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Definition of the blue mutant phenotype and its genetic basis in Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

I. de G. Mitchell  and
E. A. Bevan
I. de G. Mitchell
Affiliation:
Department of Plant Biology and Microbiology, Queen Mary College, London University, London, E.1
E. A. Bevan
Affiliation:
Department of Plant Biology and Microbiology, Queen Mary College, London University, London, E.1
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Mutant colonies of yeast are described which varied in colour from blue to green and in intensity of colour when grown on medium containing methylene blue. Blue mutant colonies contained more dead cells, decolorized redox dyes more slowly, had a higher respiratory quotient on glucose containing medium, and absorbed more methylene blue dye than did wild-type. The blue colony phenotype was induced both spontaneously and at high frequency by ultraviolet light and ethylmethane-sulphonate in both haploid and diploid strains. Many of the light-coloured colonies isolated following mutagenic treatment reverted to wild-type when subcultured but most dark-coloured colonies remained stable. Stable blue mutants were shown to arise by mutation of many separate nuclear genes in haploids and were often caused by recessive lethals in diploids.

Type
Research Article
Information
Genetics Research , Volume 22 , Issue 2 , October 1973 , pp. 169 - 179
Copyright
Copyright © Cambridge University Press 1973

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