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Genome organization of the Pseudomonas aeruginosa narrow host range plasmid R91–5 determined by deletion and cloning analysis

Published online by Cambridge University Press:  14 April 2009

R. J. Moore  and
V. Krishnapillai
R. J. Moore
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia
V. Krishnapillai
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia

Summary

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By physical and genetic analysis of deletion mutants of the narrow host range IncP-10 P. aeruginosa conjugative plasmid R91–5 it has been shown that the phenotypes related to its transfer, namely the inhibition of the replication of the phage G101, entry exclusion and the fertility inhibition of the wide host range plasmid R18 map at kilobase coordinates 19·7–20·7, 18·5–19·7, 28·8–30·15 and/or 34·9–36·15, respectively. These locations have been confirmed by cloning restriction enzyme generated DNA fragments of R91–5 into a small broad host range, multicopy non-conjugative plasmid.

Type
Short Paper
Information
Genetics Research , Volume 45 , Issue 2 , April 1985 , pp. 195 - 198
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

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