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Galactosyltransferase activities on mouse sperm bearing multiple tlethal and tviable haplotypes of the T/t-complex

Published online by Cambridge University Press:  14 April 2009

Barry D. Shur
Barry D. Shur
Affiliation:
Department of Anatomy, University of Connecticut Health Center, Farmington, Connecticut 06032, U.S.A.

Summary

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Segregation-distorting t-sperm show a specific increase in N-acetylglucosamine: galactosyltransferase activity over wild-type (+/+) due to a deficiency of a wild-type galactosyltransferase inhibitor (Shur & Bennett, 1979). Eight other enzymic activities are indistinguishable between +- and t-sperm suspensions. In this study, three additional points are analysed. First, galactosyltransferases are assayed on sperm homozygous for a semilethal haplotype (tW2/tW2), relative to heterozygous (+ /tw2) and wild-type (+/+) controls. tW2/tW2 assays circumvent the +-sperm inhibition of t-sperm galactosyltransferases that occurs in heterozygous + /t-assays and show that t-sperm are actually four times as active as wild-type. Second, sperm which are compound heterozygotes for two complementing lethal t-haplotypes (tlx/tly), have nearly twice the theoretical enzyme level of tlx/tly sperm. Thus, in either homozygous (tW2/tW2) or double heterozygous (tlx/tly) form t-haplotypes act synergistically on sperm galactosyltransferase activity.

Third, and most interesting, sperm bearing either recombinant, viable t-haplotypes (+/tv, tvx/tvy), or one of three dominant T/t-complex mutations, were assayed to determine which portions of the T/t-complex are responsible for elevated galactosyltransferase activity. Results show that sperm bearing recombinant, non-segregation-distorting, viable tv-haplotypes no longer show elevated transferase activity. Therefore, the elevated t1-sperm galactosyltransferase activity strictly correlates with the increased transmission frequency of t1-sperm. These studies strengthen further the hypothesis that sperm surface galactosyltransferases are involved in egg binding during fertilization, and that t1-sperm segregation-distortion results, at least in part, from increased galactosyltransferase activity.

Type
Research Article
Information
Genetics Research , Volume 38 , Issue 3 , December 1981 , pp. 225 - 236
Copyright
Copyright © Cambridge University Press 1981

References

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