. In both cases mutation occurs without inactivation. The rII mutations induced by the photodynamic action of proflavine on T4 phage particles are equally divided between the acridine revertible and base analogue revertible classes. It is proposed that photodynamic action induces base analogue mutations and that the acridine revertible category arises from the separate effects of light and proflavine.

Type
Short Notes
Information
Genetics Research , Volume 6 , Issue 3 , November 1965 , pp. 474 - 478
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Benzer, S. (1955). Fine structure of a genetic region in bacteriophage. Proc. natn. Acad. Sci. U.S.A. 41, 344–354.Google Scholar
Benzer, S. (1957). The elementary units of heredity, in The Chemical Basis of Heredity (McElroy, W. D. & Glass, B., eds.), pp. 70–93. The Johns Hopkins Press.Google Scholar
Brenner, S., Barnett, L., Crick, F. H. C. & Orgel, A. (1961). The theory of mutagenesis. J. molec. Biol. 3, 121–124.CrossRefGoogle Scholar
Benzer, S. & Freese, E. (1958). Induction of specific mutations with 5-bromouracil. Proc. natn. Acad. Sci. U.S.A. 44, 112–119.CrossRefGoogle ScholarPubMed
Brenner, S., Benzer, S. & Barnett, L. (1958). Distribution of proflavin-induced mutations in the genetic fine structure. Nature, Lond. 182, 983–985.Google Scholar
Crick, F. H. C., Barnett, L., Brenner, S. & Watts-Tobin, R. J. (1961). General nature of the genetic code for proteins. Nature, Lond. 912, 1227–1232.CrossRefGoogle Scholar
De Mars, R. I. (1953). Chemical mutagenesis in bacteriophage T2. Nature, Lond. 172, 964.Google Scholar
Drake, J. W. (1963). Properties of ultraviolet-induced rII mutants of bacteriophage T4. J. molec. Biol. 6, 268–283.CrossRefGoogle Scholar
Freese, E. (1959 a). The specific mutagenic effect of base-analogues on phage T4. J. molec. Biol. 1, 87–105.CrossRefGoogle Scholar
Freese, E. (1959 b). The difference between spontaneous and base-analogue induced mutations of phage T4. Proc. natn. Acad. Sci. U.S.A. 45, 622–633.CrossRefGoogle ScholarPubMed
Hershey, A. D. & Chase, M. (1952). Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. gen. Physiol. 36, 39–56.CrossRefGoogle ScholarPubMed
Kaufman, E. & Hiatt, C. W. (1959). Photodynamic action of proflavine on T2 coliphage. Virology, 9, 478–479.CrossRefGoogle ScholarPubMed
McFall, E. & Stent, G. S. (1958). Three star mutants of coliphage T2. J. gen. Microbiol. 18, 346–363.CrossRefGoogle ScholarPubMed
Nakai, S. & Saeki, T. (1964). Induction of mutations by photodynamic action in Escherichia coli. Genet. Res. 5, 158–161.CrossRefGoogle Scholar
Ritchie, D. A. (1964). Mutagenesis with light and proflavine in phage T4. Genet. Res. 5, 168–169.CrossRefGoogle Scholar
Simon, M. I. & Van Vunakis, H. (1962). The photodynamic reaction of methylene blue with deoxyribonucleic acid. J. molec. Biol. 4, 488–499.CrossRefGoogle ScholarPubMed
Susman, M., Piechowski, M. M. & Ritchie, D. A. (1965). Studies on phage development. I. An acridine-sensitive clock. Virology, 26, 163–174.CrossRefGoogle ScholarPubMed
Symonds, N. & McCloy, E. W. (1958). The irradiation of phage-infected bacteria: Its bearing on the relationship between functional and genetic radiation damage. Virology, 6, 649–668.Google Scholar
Webb, R. B. & Kubitschek, H. E. (1963). Mutagenic and antimutagenic effects of acridine orange in Escherichia coli. Biochem. biophys. Res. Commun. 13, 90–94.CrossRefGoogle Scholar
Welsh, J. N. & Adams, M. H. (1954). Photodynamic inactivation of bacteriophage. J. Bact. 68, 122–127.CrossRefGoogle ScholarPubMed
Yamomoto, N. (1958). Photodynamic inactivation of bacteriophage and its inhibition. J. Bact. 75, 443–448.CrossRefGoogle Scholar