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Conditional induction of λ prophage in exrA mutants of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

J. J. Donch ,
M. H. L. Green  and
J. Greenberg
J. J. Donch
Affiliation:
Palo Alto Medical Research Foundation, Pala Alto, California 94301
M. H. L. Green
Affiliation:
Palo Alto Medical Research Foundation, Pala Alto, California 94301
J. Greenberg
Affiliation:
Palo Alto Medical Research Foundation, Pala Alto, California 94301

Summary

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Strains of Escherichia coli which contain the UV sensitivity gene exrA, repress UV induction of λ prophage in a novel manner. While exrA+ strains can be induced by UV irradiation and express this induction in a 2 h period, exrA mutants delay the expression of induction for almost 4 h, and the induction maximum is approximately 10% of the wild-type value. The kinetics of the delayed induction and superinfection experiments indicate that the lifting of immunity and induction of λ prophage occur simultaneously.

Type
Short Papers
Information
Genetics Research , Volume 17 , Issue 2 , April 1971 , pp. 161 - 163
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Brooks, K. & Clark, A. J. (1967). Behaviour of λ bacteriophage in a recombination deficient strain of Escherichia coli. Journal of Virology 1, 283–293.CrossRefGoogle Scholar
Donch, J. & Greenberg, J. (1968). Loci of radiation sensitivity in Bs strains of Escherichia coli. Genetical Research, Cambridge 11, 183–191.CrossRefGoogle Scholar
Donch, J., Green, M. H. L. & Greenberg, J. (1968). Interaction of the exr and lon genes in Escherichic coli. Journal of Bacteriology 96, 1704–1710.Google Scholar
Donch, J., Greenberg, J. & Green, M. H. L. (1970). Repression of induction by U.V. of λ phage by exrA mutations in Escherichia coli. Genetical Research, Cambridge 15, 87–97.Google Scholar
Green, M. H. L., Greenberg, J. & Donch, J. (1969). Effect of a recA gene on cell division and capsular polysaccharide production in a lon strain of Escherichia coli. Genetical Research, Cambridge 14, 159–162.Google Scholar
Howard-Flanders, P., Rupp, W. D., Wilkins, B. M. & Cole, R. S. (1968). DNA replication and recombination after UV-irradiation. Cold Spring Harbor Symposia of Quantitative Biology 33, 195–207.CrossRefGoogle ScholarPubMed
Iwo, K. (1968). Photoreactivation spectra for prophage-inducing UV damage and delayed appearance of induced prophage in Escherichia coli. Japan Journal of Genetics 43, 257–270.Google Scholar
Matsubara, K. & Kaiser, A. D. (1968). λdv: an autonomously replicating DNA fragment. Cold Spring Harbor Symposia of Quantitative Biology 33, 769–775.Google Scholar
Mattern, I. E., Zwenk, H. & Rörsch, A. (1966). The genetic constitution of the radiation sensitive mutant E. coli Bs-1. Mutation Research 3, 374–380.CrossRefGoogle Scholar
Rörsch, A., Van De Putte, P., Mattern, I. E., Zwenk, H. & Van Sluis, C. A. (1966). In Genetical Aspects of Radiosensitivity: Mechanisms of Repair (Proc. Panel Vienna, 04, 1966). IAEA, pp. 105–129.Google Scholar
Sussman, R. & Jacob, F. (1962). Sur un système de repression thermosensible chez le bacteriophage λ d'Escherichia coli. Compte rendue hebdomaire des Séances de l'Académie de Science, Paris 254, 1517–1519.Google Scholar
Witkin, E. M. (1967). Mutation-proof and mutation-prone modes of survival in derivatives of Escherichia coli B differing in sensitivity to ultraviolet light. Brookhaven Symposia on Biology 20, 17–55.Google Scholar
Witkin, E. M. (1969). The mutability toward ultraviolet light of recombination-deficient strains of Escherichia coli. Mutation Research 8, 9–14.CrossRefGoogle ScholarPubMed