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Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases

Published online by Cambridge University Press:  25 January 2001

Donna Otter  and
Clare Austin
Donna Otter
Affiliation:
Department of Medicine, Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, UK
Clare Austin
Affiliation:
Department of Medicine, Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, UK
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Abstract

We report the first simultaneous measurements of pHi, [Ca2+]i and tension, upon alteration of pHi, in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCl) alterations of pHi, by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pHi they were, at all times, accompanied by parallel changes in [Ca2+]i. Experimental Physiology (2000) 85.3, 349-351.

Type
Research Article
Information
Experimental Physiology , Volume 85 , Issue 3 , May 2000 , pp. 349 - 351
Copyright
© The Physiological Society 2000

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