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RELATIONSHIP BETWEEN ENDOGENOUS SALICYLIC ACID AND ANTIOXIDANT ENZYME ACTIVITIES IN MAIZE SEEDLINGS UNDER CHILLING STRESS

Published online by Cambridge University Press:  09 January 2013

YANG WANG ,
TINGTING WEN ,
JIN HU ,
RUI HAN ,
YANFANG ZHU ,
YAJING GUAN  and
SHUIJIN ZHU
YANG WANG
Affiliation:
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
TINGTING WEN
Affiliation:
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
JIN HU*
Affiliation:
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
RUI HAN
Affiliation:
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
YANFANG ZHU
Affiliation:
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
YAJING GUAN
Affiliation:
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
SHUIJIN ZHU
Affiliation:
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
*
†Corresponding author. Email: jhu@zju.edu.cn
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Summary

Salicylic acid (SA) can induce multiple stress tolerance in plants. This study investigated the relationship between SA and antioxidant enzyme activities in maize seedlings under chilling stress. Changes of endogenous SA, antioxidant enzyme activities and malondialdehyde (MDA) concentrations were assessed in two different chilling-tolerant maize inbred lines (Huang C and Mo17) under chilling stress. The results showed that both endogenous free and bound salicylic acid contents increased in roots and leaves of both lines. MDA concentrations also increased significantly in roots and leaves of both lines after chilling stress. In addition, in Huang C, chilling stress increased the activities of four antioxidant enzymes, ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and peroxidase, while in Mo17, only CAT and APX increased. Furthermore, a regression analysis was conducted between SA and MDA concentrations or antioxidant enzyme activities under chilling stress. The results indicated that MDA concentrations were positively correlated with total SA contents in roots (r = 0.9776, p = 0.0224) and bound SA in leaves (r = 0.9974, p = 0.0458), respectively. Total SA contents had positive correlations with APX activities both in roots (r = 0.9993, p = 0.002) and leaves (r = 0.9630, p = 0.037) and GR in leaves (r = 0.9298, p = 0.0221). Together, these results suggested that chilling stress improved the biosynthesis of endogenous SA, and lipid peroxidation and antioxidant enzyme activities could be indicated by endogenous SA contents of maize seedlings under chilling stress. Furthermore, increased activities of antioxidant enzymes, especially in roots, may contribute to the chilling tolerance of maize seedlings.

Type
Research Article
Information
Experimental Agriculture , Volume 49 , Issue 2 , April 2013 , pp. 295 - 308
Copyright
Copyright © Cambridge University Press 2013

