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Combined chromosomal and plasmid encoded control for the degradation of phenol in Pseudomonas putida

Published online by Cambridge University Press:  14 April 2009

C. L. Wong  and
N. W. Dunn
C. L. Wong
Affiliation:
School of Biological Technology, University of New South Wales, P.O. Box 1, Kensington, New South Wales, 2033, Australia
N. W. Dunn
Affiliation:
School of Biological Technology, University of New South Wales, P.O. Box 1, Kensington, New South Wales, 2033, Australia
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Summary

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The TOL(M1) metabolic plasmid was transferred from Pseudomonas arvilla mt-2 to a mutant of Pseudomonas putida. Although neither the donor nor the recipient could grow on phenol, the transconjugants could grow slowly on this carbon source. In these transconjugants phenol was converted to catechol by chromosomal encoded phenol hydroxylase followed by degradation of catechol by low uninduced levels of the plasmid encoded catechol meta cleavage pathway. A mutant, which grew well on phenol, was isolated from one of the transconjugants and it was found that phenol could now act as an inducer for the meta cleavage pathway.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 3 , June 1976 , pp. 405 - 412
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

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