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The inhibitory action of transfer factors on lysis of Escherichia coli K 12 by phages μ 2 and Φ 2

Published online by Cambridge University Press:  14 April 2009

J. S. Pitton  and
E. S. Anderson
J. S. Pitton
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London N.W.9
E. S. Anderson
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London N.W.9
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Summary

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A selection of fi+ resistance factors and transfer factors, when introduced into K 12F+, showed a range of inhibitory activity of lysis by the male-specific phage μ 2. This range can be used to subdivide the fi+ factors into fi+1, fi+2, fi+3 and fi+4 classes, according to the degree of inhibition of μ 2 lysis. To this subdivision can be added restriction of the ‘female-specific’ phage φ 2.

Introduction of all the fi+ factors tested into K 12 HfrH totally inhibited lysis by μ 2 in spot tests, but with two fi+1 and one fi+2 factors visible lysis was obtained in agar-layer tests. These three factors caused least inhibition of transfer of pro by HfrH. It can be assumed that both tests reflect lower inhibition of sex fimbrial formation by these fi+ factors than by the remainder.

The fi− factors, when introduced into K 12, can be subdivided on the basis of restrictive effects on phage φ 2.

These effects can be added to phage restriction in the salmonellae for the purposes of further classification of the transfer factors and R-factors.

Type
Research Article
Information
Genetics Research , Volume 16 , Issue 2 , October 1970 , pp. 215 - 224
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. London and New York: Interscience Publishers Inc.CrossRefGoogle Scholar
Anderson, E. S. (1965 a). Origin of transferable drug-resistance factors in Enterobacteriaceae. British Medical Journal ii, 1289–1291.CrossRefGoogle Scholar
Anderson, E. S. (1965 b). A rapid screening test for transfer factors in drug-sensitive Enterobacteriaceae. Nature 208, 1016–1017.CrossRefGoogle ScholarPubMed
Anderson, E. S. (1966). Influence of the Δ transfer factor on the phage sensitivity of Salmonellae. Nature 212, 795–799.CrossRefGoogle ScholarPubMed
Anderson, E. S. & Lewis, M. J. (1965 a). Drug resistance and its transfer in Salmonella typhimurium. Nature 206, 579–583.CrossRefGoogle ScholarPubMed
Anderson, E. S. & Lewis, M. J. (1965 b). Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature 208, 843–849.CrossRefGoogle ScholarPubMed
Clowes, R. C. & Hayes, W. (1968). Experiments in Microbiol Genetics. Oxford and Edinburgh: Blackwell Scientific Publications.Google Scholar
Cuzin, F. (1965). Un bactériophage spécifique du type sexuel F- d' Escherichia coli k 12. Comptes Rendus Académie des Sciences, Paris 260, 6482–6485.Google Scholar
Dettori, R., Maccacaro, G. A. & Piccinin, G. L. (1961). Sex-specific bacteriophages of Escherichia coli K 12. Giornale di Microbiologia 9, 141–150.Google Scholar
Hayes, W. (1953). The mechanism of genetic recombination in Escherichia coli. Cold Spring Harbour Symposium on quantitative Biology 8, 75–83.CrossRefGoogle Scholar
Meynell, E. & Datta, N. (1966). The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli K 12. Genetical Research 7, 134–140.CrossRefGoogle Scholar
Watanabe, T. & Fukasawa, T. (1962). Episome-mediated transfer of drug resistance in Enterobacteriaceae. IV. Interactions between resistance transfer factor and F-factor in Eecherichia coli K 12. Journal of Bacteriology 83, 727–735.CrossRefGoogle Scholar
Watanabe, T., Fukasawa, T. & Takano, T. (1962). Con version of male bacteria of Escherichia coli K 12 to resistance to f phages by infection with the episome ‘Resistance Transfer Factor’. Virology 17, 217–219.CrossRefGoogle Scholar
Watanabe, T., Nishida, H., Ogata, C., Arai, T. & Sato, S. (1964). Episome-mediated transfer of drug resistance in Enterobacteriaceae. VII. Two types of naturally occurring R factors. Journal of Bacteriology 88, 716–726.CrossRefGoogle ScholarPubMed