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Induction of recessive lethal mutations in the T/t–H-2 region of the mouse genome by a point mutagen1

Published online by Cambridge University Press:  14 April 2009

Alexandra Shedlovsky ,
Jean-Louis Guenet ,
Lawrence L. Johnson  and
William F. Dove
Alexandra Shedlovsky
Affiliation:
McArdle Laboratory for Cancer Research and Laboratory of Genetics, University of Wisconsin, Madison, W1 53706U.S.A.
Jean-Louis Guenet
Affiliation:
Génétique des Mammifères, Institut Pasteur, 25, rue du Docteur Roux, 75015 Paris, France
Lawrence L. Johnson
Affiliation:
McArdle Laboratory for Cancer Research and Laboratory of Genetics, University of Wisconsin, Madison, W1 53706U.S.A.
William F. Dove
Affiliation:
McArdle Laboratory for Cancer Research and Laboratory of Genetics, University of Wisconsin, Madison, W1 53706U.S.A.
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Summary

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The T/t–H-2 region on mouse chromosome 17 is known from complex natural variants (‘t-haplotypes’) to contain numerous genes, including some affecting the immune system and the development of the embryo. Rapid progress in the isolation of recombinant DNA clones for this 50 megabasepair region is generating the material for its complete molecular anatomy. A crucial step in revealing the biological functions controlled by the region is to obtain mutants in which genes are inactivated individually. We have used a pair of inbred mouse strains and a series of classical breeding schemes that permit the detection of recessive lethal and detrimental mutations in the T/t–H-2 region.

In this initial phase of our study, 280 gametes mutagenized in the male germ line by ethylnitrosourea (ENU) have yielded eleven independent pre-natal recessive lethal mutations. Four have been mapped against T mutations and have been shown to complement one another in all pairwise combinations.

Type
Research Article
Information
Genetics Research , Volume 47 , Issue 2 , April 1986 , pp. 135 - 142
Copyright
Copyright © Cambridge University Press 1986

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