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Application of multiplex immunoassay technology to investigations of ocular disease

  • Valerie Sloane Jones (a1), Jian Wu (a2), Si-Wei Zhu (a3) and Ruo-Pan Huang (a1) (a3) (a4)


Eye-derived fluids, including tears, aqueous humour and vitreous humour often contain molecular signatures of ocular disease states. These signatures can be composed of cytokines, chemokines, growth factors, proteases and soluble receptors. However, the small quantities (<10 µl) of these fluids severely limit the detection of these proteins by traditional enzyme-linked immunosorbent assay or Western blot. To maximise the amount of information generated from the analysis of these specimens, many researchers have employed multiplex immunoassay technologies for profiling the expression or modification of multiple proteins from minute sample volumes.


Corresponding author

*Corresponding author: Ruo-Pan Huang, Raybiotech, Inc, 3607 Parkway Lane, Suite 100, Norcross, GA 30092, USA. E-mail:


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