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Loci of radiation sensitivity in Bs strains of Escherichia coli*

Published online by Cambridge University Press:  14 April 2009

John Donch  and
Joseph Greenberg
John Donch
Affiliation:
Palo Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, California 94301
Joseph Greenberg
Affiliation:
Palo Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, California 94301

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The genes responsible for u.v. sensitivity in ten sensitive mutants of E. coli strain B (Bs strains of R. Hill) have been mapped by transduction. The uvr genes of all the mutants able to reactivate u.v.-irradiated phage (HCR+), including Bs2, 4, 5, 6, 7, 9 and 10, were linked, 50–95%, with malB. The gene of Bs12 (HCR) was also linked to malB as is uvr A. The gene of Bs8 (HCR) was linked to gal, that of Bs3 (HCR) to his. Transduction of mal+ from a strain with uvr A, or a mal+ derivative of Bs12, to Bs2, 4, 5, 6, 7, 9 or 10, yielded about 30% u.v.-resistant transductants. A mal+ transduction with Bs2 mal+ as donor and other Bs strains as recipients yielded < 0·1% u.v.-resistant transductants. The malB-linked uvr genes of all mutants (except Bs3 and Bs8) were transducible with metA. The quasi-reciprocal crosses and three-point tests suggested the order of markers as metA malB uvr (HCR+) uvr (HCR). The sensitivity gene of Bs9 was exceptional in that it appeared to lie between metA and malB. The sensitivity gene of Bs11 could not be mapped because transductants to that strain were not possible, nor did it act as a recipient in sexual recombinations.

Type
Research Article
Information
Genetics Research , Volume 11 , Issue 2 , April 1968 , pp. 183 - 191
Copyright
Copyright © Cambridge University Press 1968

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