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Cytogenetic analysis of the echinoid (ed), dumpy (dp) and clot (cl) region in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

János Szidonya  and
Gunter Reuter
János Szidonya*
Affiliation:
Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6701, Szeged POB 521, Hungary.
Gunter Reuter
Affiliation:
Department of Genetics, Martin Luther University, DDR-402 Halle/S. Domplatz 1, German Democratic Republic
*
* Corresponding author.
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Summary

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The chromosomal region surrounding the ed, dp and cl genes has been studied cytogenetically (24–26 on 2L chromosome). It contains three Minutes and a haplo-sterile function. For isolation of deficiencies and mutations these haplo-insufficient functions were covered by an insertional translocation of 24D4-25F2 into the X chromosome, or by tandem duplications. 112 lethal and visible mutations induced by EMS and X-rays have been localized by deficiency mapping to 20 subregions. They specify 42 loci in a 48 band interval consistent with the notion that most of the bands encode a single lethal function. The dp, DTS, tkv and suppressor/enhancer loci for position-effect variegation were studied in detail. A dominant suppressor function was localized within the structural part of the dp complex. New non-conditional lethals have been isolated for the DTS locus. Complementation analysis with the previously identified dominant heat-sensitive alleles places the site for heat sensitivity in the middle of the locus. Two haplo-abnormal enhancers of position-effect variegation were localized in the region 25F2–26A1. A triplo-abnormal suppressor function maps to 26B2–5; 26B9. The dose-dependent functions of these loci were studied by the use of deficiencies and duplications.

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 3 , June 1988 , pp. 197 - 208
Copyright
Copyright © Cambridge University Press 1988

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