Hostname: page-component-77c89778f8-gvh9x Total loading time: 0 Render date: 2024-07-23T05:00:16.707Z Has data issue: false hasContentIssue false

Genetic locus determining resistance to phage BF23 and colicins E1, E2 and E3 in Escherichia coli*

Published online by Cambridge University Press:  14 April 2009

Paula Jasper
Affiliation:
Committee on Molecular Biology, Washington University St Louis, Missouri 63130
Eleanor Whitney
Affiliation:
Committee on Molecular Biology, Washington University St Louis, Missouri 63130
Simon Silver
Affiliation:
Committee on Molecular Biology, Washington University St Louis, Missouri 63130

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The gene for resistance to phage BF23 and colicins E1, E2 and E3, bfe, was mapped by a combination of conjugation and transduction crosses. Co-transduction of bfe was found with markers in the region between 76 and 79 min on the Escherichia coli genetic map. The highest frequency of co-transduction was found with argH (47%). Three-factor transductional crosses showed unambiguously that bfe lies between argH and supM, at about 77·5 min on the map.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Bhattacharyya, P., Wendt, L., Whitney, E. & Silver, S. (1970). Colicin-tolerant mutants of Escherichia coli: resistance of membranes to colicin E1. Science 168, 9981000.CrossRefGoogle ScholarPubMed
Buxton, R. S. (1971). Genetic analysis of Escherichia coli K 12 mutants resistant to bacteriophage BF 23 and the E-group colicins. Molecular and General Genetics 113, 154156.CrossRefGoogle Scholar
DeWitt, S. K. & Adelberg, E. A. (1962). The occurrence of a genetic transposition in a strain of Escherichia coli. Genetics 47, 577585.CrossRefGoogle Scholar
Eggertsson, G. (1968). Mapping of ochre suppressors in Escherichia coli. Genetical Research 11, 1520.CrossRefGoogle ScholarPubMed
Ezekiel, D. H. & Hutchins, J. E. (1968). Mutations affecting RNA polymerase associated with rifampicin resistance in Escherichia coli. Nature 220, 276277.CrossRefGoogle ScholarPubMed
Frédéricq, P. & Betz-Bareau, M. (1952). Récombinants génétiques de souches marquées par résistance aux colicines et aux bactériophages. Annales de L'Institut Pasteur 83, 283294.Google ScholarPubMed
Hell, C. & Holland, I. B. (1967). Genetic basis of colicin E susceptibility in Escherichia coli. Journal of Bacteriology 94, 677686.CrossRefGoogle Scholar
Hull, R. R. & Reeves, P. (1971). Sensitivity of intracellular bacteriophage λ to colicin CA 42-E2. Journal of Virology 8, 355362.CrossRefGoogle Scholar
Jenkin, C. R. & Rowley, D. (1955). Resistance to colicin E as a genetic marker in E. coli K 12. Nature 175, 779.CrossRefGoogle Scholar
Kaback, H. R. (1971). Bacterial membranes. In Methods in Enzymology vol. 22, (ed. Jakoby, W. B.), pp. 99120. New York: Academic Press.Google Scholar
Maeda, A. & Nomura, M. (1966). Interaction of colicins with bacterial cells. I. Studies with radioactive colicins. Journal of Bacteriology 91, 685694.CrossRefGoogle ScholarPubMed
Morrissey, A. T. E. & Fraenkel, D. G. (1969). Chromosomal location of a gene for fructose 6-phosphate kinase in Escherichia coli. Journal of Bacteriology 100, 11081109.CrossRefGoogle ScholarPubMed
Nagel de Zwaig, R. & Luria, S. E. (1967). Genetics and physiology of colicin-tolerant mutants of Escherichia coli. Journal of Bacteriology 94, 11121123.CrossRefGoogle ScholarPubMed
Nomura, M. (1964). Mechanism of action of colicines. Proceedings of the National Academy of Sciences, U.S.A. 52, 15141521.CrossRefGoogle ScholarPubMed
Nomura, M. (1967). Colicins and related bacteriocins. Annual Review of Microbiology 21, 257284.CrossRefGoogle ScholarPubMed
Nomura, M. & Witten, C. (1967). Interaction of colicins with bacterial cells. III. Colieintolerant mutations in Escherichia coli. Journal of Bacteriology 94, 10931111.CrossRefGoogle ScholarPubMed
Orias, E., Gartner, T. K., Lannan, J. E. & Betlach, M. (1972). Close linkage between ochre and missense suppressors in Escherichia coli. Journal of Bacteriology 109, 11251133.CrossRefGoogle ScholarPubMed
Pfaff, P. L. & Whitney, E. N. (1971). Map position of the mutation for colicin E resistance in Escherichia coli: the cer gene. Bacteriological Proceedings 1971, p. 50 (Abstract).Google Scholar
Reeves, P. (1966). Mutants resistant to colicin CA42-E2: cross resistance and genetic mapping of a special class of mutants. Australian Journal of Experimental Biology and Medical Science 44, 301316.CrossRefGoogle ScholarPubMed
Taylor, A. L. (1970). Current linkage map of Escherichia coli. Bacteriological Reviews 34, 155175.CrossRefGoogle ScholarPubMed
Whitney, E. N. (1970). Genetics of the tolC locus of Escherichia coli K 12. Ph.D. Thesis, Biology Department, Washington University, St Louis, Mo.Google Scholar
Whitney, E. N. (1971). The tolC locus in Escherichia coli K12. Genetics 67, 3953.CrossRefGoogle ScholarPubMed