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Genetic background affects expression of t haplotype in mouse sperm

Published online by Cambridge University Press:  14 April 2009

Patricia Olds-Clarke  and
Susan McCabe
Patricia Olds-Clarke
Affiliation:
Department of Anatomy, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
Susan McCabe
Affiliation:
Department of Anatomy, Temple University School of Medicine, Philadelphia, PA 19140, U.S.A.
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Summary

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Two aspects of sperm phenotype were examined for tw32/+ males of the inbred strains C3H and C57BL/6 (B6). Sperm from fertile C3H-tw32/+ males very rarely achieved fertilization in vitro, while sperm from congenie C3H-+/+ males had no such difficulties. The presence of tw32 had no effect on ability of B6 sperm to undergo fertilization in vitro. In fertile hybrid males produced from crosses of B6 and C3H strains, tw32 significantly reduced, but did not inhibit completely, the sperms' ability to fertilize in vitro. The presence of tw32 decreased by a factor of two the frequency of abnormal sperm heads in males of the B6 strain, but doubled the frequency in the C3H strain. Hybrid males resembled the C3H strain in this respect. The presence of T or T2J had no effect on the level of sperm abnormalities in any strain. These results emphasize the importance of genetic background in expression of t haplotypes in sperm, and suggest that tw32 can influence a range of sperm characteristics, by interacting with products of other loci.

Type
Research Article
Information
Genetics Research , Volume 40 , Issue 3 , December 1982 , pp. 249 - 254
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Beatty, A. (1970). The genetics of the mammalian gamete. Biological Reviews 45, 71–119.Google Scholar
Bennett, D. (1964). Selection toward normality in genetically tailless mice, and some correlated effects. American Naturalist 98, 5–11.Google Scholar
Bennett, D. (1975). The T locus of the mouse. Cell 6, 441–454.Google Scholar
Braden, A. (1972). T-locus in mice: segregation distortion and sterility in the male. In The Genetics of the Spermatozoan. Proc. Int. Symp. (ed. Beatty, R. A. and Gluecksohn-Waelsch, S.), pp. 289–305. Edinburgh and New York.Google Scholar
Bryson, V. (1944). Spermatogenesis and fertility in Mus musculus as affected by factors at the T locus. Journal of Morphology 74, 131–185.Google Scholar
Carey, J. & Olds-Clarke, P. (1980). Differences in sperm function in vitro but not in vivo between inbred and random-bred mice. Gamete Research 3, 9–15.Google Scholar
Dooher, G. & Bennett, D. (1977). Spermiogenesis and spermatozoa in sterile mice carrying different lethal T/t locus haplotypes: a transmission and scanning electron microscopic study. Biology of Reproduction 17, 269–288.Google Scholar
Eicher, E., Cherry, M. & Flaherty, L. (1978). Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex. Molecular and General Genetics 158, 225–228.Google Scholar
Ginsberg, L. & Hillman, N. (1974). Meiotic drive in tn-bearing mouse spermatozoa: a relationship between aerobic respiration and transmission frequency. Journal of Reproduction and Fertility 38, 157–163.CrossRefGoogle ScholarPubMed
Hammerberg, C. & Klein, J. (1975). Linkage disequilibrium between H-2 and t complexes in chromosome 17 of the mouse. Nature 258, 296–299.CrossRefGoogle Scholar
Hillman, N. & Nadijcka, M. (1978). A study of spermatozoan defects in wild-type and T:t-bearing mice. Journal of Embryology and Experimental Morphology 44, 263–280.Google Scholar
Katz, D., Erickson, R. & Nathanson, M. (1979). Beat frequency is bimodally distributed in spermatozoa from T/t 12 mice. Journal of Experimental Zoology 210, 529–535.Google Scholar
Klein, J. & Hammerberg, C. (1977). The control of differentiation by the T complex. Immunological Reviews 33, 70–104.Google Scholar
Krzanowska, H. (1974). The passage of abnormal spermatozoa through the uterotubal junction of the mouse. Journal of Reproduction and Fertility 38, 81–90.Google Scholar
Lyon, M., Evans, E., Jarvis, S. & Sayers, I. (1979). t-Haplotypes of the mouse may involve a change in intercalary DNA: Nature 279, 38–42.CrossRefGoogle ScholarPubMed
Micková, M. & Iványi, P. (1974). Sex-dependent and H-2 linked influence on expressivity of the brachyury gene in mice. Journal of Heredity 65, 369–372.Google Scholar
Nadijcka, M. & Hillman, N. (1980). Differences in cAMP levels in tn- and non-tn-bearing mouse spermatozoa. Biology of Reproduction 22, 1102–1105.Google Scholar
Olds-Clarke, P. & Becker, A. (1978). The effect of the T/t locus on sperm penetration in vivo in the house mouse. Biology of Reproduction 18, 132–140.Google Scholar
Olds-Clarke, P. & Carey, J. (1978). Rate of egg penetration in vitro accelerated by T/t locus in the mouse. Journal of Experimental Zoology 206, 323–331.Google Scholar
Sherman, M. & Wudl, L. (1977). T-complex mutations and their effects. In: Concepts in Mammalian Embryogenesis. Cell Monograph Series No. 1. (ed. Sherman, M. I.), pp. 136–234., The MIT Press.Google Scholar
Silver, L., Artzt, K. & Bennett, D. (1979). A major testicular cell protein specified by a mouse T/t complex gene. Cell 17, 275–284.Google Scholar
Silver, L., White, M. & Artzt, K. (1980). Evidence for unequal crossing over within the mouse T/t complex. Proceedings of the National Academy of Science, U.S.A. 77, 6077–6080.Google Scholar
Smith, D., Oura, C. & Zamboni, L. (1970). Fertilizing ability of structurally abnormal spermatozoa. Nature 227, 79–80.Google Scholar
Vandebero, J. & Klein, J. (1978). Localization of mouse Pgk-2 gene at the D end of the H-2 complex. Journal of Experimental Zoology 203, 319–324.Google Scholar
Yanagisawa, K. (1965). Studies on the mechanism of abnormal transmission ratios at the T-locus in the house mouse. II. Test for physiological differences between t- and T-bearing sperm manifested in vitro. Japanese Journal of Genetics 40, 87–92.Google Scholar