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Meiotic imbalance in laboratory-produced hybrid males of Chorthippus parallelus parallelus and Chorthippus parallelus erythropus

Published online by Cambridge University Press:  14 April 2009

J. L. Bella ,
G. M. Hewitt  and
J. Gosálvez
J. L. Bella*
Affiliation:
Departamento de Biología C-XV. Unidad de Genética, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK
G. M. Hewitt
Affiliation:
School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK
J. Gosálvez
Affiliation:
Departamento de Biología C-XV. Unidad de Genética, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain
*
* Corresponding author.

Summary

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The grasshopper Chorthippus parallelus has two quite distinct subspecies, which meet along the Pyrenees forming a hybrid zone. Using silver staining we show that on the French side Cp. parallelus has three nucleolar organizer regions, on the L2, L3 and X chromosomes, while on the Spanish side Cp. erythropus has only two NORs, on the L2 and L3. Laboratory F1 hybrid males show reciprocal differences in the expression of NORs. When a Cp. erythropus is female parent the male progeny show four active NORs in mitotic cells and two silver precipitates in meiotic cells, as expected. But when a Cp. parallelus female donates the X with a NOR, her male offspring have a variable disrupted nucleolar expression. Some NORs are not expressed and extra sites of cryptic rDNA are revealed. Meiosis is more disturbed in this latter F1 cross with higher levels of polyploidy, but both Fls show around 90% spermatid abnormality. Such variation in rDNA expression is also found in individuals collected from the hybrid zone, and the role of this disturbance in affecting fitness is discussed.

Type
Research Article
Information
Genetics Research , Volume 56 , Issue 1 , August 1990 , pp. 43 - 48
Copyright
Copyright © Cambridge University Press 1990

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