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Pancreatic exocrine secretions as a source of luminal polyamines in pigs

Published online by Cambridge University Press:  25 January 2001

Suzanne Loret ,
Philippe Brolet ,
Stefan Pierzynowski ,
Isabelle Gouders ,
Monique Klimek ,
Viggo Danielson ,
Anne Rosted ,
Violetta Lesniewska  and
Guy Dandrifosse
Suzanne Loret
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Philippe Brolet
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Stefan Pierzynowski
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Isabelle Gouders
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Monique Klimek
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Viggo Danielson
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Anne Rosted
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Violetta Lesniewska
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
Guy Dandrifosse
Affiliation:
Department of Biochemistry and General Physiology, University of Liege, Sart Tilman B6c, B-4000 Liege, Belgium Danish Institute of Agricultural Science, Department of Animal Nutrition and Physiology, PO Box 50, DK 8830 Tjele, Denmark Gramineer Institute AB, Ideon, S 22370 Lund and Department of Animal Physiology, Lund University, Helgonavägen 3b, S 22362 Lund, Sweden
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Abstract

The goal of the present study was twofold: (1) to detect the possible storage of dietary polyamines (PAs) in various tissues and (2) to investigate the role of dietary PAs in the differentiation of the pig intestinal epithelium. A first experimental series was designed to assess the accumulation of either milk PAs (mostly spermidine) or orally administered spermine (SPM) in piglet red blood cells (RBCs) and plasma, a preliminary stage in their distribution to growing and storage organs. Though PA concentrations of piglet RBCs and plasma were generally significantly higher than their sow counterparts, our experimental conditions failed to demonstrate that this increase could stem from ingested PAs. A second experimental series dealt with the determination of disaccharidase specific activities in proximal and distal parts of piglet gut on the 26th and 29th days after birth (preweaning time). In agreement with observations made previously on rat pups, we observed an increase in maltase specific activity (SA) at the end of the suckling period (the observed increase in sucrase SA was not significant). However, orally administered SPM did not affect this activity. Compared to the constant protein concentrations observed in both parts of the gut, the pancreatic protein content decreased sharply between the 26th and 29th postnatal days. At the same time pancreatic concentrations of spermidine (SPD) also decreased, suggesting that some pancreatic PAs were released as the organ secreted its proteins. In accordance with this hypothesis, we recorded SPM and SPD in pancreatic juice. The increases in PA concentrations seemed to follow the protein secretion pattern (i.e. PA concentrations reached a maximal value when the protein concentration was highest). The presence of PAs in pancreatic juice could be indicative of a control mechanism exerted by the pancreas on PA-induced growth and differentiation of porcine intestinal epithelium. Experimental Physiology (2000) 85.3, 301-308.

Type
Research Article
Information
Experimental Physiology , Volume 85 , Issue 3 , May 2000 , pp. 301 - 308
Copyright
© The Physiological Society 2000

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