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References

REFERENCES

Alscher, R. G., Donahue, J. and Cramer, L. L. (1997). Reactive oxygen species and antioxidants: Relationships in green cells. Physiologia Plantarum 100:224–233.CrossRefGoogle Scholar
Cao, D. D., Hu, J., Zhu, S. J., Hu, W. M. and Knapp, A. (2010). Relationship between changes in endogenous polyamines and seed quality during development of sh2 sweet corn (Zea mays L.) seed. Scientia Horticulturae 123:301–307.CrossRefGoogle Scholar
Dat, J. F., Foyer, C. H. and Scott, I. M. (1998). Changes in salicylic acid and antioxidants during induced thermotolerance in mustard seedlings. Plant Physiology 118:1455–1461.Google Scholar
Drazic, G. and Mihailovic, N. (2005). Modification of cadmium toxicity in soybean seedlings by salicylic acid. Plant Science 168:511–517.Google Scholar
Gao, C. H., Hu, J., Zhang, S., Zheng, Y. Y. and Knapp, A. (2009). Association of polyamines in governing the chilling sensitivity of maize genotypes. Plant Growth Regulation 57:31–38.CrossRefGoogle Scholar
Guan, Y. J., Hu, J., Wang, X. J. and Shao, C. X. (2009). Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University Science B 10:427–433.Google Scholar
Gunes, A., Inal, A., Alpaslan, M., Eraslan, F., Bagci, E. G. and Cicek, N. (2007). Salicylic acid-induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. Journal of Plant Physiology 164:728–736.Google Scholar
Hodges, D. M., Andrews, C. J., Johnson, D. A. and Hamilton, R. I. (1997). Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines. Journal of Experimental Botany 48:1105–1113.Google Scholar
Holá, D., Kočová, M., Rothová, O., Wilhelmová, N. and Benešová, M. (2007). Recovery of maize (Zea mays L.) inbreds and hybrids from chilling stress of various duration: Photosynthesis and antioxidant enzymes. Journal of Plant Physiology 164:868–877.CrossRefGoogle ScholarPubMed
Horváth, E., Janda, T., Szalai, G. and Páldi, E. (2002). In vitro salicylic acid inhibition of catalase activity in maize: Differences between the isozymes and a possible role in the induction of chilling tolerance. Plant Science 163:1129–1135.CrossRefGoogle Scholar
Horváth, E., Szalai, G. and Janda, T. (2007). Induction of abiotic stress tolerance by salicylic acid signaling. Journal of Plant Growth Regulation 26:290–300.CrossRefGoogle Scholar
Hu, J., Xie, X. J., Wang, Z. F. and Song, W. J. (2006). Sand priming improves alfalfa germination under high-salt concentration stress. Seed Science Technology 34:199–204.Google Scholar
Kuk, Y. I., Sin, J. S., Burgos, N. R., Hwang, T. E., Han, O., Cho, B. H., Jung, S. and Guh, J. O. (2003). Antioxidative enzymes offer protection from chilling damage in rice plants. Crop Science 43:2109–2117.Google Scholar
Lee, D. L. and Lee, C. B. (2000). Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: In gel enzyme activity assays. Plant Science 159:75–85.Google Scholar
Liu, J. J., Lin, S. H., Xu, P. L., Wang, X. J. and Bai, J. G. (2009). Effects of exogenous silicon on the activities of antioxidant enzymes and lipid peroxidation in chilling-stressed cucumber leaves. Agricultural Sciences in China 8:1075–1086.Google Scholar
Morsy, M. R, Jouve, L., Hausman, J.-F., Hoffmann, L. and Stewart, J. M. (2007). Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. Journal of Plant Physiology 164:157–167.Google Scholar
Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 22:867–880.Google Scholar
Ohashi, Y., Murakami, T., Mitsuhara, I. and Seo, S. (2004). Rapid down and upward translocation of salicylic acid in tobacco plants. Plant Biotechnology 21:95–101.Google Scholar
Pál, M., Horváth, E., Janda, T., Páldi, E. and Szalai, G. (2005). Cadmium stimulates the accumulation of salicylic acid and its putative precursors in maize (Zea mays) plants. Physiologia Plantarum 125:356–364.Google Scholar
Pinhero, R. G., Rao, M. V., Paliyath, G., Murr, D. P. and Fletcher, R. A. (1997). Changes in activities of antioxidant enzymes and their relationship to genetic and paclobutrazol-induced chilling tolerance of maize seedlings. Plant Physiology 114:695–704.Google Scholar
Posmyk, M. M. and Janas, K. M. (2007). Effects of seed hydropriming in presence of exogenous proline on chilling injury limitation in Vigna radiata L. seedlings. Acta Physiologia Plantarum 29:509–517.CrossRefGoogle Scholar
Sawada, H., Shim, I. S. and Usui, K. (2006). Induction of benzoic acid 2-hydroxylase and salicylic acid biosynthesis-modulation by salt stress in rice seedlings. Plant Science 171:263–270.CrossRefGoogle Scholar
Shi, Q., Bao, Z., Zhu, Z., Ying, Q. and Qian, Q. (2006). Effects of different treatments of salicylic acid on heat tolerance, chlorophyll fluorescence, and antioxidant enzyme activity in seedlings of Cucumis sativa L. Plant Growth Regulation 48:127–135.Google Scholar
Smith, I. K., Vierheller, T. L. and Thorne, C. A. (1988). Assay of glutathione reductase in crude tissue homogenates using 5, 5’-dithiobis (2-nitrobenzoic acid). Analytical Biochemistry 175:408–413.Google Scholar
Szalai, S., Horgosi, S., Soos, V., Majlath, I., Balazs, E. and Janda, T. (2011). Salicylic acid treatment of pea seeds induces its de novo synthesis. Journal of Plant Physiology 168:213–219.Google Scholar
Turan, Ö. and Ekmekçi, Y. (2011). Activities of photosystem II and antioxidant enzymes in chickpea (Cicer arietinum L.) cultivars exposed to chilling temperatures. Acta Physiologiae Plantarum 33:67–78.Google Scholar
Vicente, M. R. and Plasencia, J. (2011). Salicylic acid beyond defence: Its role in plant growth and development. Journal of Experimental Botany 62:3321–3338.Google Scholar
Wang, Y. Y., Mopper, S. and Hasenstein, K. H. (2001). Effects of salinity on endogenous ABA, IAA, JA, and SA in Iris hexagona. Journal of Chemistry Ecology 27:327–342.Google Scholar
Xu, P. L, Guo, Y. K, Bai, J. G, Shang, L. and Wang, X. J. (2008). Effects of long-term chilling on ultrastructure and antioxidant activity in leaves of two cucumber cultivars under low light. Physiologia Plantarum 132:467–478.Google Scholar
Zawoznik, M. S., Groppa, M. D., Tomaro, M. L. and Benavides, M. P. (2007). Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana. Plant Science 173:190–197.Google Scholar
Zheng, Y. Y., Hu, J, Zhang, S. and Gao, C. H. (2006). The identification of chilling-tolerance in maize inbred lines at germination and seedling growth stages. Journal of Zhejiang University (Agriculture Life Science) 32:41–45 (in Chinese with an English abstract).Google Scholar
Zhu, G. and Zhong, W. (1990). Plant Physiological Experiment. Beijing: Peking University Press.Google Scholar