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Cell autonomy of two DNA-repair mutations in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Pedro Ripoll  and
James A. Kennison
Pedro Ripoll
Affiliation:
Centro de Biologia Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain
James A. Kennison
Affiliation:
Centro de Biologia Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain

Summary

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By means of genetic mosaics we have studied the cell autonomy of mei-41 and mei-9, two loci involved in DNA metabolism. The frequency of spontaneous somatic spots resulting from unrepaired chromosome damage and the sensitivity of mutant cells to killing by X-irradiation - two traits indicative of deficient DNA repair - have been analysed at the cellular level. The results show that: (1) The effect of both mutations on chromosome stability is cell autonomous. (2) After 1000 r of X-irradiation practically all the cells homozygous for mei-41 disappear while about one-third of the cells homozygous for mei-9a survive the irradiation. The possibility of using these mutants as tools to approach developmental problems is discussed.

Type
Research Article
Information
Genetics Research , Volume 46 , Issue 1 , August 1985 , pp. 31 - 44
Copyright
Copyright © Cambridge University Press 1985

References

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