Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-26T04:00:38.646Z Has data issue: false hasContentIssue false
  • English
  • Français

Covalently closed, circular DNA in kappa endosymbionts of Paramecium

Published online by Cambridge University Press:  14 April 2009

Judith A. Dilts
Judith A. Dilts
Affiliation:
Department of Zoology, Indiana University, Bloomington, Indiana 47401, U.S.A.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Caedobacter taeniospiralis (kappa), a bacterial endosymbiont isolated from Paramecium tetraurelia stock 51, contains, in addition to the bacterial chromosome, covalently closed circular DNA molecules as shown by isolation on dye-buoyant-density gradients. The closed circular molecule has a contour length of 13·75 ± 0·04 µm with a buoyant density of 1·698 g/cm3. The buoyant density of the bacterial chromosome is 1·700–1·701 g/cm3. Kappa of the 51 group isolated from stock 298 and stock 6g2, P. tetraurelia, also contain the closed circular DNA. Two forms of kappa coexist in paramecia: brights and nonbrights. Examination by density-gradient centrifugation of the DNA of brights and nonbrights shows the extrachromosomal DNA to be associated mainly with brights. It is suggested that the extrachromosomal DNA might be the determinant for the refractile bodies and the helical phage-like structures found in brights.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 161 - 170
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Bazaral, M. & Helinski, D. R. (1968). Circular DNA forms of colicinogenic factors El, E2 and E3 from Escherichia coli. Journal of Molecular Biology 36, 185–194.Google Scholar
Dilts, J. A. (1974). Evidence for covalently closed, circular DNA in the kappa endosymbiont of stock 51 of Paramecium aurelia. Genetics 77 (Suppl.), s 17–18.Google Scholar
Gordon, C. N. & Kleinschmidt, A. K. (1968). High contrast staining of individual nucleic acid molecules. Biochimica et Biophysica Acta 155, 305–307.Google Scholar
Grimes, G. W. & Preer, J. R. Jr (1971). Further observations on the correlation between kappa and phage-like particles in Paramecium. Genetical Research 18, 115–116.CrossRefGoogle Scholar
Helinski, D. R. & Clewell, D. B. (1971). Circular DNA. Annual Review of Biochemistry 40, 899–937.Google Scholar
Lang, D. (1970). Molecular weights of coliphages and coliphage DNA. III. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from Bovine Papilloma Virus. Journal of Molecular Biology 54, 557–565.CrossRefGoogle Scholar
Lang, D. & Mitani, M. (1970). Simplified quantitative electron microscopy of biopolymers. Biopolymers 9, 373–379.CrossRefGoogle ScholarPubMed
Preer, J. R. Jr & Jurand, A. (1968). The relation between virus-like particles and R bodies of Paramecium aurelia. Genetical Research 12, 331–340.CrossRefGoogle Scholar
Preer, J. R. Jr & Preer, L. B. (1967). Virus-like bodies in killer paramecia. Proceedings of the National Academy of Sciences of the United States of America 58, 1774–1781.Google Scholar
Preer, J. R. Jr, Preer, L. B. & Jurand, A. (1974). Kappa and other endosymbionts in Paramecium aurelia. Bacteriological Reviews 38, 113–163.CrossRefGoogle ScholarPubMed
Preer, J. R. Jr, Preer, L. B., Rudman, B. & Jurand, A. (1971). Isolation and composition of bacteriophage-like particles from kappa of killer paramecia. Molecular and General Genetics 110, 202–208.Google Scholar
Preer, L. B., Rudman, B. M., Preer, J. R. Jr & Jurand, A. (1974). Induction of R bodies by ultraviolet light in killer paramecia. Journal of General Microbiology 80, 209–215.Google Scholar
Radloff, R., Bauer, W. & Vinograd, J. (1967). A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57, 1541–1521.Google ScholarPubMed
Singler, M. J. (1974). Antigenic studies on the relationship of the viral capsid, R body and toxin of kappa in stock 562, Paramecium aurelia. Genetics 77 (Suppl.), s 60–61.Google Scholar
Singler-Bastiaans, M. J. (1975). Studies with killer paramecia. I. The relationship of the R body and toxin to the phage in killer paramecia. Ph.D. Thesis, Indiana University, U.S.A.Google Scholar
Smith-Sonneborn, J. E., Green, L. & Marmur, J. (1963). Deoxyribonucleic acid base composition of kappa and Paramecium aurelia, stock 51. Nature 197, 385.Google Scholar
Sonneborn, T. M. (1938). Mating types, toxic interactions and heredity in Paramecium aurelia. Science 88, 503.Google Scholar
Sonneborn, T. M. (1970). Methods in Paramecium research. Methods in Cell Physiology 4, 241–339.CrossRefGoogle Scholar
Suyama, Y. & Miura, K. (1968). Size and structural variations of mitochondrial DNA. Proceedings of the National Academy of Sciences of the United States of America 60, 235–242.CrossRefGoogle ScholarPubMed
Suyama, Y. & Preer, J. R. Jr (1965). Mitochondrial DNA from protozoa. Genetics 52, 1015–1058.Google Scholar
Watson, R., Bauer, W. & Vinograd, J. (1971). An optical system for the photography of fluorescent bands in preparative ultracentrifuge tubes. Analytical Biochemistry 44, 200–206.Google Scholar