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Low rates of proviral integration in SWR/J–RF/J hybrid mice
Published online by Cambridge University Press: 14 April 2009
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A high frequency of proviral acquisition has previously been reported in the offspring of SWR/J–RF/J hybrid mice. In the present study, it was investigated whether this proviral acquisition would be useful for large-scale insertional mutagenesis studies. A population of SWR/J–RF/J hybrid mice with a predominantly SWR/J background was created. Lines of mice with such a background and partially congenic for two active proviruses from the RF/J strain were generated (the insert lines). Control lines were derived from mice which had no proviral loci but had an otherwise similar genetic background. DNA samples of mice in the insert lines were screened for the appearance of new proviral loci by Southern hybridization. The rate of proviral acquisition, calculated from the observed number of new proviral loci was 0·023 new proviruses per mouse. This rate is lower than found in previous studies and too low for large-scale insertional mutagenesis studies. A sensitivity experiment indicated that there was adequate detection of new proviral loci.The number of segregating proviruses was consistent with the number of newly acquired proviruses actually detected. Two additional crosses between mice in the insert lines and SWR/J mice were performed. The rate of proviral acquisition was greatly increased when SWR/J females were initially mated to insert mice, but remained unchanged when SWR/J males were used. This suggested that mice in the insert lines had acquired a maternally transmitted factor, which was suppressing viral expression and thus reducing the rate of proviral acquisition.
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References
Bautch, V. L. (1986). Genetic background affects integration frequency of ecotropic proviral sequences into the mouse germ line. Journal of Virology 60, 693–701.CrossRefGoogle ScholarPubMed
Buchberg, A. M., Taylor, B. A., Jenkins, N. A., & Copeland, N. G. (1986). Chromosomal localization of Emv-16 and Emv-16, two closely linked ecotropic proviruses of RF/J mice. Journal of Virology 60, 1175–1178.CrossRefGoogle ScholarPubMed
Church, G. M., & Gilbert, W. (1984). Genomic Sequencing. Proceedings of National Academy of Science USA 81, 1991–1995.CrossRefGoogle ScholarPubMed
Duran-Reynals, M. L., (1985). Maternal transmission of resistance to virus-induced leukemia. Progress in Medical Virology 32, 47–57.Google ScholarPubMed
Feinberg, A. P., & Vogelstein, B., (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Biochemistry 132, 6.Google ScholarPubMed
Gridley, T., Soriano, P., & Jaenisch, R., (1987). Insertional mutagenesis in mice. Trends in Genetics 3, 162–166.CrossRefGoogle Scholar
Jenkins, N. A., Copeland, N. G., Taylor, B. A., & Lee, B. K., (1982). Organisation, distribution and stability of endogenous ecotropic murine leukemia virus sequences in chromosomes of Mus musculus. Journal of Virology 43, 26–36.CrossRefGoogle ScholarPubMed
Jenkins, N. A., & Copeland, N. G., (1985). High frequency germline acquisition of ecotropic MuLV proviruses in SWR/J-RF/J hybrid mice. Cell 43, 811–819.CrossRefGoogle ScholarPubMed
Jolicoeur, P., (1979). The Fv-1 gene of the mouse and its control of murine leukemia virus replication. Current Topics in Microbiological Immunology 86, 67–122.CrossRefGoogle ScholarPubMed
Keightley, P. D., Evans, M. J., & Hill, W. G., (1993). Effects of retroviral insertions on quantitative traits in mice. Genetics 135, 1099–1106.CrossRefGoogle Scholar
Lilly, F., & Duran-Reynals, M. L., (1985). Genetic factors in susceptibility to viral leukemogenesis. Progress in Medical Virology 32, 39–46.Google ScholarPubMed
Lock, L. F., Keshet, E., Gilbert, D. J., Jenkins, N. A., & Copeland, N. G., (1988). Studies of the mechanism of spontaneous germline ecotropic provirus acquisition in mice. EMBO Journal 7, 4169–4177.CrossRefGoogle ScholarPubMed
Mayer, A., Duran-Struuck, F., Duran-Reynals, M., & Lilly, F., (1980). Maternally transmitted resistance to lymphoma development in mice of reciprocal crosses of the RF/J and AKR/J strains. Cell 19, 431–436.CrossRefGoogle ScholarPubMed
Melamedoft, M., Lilly, F., & Duran-Reynals, M. L., (1983). Suppression of endogenous murine leukemia virus by maternal resistance factor. Journal of Experimental Medicine 158, 506–514.CrossRefGoogle Scholar
Mackay, T. F. C, Lyman, R. F., & Jackson, M. S., (1992). Effects of P element insertions on quantitative traits in Drosphila melanogaster. Genetics 130, 315–332.CrossRefGoogle Scholar
Panthier, J.-J., & Codamine, H., (1987). Expression of ecotropic MuLV in ovaries of SWR/J-RF/J hybrid mice. Annales Institute Pasteur/Virology 138, 409–422.CrossRefGoogle Scholar
Scharw, H., Ihle, J. N., Wecker, E., Fischinger, P. J., Thiel, H.-J., Bolognesi, D. P., & Schafter, W., (1981). Properties of mouse leukemia viruses. XVII. Factors required for successful treatment of spontaneous AKR leukemia by antibodies against gp71. Virology 111, 568–578.Google Scholar
Snedecor, G. W., & Cochran, W. G., (1989). Statistical methods (Eighth Edition). Iowa State University Press.Google Scholar
Spence, S. E., Gilbert, D. J., Swing, D. A., Copeland, N. G., & Jenkins, N. A., (1989). Spontaneous germ line virus infection and retroviral insertional mutagenesis in eighteen transgenic Srev lines of mice. Molecular and Cellular Biology 9, 177–184.Google ScholarPubMed
Steffen, D. L., Taylor, B. A., & Weinberg, R. A., (1982). Continuing germline integration of AKR proviruses during the inbreeding of AKR mice and derivative recombinant inbred strains. Journal of Virology 42, 165–175.CrossRefGoogle Scholar
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